Ascaridiosis is a very important parasitic disease of birds, it is caused by Ascaridia. This study was conducted to identify the Ascaridia species by microscopic and molecular assay in Baghdad city. One hundred and sixty fecal samples were collected from domestic pigeons during the period from 1/1/ 2019 to 31/3/ 2019. Results showed that the rate of infection for Ascaridia spp. 15.62% by microscopic examination. Significant difference was observed in infection rates between males and females pigeons. Fifty samples randomly selected and subjected to molecular diagnosis of Ascaridia spp.. Molecular examination results, the total infection rate showed 16%(8/50). The eight p
... Show MoreThis study was aimed to demonstrate the seroprevalence and molecular detection of Toxoplasma gondii among ewes and horses as well as to determine the risk factors for infection in Duhok province. Sera and blood samples from 700 ewes and 62 horses were collected. Sera were examined by indirect ELISA for detection of anti-T. gondii IgM antibodies and in molecular study, DNA was extracted, then by PCR, B1 gene was amplified and the product visualized and sent off for sequencing. Serologically, the prevalence of toxoplasmosis was 17.7 (11/62) and 28.9 (202/700) in horses and ewes by ELISA respectively. Present of cats on the farm was significantly associated with T. gon
... Show MoreThis study was aimed to produce AuNPs biologically using Klebsiella pneumoniae and study their synergistic effect with some antibiotics.Technologies of nanoparticles are quick and are employed in many applications in biomedicine. The potential of metallic nanoparticle as an anti-microbial agent is greatly investigated which considered as an alternative method to reduce the challenges of multi-drug resistance microbes. The present study discusses the novel approach to synthesize nanoparticles involving eco-friendly synthesis of gold nanoparticles using Klebsiella pneumoniae and study their effect as antimicrobial spectrum .Also study synergism effect of gold nanoparticles with an
... Show MoreThis study was aimed to purify, characteristic, and fibrinolytic activity of staphylokinase (SAK), is an enzyme activates plasminogen to form plasmin, which digest fibrin clots that cause thrombosis clot. Staphylokinase was purified from local isolate Staphylococcus aureus GH38 by ammonium sulfate precipitation at 70% saturation followed by ion exchange chromatography (CM-Cellulose) with a purification fold 2.73, and 1.53, and recovery 72.1, and 33.11% in wash and elution steps respectively. The partially purified enzyme was high activity at 40°C with pH 7 and the enzyme retained 100% of its activity at 35°C with pH 7. The activity of an enzyme increas
... Show MoreThree identically designed microbial fuel cell-constructed wetland (MFC-CW) systems were constructed and setup in this study for simultaneous biotreatment of petroleum refinery wastewater (PRW) and bioelectricity generation. MFC-CW1 and MFC-CW2 were planted with Canna indica, and Phragmites australis, respectively. MFC-CW3 was unplanted and considered as the control. These three systems were operated simultaneously in a batch mode for two cycles to evaluate the effect of PRW biotreatment on the growth and development of the selected plants and the potential of generated bioelectricity as well. The operation period for each cycle was 8 days. Results demonstrated that maximum removal efficiency of t
... Show MoreThis study was aimed to investigate the ability of N.oleander to remove Cadmium (Cd) from wastewater. A prolonged toxicity test was performed in a single exposure and run for 65 days with various concentrations of Cd. Plants were grown in sand medium and irrigated with simulated wastewater contaminated with Cd, using different concentrations (0, 10, 25, 50, 75 mg/L), which were chosen based on previous preliminary test. The results of physical observation of the plants didn’t show any withering symptoms. The Cd concentration in plants increased, while in water decreased. The results of plant analysis showed that Cd concentration in plant shoots (stems and leaves) was higher than that in roots for almost all exposure do
... Show MoreThis study was designed to determine ZmHKT1;5 gene expression in relatively salt tolerant using four maize genotypes (Alfajr and Almaha and medium-tolerant ones T1 and T6) within cellular level. Three salinity levels (2, 8, and 16 ds / m . Were used immature seeds were surface sterilized and embryos were dissected under full sterilization conditions. Callus was initiated from immature embryos of the above four genotypes on Skoog and Murashige (MS) medium supplemented with the required growth regulators (3 mg/L 2,4-D +0.5 mg/L kinetin) and recultured for three times and transferred to the stress medium containing NaCl.
This experiment aimed for evaluating the stress and financial losses resulting from transporting broiler chickens from farms to slaughterhouses under harsh Iraqi conditions. Data were collected from 18 farms, where 42,564 broiler chickens were transported to slaughterhouses. Depending on the time of journey, the marketed birds were divided into three groups: short-time (T1), medium-time (T2), and long-time (T3) that lasted 3, 6, and 9 hours, respectively. The total percentage of mortalities resulting from transporting broiler was 0.46%, while the percentage of lost weight was 5.12%, thus the percentage of total losses resulting from transportation reached 5.58%. The overall percentage of mortalities and the percentage
... Show MoreAn experiment was conducted to evaluate fertility, hatchability, egg weight and egg production traits from three different lines of local quails. A total number of 945 eggs (334, 321 and 290 for desert, brown and white, respectively), from 20 weeks of age were used in the study. White quail significantly (P≤0.05) produce higher egg weight (12.79 ± 0.08 g), chicks' weight (8.89± 0.02 g), percentage of mortality (2.34 ± 0.70 %) and Hen-day egg production (79.33 ± 0.98 %) comparing with other local quails. While local brown quail have significantly (P≤0.05) higher percentages of fertility and hatchability which averaged 79.11 and 86.80%, respectively. The higher total number of egg and low m
... Show MoreThis study was conducted at the poultry farm -Animal Production Department/ College of Agricultural Engineering Sciences- University of Baghdad, for the period from 2018/8/17 to 2018/9/28, in order to study the effect of adding inorganic and organic selenium to the broiler diets reared at high temperatures in the physiological performance. A 420 un-sexed chicks of breed (Ross 308) were used in the experiment as a one-day old and with weight rate of 36.4g. The chicks were distributed randomly and equally on 7 treatments, each one included 3 replicates at 20 chicks/replicate. The experiment included the following treatments : T1,T2, and T3 as organic selenium treatments which are selenomethionin with a concentrati
... Show MoreThis study aimed to determine antibiotic resistance profiles and the distribution of Integron I, Integron II, and genes among uropathogenic Klebsiella pneumoniae isolated from patients suffering from urinary tract infection. The results revealed that one hundred and thirty-seven (91%) out of 150 clinical specimens were Klebsiella pneumoniae. The isolates (137 isolates) were given a higher percentage of resistance against Piperacillin (94.89%)and Rifampicin (93.43%) and the lowest resistance was with Meropenem (40.87%). According to PCR amplification
In order to conserve the ecosystems and biodiversity of wetland areas, it is necessary to monitor the degradation of these areas. Currently, Al Razzazah lake and its surrounding areas have degradation significantly due to its low water level, which has negatively affected its biodiversity. Hence, this research aims to propose a method to model the monitoring of spatio-temporal changes in that lake and its surrounding areas with an area estimated at 4660 km² between (1998 – 2018) using Remote Sensing and Geographic Information System (GIS) techniques. After conducting the supervised classification by the method of Support Vector Machine (SVM) for all satellite images, we extracted thematic maps, which contain five c
... Show MoreThe aim of this study was to investigate the presence of Mycoplasma synoviae in broiler and layer chickens infected with respiratory signs. A total of 80 samples were collected randomly from layer and broiler chickens with respiratory signs in Baghdad from the period between January to May 2017 from the trachea, Lung, Air sac, oviduct, tracheal swabs, conjunctiva swabs, choanal swabs, and nasal swabs and cultured in PPLO medium with supplements, then positive culture subjected to DNA extracted and Polymerase Chain Reaction assay (PCR) to detect Mycoplasma as a genus and Mycoplasma spp by using specific primers targeting16S rRNA gene. The results of culture revealed that the total rate of Mycoplasma<
... Show MoreThis study was aimed to investigate the effect of phytase as a feed additive on growth performance and survival rate against Saprolegnia spp.in common carp, Cyprinus carpio L. A total of 120 C. Carpio (initial mass 20.05-20.35g) were randomly stocked into six treatments in duplicate (10 fish/tank) as follows: T1, T2, and T3 were fed the basal diet supplemented with phytase at 1000, 2000, and 4000 IU/Kg diet respectively, while T4 were fed on basal diet plus β-glugan at a concentration of 8.5 g/kg as well as the control group was fed basal diet without any addition of phytase. Results showed after 60 days of feeding period, that the addition of phytase enzyme was significantly (p<0.05) affected on da
... Show MoreThis study was conducted to examine the possible effects of Ag-NPs synthesized using the olive leaf extract on histopathology and cytogenetic effect in mice. A total of thirty albino mice aged two months were divided into five treatment groups as follows: Group 1: served as control was administrated orally with 0.3 mg/kg bw of normal saline; Group 2 and 3 were administrated orally with varying doses of Ag-NPs synthesized on olive leaf extract (10 and 100 mg/kg bw, respectively) for 30 days; Group 4 and 5 were administrated orally with olive leaf crude extract (300 and 1000 mg/kg bw, respectively) for 30 days.
... Show MoreDietary replacement of soybean meal by toasted sunflower meal in Clarias gariepinus diet was investigated in a 56-day feeding trial using growth, body composition, digestibility, haematology and liver histology as indices of assessment. Sunflower seed meal was toasted at 2040C for 5, 10 and 15 minutes. Control diet consist of soybean meal which was replaced by differently toasted sunflower seed meal at a rate 15, 30 and 45% to produce nine 40% crude protein, 18kj/kg test diets. Triplicate groups of fish in 70-litre capacity aerated rectangular plastic tanks were assigned to each dietary treatment at a stocking rate of 15 fingerlings (4.97±0.42g average weight) per tank in a completely randomized design. Fish
... Show MoreIn the last decade, tilapia fish species distributed in the Iraqi inland waters. Three species; Nile tilapia Oreochromis niloticus (Linnaeus, 1758), Blue tilapia Oreochromis aureus (Steindachner, 1864) and Redbelly tilapia Coptedon zillii (Gervais, 1848) inhibiting Shatt Al-Arab River. They belong to family Cichlidae. They are very similar to differentiate among them using biometry. So, genetic markers used for species discrimination. Randomly amplified polymorphic DNA (RAPD) protocol used to examine genetic variation and to generate DNA fingerprints of tilapia fish species in Shatt Al-Arab River. Sixty-two specimens of tilapia fish collected from Shatt Al-Arab
... Show MoreThis experiment was conducted and aimed to reducing mortality and losing the weight of birds transported to the slaughter house through reducing or Transport stress removal and thus to reduce financial losses. 150 birds used almost equal weights that were divided into three treatment groups and by five replicates for each treatment is control treatment (T1) without giving anything and treatment with salicylic acid concentration 1 g / liter of drinking water (T2) and treatment with ascorbic acid (T3) concentration 0.5 g / Liter of drinking water provided 24 hours before its transport. The transport process resulted in a highly significant decrease (P ≤ 0.01) in body weight in treatment T1 as it reached 4.66%, while it reached 1.
... Show MoreThis experiment was conducted to investigate the effect of different levels (5g/kg ,10 g/kg or litter) of chamomile flower in feed and water on performance and gut health of broiler chickens under E. coli challenge. A total of 480-day-old Ross 308 broilers were randomly assigned to 12 treatments each replicated 4 times with 10 birds. Oral inoculation with E. coli was on days 8 and 9 of birds age. At day 10 and 35, the interaction (p<0.05) between the experimental factors decreased the feed intake in the challenged birds supplemented with chamomile. At day 10, unchallenged birds were heavier (p<0.05) than the challenged groups. Up to 35 days, birds on antibiotics (zinc bacitracin 360g
... Show MoreThis study was aimed to estimate the influence of Conocarpus erectus L. residues, and some micronutrients on growth and production of potato. This research was conducted at one of the fields of the College of Agricultural Engineering Sciences - University of Baghdad. The experiment was implemented using factorial arrangement (4X3X3) within randomized complete block design with three replicates. Conocarpus fertilizer was represented the first factor with three levels (7.5, 15, 30 ton.ha-1), which symbolized (C2, C3, C4). Mineral fertilizer as recommended dose as a control, which symbolized (C1). The second factor was foliar spraying with three levels of iron (0, 100, 2
... Show MoreThis study was aimed to determine the residual concentrations of pesticides used in greenhouse cucumber fields and their effects on the farmers that spray it. Sixteen active cucumber greenhouse fields with 96 people (75 sprayers and 21 volunteers as control) randomly had been selected. In all selected greenhouses, abamectin, thiamethoxam, pyridabin, and spirodiclofen pesticides were used, their cucumber with leaves and groundwater collected in December 2017, March, June, and September 2018 with workers blood samples, were immediately brought to the laboratory. Results showed the occurrence of residual pesticides in the plant and groundwater samples with a highest
... Show MoreThe objective of this study was to evaluate the effects of yeast (Saccharomyces cereviceae) as additive for barley silage preparation at different ensiling times. Small scale silages were prepared from barley forage and four yeast levels were evaluated (0 (control), 5, 10 and 15 g/kg FM). Silos were opened at 6, 12 and 24 days. Three silage-replicates were prepared for each yeast level × ensiling time combination. Silage quality in terms of CP and NDF and ADF concentration appeared to improve when yeast was added at 5 and 10 g/kg, compared with the control, but the quality decreased again when 15 g yeast/kg FM were aded to the silage. However, gas production, metabolisable energy and organic matter digestibility linearl
... Show MoreThis study was aimed to asses seed yield performances of 16 rapeseed genotypes in randomized complete block designs (RCBD) with three replications at four Agricultural Research Stations of cold and mid-cold regions over two years in Iran (2015-2017). GGE biplot analysis indicated that the first two components explained 83% of seed yield variations. Genotype, location and their interaction explained 18%, 52% and 30%of the total GE variation, respectively. In this research, a graphically represented GGE biplot analysis enabled selection of stable and high-yielding genotypes for all investigated locations, as well as genotypes with specific adaptability. The GGE biplot analysis was adequate in explaining GE in
... Show MoreAn experiment was conducted in a greenhouse - research station B - College of Agricultural Engineering Sciences, University of Baghdad, during the fall season of 2018 with the aim of propagating and initially studying the field performance of 18 and 20 potential potato lines derived from Rivera and Arizona cv. after in vitro exposure of nodal segments to different dosages of gamma rays (0, 10, 20, and 30 Gray) and EMS (0, 10, 20, and 30 mM). Each control cultivar and their derived lines were independently cultured in plastic bags according to the RCBD, with three replications. The results showed that the highest plant height and number of leaves were obtained from Arizona derived lines which gave 60.11 cm and 25.30 leave
... Show MoreThe current experiment was designed to investigate the effect of zeranol implantation on lambs and goats’ kids raised under commercial conditions. Ten male Awassi lambs (AL) (4-5 months old) with an average body weight of 33.7 ± 0.5 kg and 10 male black goat kids (GK) (3-4 months old) with an average body weight of 18.8 ± 0.7 kg were used in the experiment. The experiment was conducted in Berebuhar village close to Duhok city in May the 15th, 2019The animals from each group were randomly divided into two subgroups, the first group was the control group and the other group was implanted with 24 mg zeranol. The animals were grown for 43 days and then slaughtered. Growth rate, average daily gain and carcass characteri
... Show MoreThe aim of this research was to study the effect of feeding of goat kids on Panicum Mombasa grass on productive traits, carcass characteristics and meat production. 25 of local cross breed male goat kids; average weight 18.3 kg were divided randomly to five treatments, the first one T1 was fed with wheat straw, T2 green alfalfa, T3 green Panicum Mombasa, T4 alfalfa hay and T5 Panicum Mombasa hay. Animals reared in single cages for two weeks as a preliminary period, the experiment period lasted for 70 days. Concentrate diet provided with 3% of body weight, roughage feeds provided freely. At the end of the experiment, three anima
... Show MoreThe current study was conducted during the period from July 2018 to April 2019. One hundred and forty samples from different regions of Baghdad city, Iraq, were collected from 50 calves, 40 children, and 50 water supplies for diagnosis of Giardia intestinalis. The prevalence of Giardia intestinalis in calves, children, and water by microscopic examination by using sucrose flotation, 30%, 27.5%, and 16% respectively. Twelve positive PCR products were sequenced and deposited in the Genebank database for the first time in Iraq, phylogenic analysis demonstrated that 5 sequences belong to G.intestinalis for human(MN244831, MH925331, MN244829, MN244828, MN244830), 3 sequences for calves (MN244827,
... Show MoreThe element-substituted calcium Deficient hydroxyapatite samples were synthesized using liquid-phase precipitation method. Different element ions (Mg+2, Zn+2, and Cu+2) were added at constant ratio (5% wt.). The phase composition of the element-substituted calcium hydroxyapatite samples was investigated by using X-ray diffraction. A comparison with known characteristics of the synthetic ion-substituted forms of hydroxyapatite was conducted. It was determined, that the investigated materials are single-phase solid solutions of the element-substituted hydroxyapatite. By the methods of IR spectroscopy and Raman scattering (RS) it was discovered the dependence of incorporation of the carbonate anion t
... Show MoreThe current study was designed to explore the association between the pigments production and biofilm construction in local Pseudomonas aeruginosa isolates. Out of 143 patients suffering from burns, urinary tract infections (UTI), respiratory tract infections and cystic fibrosis obtained from previous study by Mahmood (2015), twenty two isolates (15.38%) were identified from (11) hospitals in Iraq, splitted into three provinces, Baghdad, Al-Anbar and Karbala for the duration of June 2017 to April 2018. Characterization was carried out by using microscopical, morphological and biochemical methods which showed that all these isolate
... Show MoreThis study was aimed to evaluate the ability of local oyster mushroom(Pleurotus ostreatus; ID: MF065715.1; cultivated until maturation in growth medium containing cumin extract) to modulate the genotoxicity induced by cyclophosphamide (CP) in bone marrow cells of mice. In vivo genotoxicity was assessed by quantifying the incidence of micronucleated polychromatic erythrocytes in the bone marrow cells of mice that were administered different doses of P.ostreatus extract (150, 200,250, and 300 mg/kg/day( respectively, individually, and in combination with CP (40 mg/kg body weight) according to the following three experimental protocols (pre 2h, post 2h, and concomitant treatment for 14 day
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