This study was aimed to  detect and analysis of mutL gene from Pseudomonas aeruginosa in UTI patients .Two hundred midstream urine specimens were collected from UTI patients. Laboratory diagnosis carried out depend on morphological and biochemical tests and confirmed by using VITEK- 2 Compact system. The results showed twenty isolates of  Pseudomonas aeruginosa from two hundred urine specimens in percentage (10%). Genome extracted from all twenty isolates by specific kit , then amplification of mutL gene in PCR by specific primers. Sequencing process

The Maintaining the temperature system for fuel diesel engine by mathematical modeling. Thermal control of fuel with objective of this research, Peltier thermal application and paraffin-based thermal batteries. A fuel system thermometer is assembled in the diesel engine and thermal controller. A thermal model for thermal control of fuel has been developed in the diesel engine. Mathematical model is a system of differential equations. The use of controller for thermal control system of fuel and engine oil depending on the software obtained by approximating the graphical solution of the system allow to maintain the temperature with a error of ± 3 ° C for fuel and engine oil, which corresponds to the values ​​obtained experime

Aim of this research was quantitative detection of Increase and decrease in copy number of CaMV-35S promoter and Nos terminator in genetic modified tomato by using Real-time PCR. Twenty four samples of genetic modified tomato seeds were isolated from 84 tomato samples collected from Iraqi markets during the period from December 2016 to January 2017. The experiences were conducted in the Institute of Genetic Engineering, University of Baghdad. Go Taq®qPCR master mix kit supplied by USA Promega Company, three specific primers to CaMV-35S promoter, T-Nos terminator and β-actin housekeeping gene supplied by Canadian Alpha Company were used. To quantitative detection of increase and

The experiments were conducted on farm of the Agriculture College, University of Basrah, in a silty clay soil with moisture content of 16%. The tractor used in this research was (CASE JX75T) tractor for studying and evaluating its agricultural performance. The parameters of study include three level of tillage depths (10, 15 and 20 cm) and four forward speeds (G=1.9, G= 2.44, G3= 3.25 and G= 4.33 km/h) by using randomized complete block design (RCBD) with a split plots. The results showed that the increase in forward speed from (1.9 - 4.33km/hr) led to a significant increased in drawbar pull and wheel slippage by the following percentages (33.33%-15.78%).The obtained results for

Two experiments were carried out, the first at the College of Agriculture - University of Baghdad during spring season 2017 Everest cv. class (Elite) was used to study the effect of foliar application of calcium and magnesium and addition of humic acid to the soil on potato growth and yield, The layout of the experiment was factorial within RCBD design using three replicates. Calcium and Magnesium sprayed with concentrations (0, 500, 1000 mg.L^-1), while the  humic acid was added to the soil with (0, 0.75 gm.m²),  The second experiment included  storage of tubers produced from the spring season, with to study the effect of field treatments  on improving the storability of

The objective of this research was to identify the roles played by the specialized beekeepers associations in the central and southern governorates of Iraq in the following fields .Data management, extension, equipment, coordination, marketing, encourage of innovation , as well as to identify the problems facing the specialized associations ,from sample of 122 respondents ( All members of the board of the specialized associations of 40 respondents,and random sample of 18% of beekeepers belonging to these associations of 457 beekeepers and 82 respondents). The results of the research concluded that the percentage of specialized associations for th

This study was carried out at the experimental field, College of Agriculture, University of Duhok, during 2013- 2015, using six genotypes of pea. The objective of this experiment to investigate heterosis and several genetic parameters by using full diallel cross. The results revealed that general combining ability were significant in all studied traits except No. of grain pod^-1, also special combining ability and reciprocal were significant in all traits at probability 5%.

Fifteen Awassi male lambs were randomly distributed to five groups and fed ad libitum roughages: hydroponics barley and/or green alfalfa with different levels, 0: 100, 25: 75, 50: 50, 75: 25 and 100: 0% as roughage experimental diets T₁, T₂, T₃, T₄, T₅ to study in-vitro and apparent digestibility, rumen fermentations and total rumen bacteria using completely randomized design. Initial weight of replicates were 21.73 kg ± 4.17 kg and 3-4 months old. Concentrate fed at 3% of live body weight based on dry matter basis. Results showed: No differences for in-vitro digestibility, while there were significant increases (p<0.05) for total digestible nut

This experiment was carried out  at a private palm farm located in Great Musayib enacted / Babylon province on Barhi dates palm trees Phoenix dactylifera L. cultivar to determine the effect of spraying of chitosan and addition of both organic fertilizers and amino acids and seaweed extract in some vegetative traits (number of leaves,  the circumference of the trunk and offshoots number)  and leaves content (chlorophyll , dry weight and Proline) by spraying the chitosan at concentrations of 5 ml. l^-1  and 10 ml.L ^-1 and the addition of both organic fertilizer and amino acids and seaweed extract at concentrations (1, 2) kg.tree ^-1 and (3 , 6) ml . L ^{- 1} and (4

Amphibians cutaneous glands spread over the skin which contain different bioactive substances. So, the aim of this study is to study the effects of frog skin secretions on parasites and pathogenic bacterial isolates. Five adult frogs (Rana ridibunda) from AL-Jadria streams in Baghdad used in this study. The frogs were stimulated by single intraperitoneal injection of norepinephrine-HCl (40ng/gm) body weight. Frogs skin glands secreted bioactive substances then washed .The washing solution was centrifuged, lyophilized and re-suspended and sterilized. The  result  of  the study  revealed that the concentration (500mg/ml) inhibited bacterial growth so as Staphylococcus aureus,

Iran is a part of the secondary center of origin of melons, and therefore, there is a wealth of genetic variation of these species in this country. In this work, 14 morphological traits were studied in order to select the most discriminating traits. Significant difference among accessions was revealed for almost quantitative traits. KMO coefficient obtained was 0.723 that is explaining of appropriation of this test. The results of the principal component analysis (PCA) for the morphological traits indicated that the first three PCs explained 53.46% of the total variation. Principal component analysis results (PCA) for morphometric data indicated that samples of Semnan and Khorasan showed high degree of  variation and were hi

A comparison was conducted  between volatile oil of the plant parts leaves and stems of Pelargonium graveolens as antioxidant by using the compound  DPPH as a free radical. This study aimed at isolation and diagnosis the active compounds of the volatile oil  .The volatile oil was extracted by using the water distillation Clevenger .Volatile oils were detected by using  the liquid gas chromatography. The results showed that 100 g of leaves and steams produced a volatile oil estimated by 5% and 2.3% respectively. The study has shown that volatile oil of leaves contains 12compounds and the highest was Terpineol reaching

The present study included the collection of 144 samples from various clinical and environmental  sources  to investigate the presence of P.aeruginosa. 45 isolates were identified as P.aeruginosa  based on morphological and biochemical tests in addition to molecular diagnostics  used of 16S rRNA. This diagnosis proved that all isolates belong to P. aeruginosa. All isolates selected susceptibility toward 11 antibiotics using disc diffusion method. The results showed a high resistance among isolates against Tetracycline, Cefixim, Cefotaxim, Amoxicillin,   Erythromycin, Methicillin,

This experiment was carried out at greenhouse in Centre of Biotechnology Researches/ University of Nahrin using soil clay loam texture, soil was in pots closed to study the effect of different salinity levels on detection TaSOS4 gene and yield traits in some bread wheat genotypes (Triticum aestivum L.).Wheat seeds were planted in Nov. 2016 with ten seeds in each pot. a factorial experiments within Randomized Complete Block Design with three replications was used and Four levels of salinity (4, 8 , 12 and 16 ds.m^-1) with four genotypes (G1:Iraq, G2: 2H ,G3: 3H and G4: Hussein). The result showed non-significant differences among genotypes under first salinity levels (4 ds.m^-1

A field experiment was carried out at the fields of Al- Husseiniya District, Taliaa Township, Babylon Governorate, to study the response of the Jerusalem artichoke to foliar application of  micro nutrients nano-fertilizes of Nano Iron, Zinc, Copper ,and Manganese foliar applied at 25, 50, 75 and 100 g nano-fertilizer 100 L^-1 water, and 1 kg naonfertilizers ha^-1(as recommended) dissolved in  400 liters of solution ha^-1.The experiment included single , di, tri and tetra combinations, as well as a tetra combination of a traditional source, in addition to control(distil water only) using RCBD with 3 replicates . Growth parameters tested were chlorophyll SPAD, dry matter yield of vegetative,

The present study deals with the estimation of gill surface area for orange spotted grouper (Epinephelus coioides) collected from Iraqi marine water, north west Arabian Gulf. In the laboratory, fishes were separated in to five different length groups ranging from 200-325 mm and weight range of 108.8-582.2g. Absolute and relative total gill surface area (GSA) were estimated for 33 fish from different lengths. Statistical analysis of results revealed that there were significant differences (P<0.05) between total length of gill filaments (TFL) in different length groups, while there were no significant differences (P>0.05) between secondary lamellae (Bl) in different length groups. St

The aims of this study were to detection of dominant Salmonella species that caused poultry infection Salahaddin province , and evaluation  local prepared and manufactured serological kits that used in diagnosis of poultry salmonellosis, for this purpose 100 diarrheatic  hen checked by culture methods, PCR, ELISA, Whole blood agglutination test and Slide agglutination test. The results showed that Salmonella isolation from hen  in rate 34% , intestine is most suitable site for Salmonella isolation and Salmonella. typhimurium is most dominant spp. The sensitivity of ELISA test was 76.4%, while for other used tests were 100%. The specificity of ELISA test, Whole blood agglutination test,&nbs

This study was aimed to isolate, identify and characterize the bacterial pathogens causing calf diarrhea in Baghdad province and study it's susceptibility toward different antibiotics. The study was conducted on 105 faecal samples collected directly from the rectum of diarrhoeic calves and brought to the laboratory for bacteriological examination. Isolation and identification of the microorganisms were confirmed on the basis of their, staining, cultural, morphology and biochemical tests. Furthermore, antibacterial test was study for different clinical isolates of pathogenic bacteria toward varying concentration of antibiotics by disc diffusion technique. samples

This study was carried out in order to isolate and diagnosis of common bacteria from bovine mastitis in Baghdad city which have the ability to produce biofilm and detect its molecular composition. Twenty five milk samples were collected from different regions in Baghdad city from udders of cows suffering from clinical and subclinical mastitis.Then cultured on blood agar. Gram staining was done to differentiate between bacteria gram positive which cultured on Mannitol Salt Agar and Nutrient Agar while gram negative bacteria were cultured on MacConkey agar and Eosin Methylene Blue agar. All bacterial isolates were subjected to different biochemical tests, API 20 E System, API Staph System and RapID^TMONE System kit to con

A project to develop new maize (Zea mays L.) inbreds from inbred populations, crossed, and tested for performance. The project elapsed six seasons, 3 spring and 3 full plantings during 2015-2017. Newly developed inbreds showed significant grain yield increase as compared to the cross of its progenitor inbred. An increase of 2 t ha^-1 was obtained with one of the new inbreds. The crosses obtained were evaluated in field trials with a registered hybrid (60×21). Method of selection counts on a unique plant trait in the inbred population, such as longer ear, thicker ear diameter, stay-green leaves, large kernel, and so on. The merit obtained due to hybrid vigor could be attributed to parental inbreds that have hig

Afield experiment was conducted during in fall season 21 /7/2016 to screen  100 top crosses ,which is  represented   S3 generation, derived from  popcorn population AGR-2,  After one cycle of reciprocal recurrent selection (RRS). as The genotypes AGR-2 and Suror used  vtesters and evaluated with top crosses with control variexy. The experiment was carried out using 10x10 partial balance lattice design with two replications. Three statistical criteria for screening were used, First, standard error, the second duplicated standard error value and the third standard division value. All the values  statistical criteria were added to total mean. The results were  showed  a significant d

This study aim was  antioxidant production from local fungal isolate, Twenty six purified isolates of Aspergillus sp. were obtained by using different sources ( soil, Spoiled fruits, wheat, barley, maize and Spoiled bread). The isolates were examined for their aflatoxins production, Non-aflatoxins producing isolates were used as phenolic compounds producer. The highest phenolic compounds (45.3 µg/ml) was obtained from  Aspergillus sp.BP1 isolate. This isolate was identified by 5.8 rRNA gene sequecing as Aspergills niger (accuracy of 100%), which was matched with the sequence of Aspergills niger strain YMCHA 55 recorded in Gene bank under the ID:&nbsp

Vital wheat gluten was extracted from broken wheat (Triticum aestivum) and durum wheat (Triticum durum) flour and used to improve the rheological and staling characteristics of bread made using flour from two varieties of barley (IPA 99, IPA 265) which were obtained from the Ministry of Agriculture / Agricultural Research Center. The recent study included seven treatments being, Wheat flour was used as control treatment in bread making (T1), and barley class   IPA99 flour (T2) , IPA 265  flour (T3), IPA99 flour with  durum wheat gluten (T4), , class IPA 265, flour with durum wheat gluten (T5),   IPA99 flour with broken wheat gluten(T6), IPA 265 flour with broken wheat gluten(T7). The bread samples we

This study was conducted  at the Ruminants Researches Station (25 km west of Baghdad) as well as the Directorate for Agricultural Researches / Ministry of Agriculture, as from the  Reproductive laboratory at the College of Agriculture/University of  Baghdad  from 01/03/2015 to 15 / 09/2015 on sample  94 awassi dams . In order to study effect infection on production traits to growth traits , The percentage of healthy dams was 70.21%, while the proportion of ewes was 29.79% and the differences between the two were highly significant (P <0.01) The results of the current study showed that the percentage distribution of the difference between the parasite type of the sheep infected with the

To study the effect of mineral fertilizers (NPK), Organic  and Bio-fertilizers on grain yield of bread wheat(Triticum aestivum L.). A field experiment was conducted during 2014-2015, 2015-2016 at the experimental farm of Field Crop Department, College of Agriculture, University of Baghdad ,Iraq. A split plot arrangement within RCBD, using four replications for two varieties at the main plots  and 14 fertilizer treatments at the sub plots:T₀ (control) ,T₁ (mineral fertilizer as recommended), T₂ (bio-fertilizer), T₃ (organic fertilizer), T₄ (bio+ organic fertilizers), T₅(mineral fertilizer +bio- fertilizer), T₆(50% mineral fertilizer + b

Field experiment was conducted at field of Fallujah- Anbar Governorate/ Iraq, during the two seasons of 2017. Seeds of eight maize inbred lines were cultivated, and line × tester method was used to produce 16 crosses. The seeds of parents were cultivated and crosses in the fall season of 2017 in a randomized complete block design with three replicates. The objectives of the study were to estimate gene action, hybrid vigour, and the effects of general and specific combination abilities of the inbred lines  and to evaluate test cross performance of the hybrids for grain yields and yield related traits. There were significant differences between parents and their crosses for all studied traits. The results showed that crosses

The certain crop cultivars could be adapted that characterized their performance via given trait over given environments. Thus this adaptation could be investigate by many methods. Therefore, ternary plot method advocated to assess the adaptation of ten caraway cultivars using seed yield (Mg ha^-1) over two environments viz. Fallujah and Ramadi for two successive seasons (2011/2012 and 2012/2013). This method was fortified with AMMI Biplot technique. PAST software was adopted to construct triangle graph. Cropstat7.2 software was also applied to extract cultivars own biplots. Results from ternary plot showed that G7 was the both most adaptable cultivars over most circumstances, G3 irresponded because it located on point

Bradyrhizbium sp. was isolated from 40 legumes roots and soil samples collected from various agricultural areas of Iraq by growing them on yeast extract mannitol agar (YEMA) and identification based on culture and microscopic characteristics, biochemical and physiological test and PCR technology.  A split plot experiment was carried out to study the effect of biofertilizer prepared from this isolate on the growth and yield of mungbean plant in sterile and non-sterile soil. The results showed a significant increases in all studied growth and yield traits due to biofertilization. The increase percentage  for the biofertilized treatments was 159.3

Using modern technology is one of the most important means that affect the productivity of wheat, the achievement of profits, the increase of agricultural production, and the progress towards self-sufficiency and food serenity. Therefore, the research aims to study the effects of the use of modern technological packages on the profits efficiency of wheat farms in Iraq for the season 2016-2017 in the provinces of  (Wasit, Babylon and Diwaniyah (, through estimation a stochastic profit frontier  function and inefficiency function. The results showed that the values of the parameters were significant and positive for the durum income and were significant and negative for the parameters of the production costs (mechanizatio

This study  was aimed to measure the production efficiency, cost efficiency, profit-maximizing production level, and net income at actual, cost-minimizing, and profit-maximizing production, as well as to calculate the minimum priced accepted by cucumber producers to supply their products, estimate supply function for cucumber producers, estimate the marketing efficiency of cucumber grown in greenhouses. A random sample of 45 cucumber growers from Dhi Qar governorate, Al-Shatra district/ Iraq during the agricultural season 2017 was selected. Due to its harmony with economic and statistical logic, cubic formula was used to estimate the cost function. Results showed that the optimal production of cucumber was 59.54 tons, and th