Objective: To evaluate the therapeutic activity of probiotics mixture of Lactobacillus plantarum and Lactobacillus acidophilus towards Cryptosporidium infection in experimentally infected mice. Oocysts of Cryptosporidium were separated from the stool of humans to infect mice. Methods: Forty male albino mice were split equally into four groups, every group contained 10 mice, the group I (early treated group), were treated from the 1st day from infection to the 11th post-infection, group II (late treated group), were treated from the 4th day from infection to the 15th post-infection, and group (III) (untreated group), were mice considered as a positive control group. Results: It was showed that daily application of a mixture of L. plantarum with L. acidophilus could reduce the parasitic infection in mice as compared with the untreated group, and it was confirmed that the using of these probiotics in the early treated group was more efficient than the using of these probiotics in the late treated group. Conclusion: A mixture of L. plantarum and L. acidophilus are good therapeutic agents for cryptosporidial infection
Probiotic bacteria using in commercially produced foods are at most members of the genera Lactobacillus and Bifidobacterium. This research was aimed to study the antimicrobial effect of lactobacillus bacteria as probiotic against some pathogenic bacteria, strains of lactobacillus bacteria were isolated from two types of yoghurt in Baquba city, Iraq . Total of 60 yoghurt samples from 2 origins (30 locally and 30 commercial produced yoghurt) were used to isolate probiotic bacteria which were identified phenotypically and biochemically. 30 (100%) of locally produced yoghurt samples include with probiotic bacteria while 12 (40%) commercially types of yoghurt samples were contained with probiotic, the method of modi
... Show MoreThe aims of study is to detect the inhibitory effect of Saccharomyces boulardii and Lactobacillus acidophilus on Escherichia coli that has been isolated from recurrent urinary tract infection in women. The sensitivity of E.coli isolates to antibiotics had been studied and the most resistant E.coli isolate to antibiotics had been studied .The cup assay was used on nutrient agar and Muller-Hinton agar to detect the inhibitory activity for each S.boulardii yeast grown on YEGP media and L.acidophilus grown on MRS media in which the result showed a high inhibition activity for each of them .Also in this study the adhesion property of E.coli had been evaluated in the presence of S.boulardii at concentration of 1×109 and L.acidophilus at conc
... Show MoreAim: To evaluation the effect of Lactobacillus acidophilus on Enterohemorrhagic Escherichia coli (EHEC) serotype O157:H7 with detection of some virulence factors. Methods: Two hundred and fifty specimens (stool) from children under five years for both sexes were collected from some hospitals. All isolates were diagnosed according to morphological characteristics, biochemical tests. Monoplex pattern of PCR was used also for detection different genes in (7) Escherichia coli )O157:H7 (isolates; include 16SrRNA, eae, lifA, Stx1,Stx2 that encoded for ribosomal RNA, intimin, lymphocyte inhibitory factor, shiga toxins. Three types of probiotics strains were obtained, Lactobacillus fermentum, Lactobacillus plantarum and Lactobacillus acidophilus (A
... Show Moreهدفت هذه الد ا رسة لاختبار تاثير بعض ظروف النمو على الفاعلية التثبيطية لبكتيريا Lactobacillus delbrueckii و L.fermentum على نمو بكتيريا E.coli وقد دلت نتائج د ا رسة اختبار الفاعلية التثبيطية
للعزلتان البكتيريتان Lactobacillus delbrueckii L.fermentum , ضد العزلة البكترية E.coli المستخدمة في هذه الد ا رسة عند الت ا ركيز المختلفة 100,90,70,50) ( % بان عالق العزلتان البكتيريتان
Lactobacillus delbrueckii و L.fermentum عند التركيز % 100 اعطى اعلى فاعلية تثبيطية ضد
بكتريا E.coli
Diabetes mellitus is a form of metabolic disorder where patients are incapable to organize glucose metabolism. The most common types are Type I and Type II, constituting about 10% and 90% of cases, respectively. The cause of type I diabetes, which usually spreads in children and adolescents, is the disability of the endocrine system to produce insulin. On the other hand, The most common type of diabetes, type II diabetes, is often presented in adults. It is usually presented as a collection of insulin deficiency and insulin resistance. This work was done to estimate the count of microbiota in diabetics to find an appraoch for detection and follow-up treatment. The count of two types of bacteria Lactobac
... Show MoreIn order to screen antifungal activity of lactobacilli that produce bacteriocin against the yeast Candida, ninety-two food and clinical samples were collected. Also, several commercial brands of Lactobacillus probiotics and ready-made isolates of Lactobacillus were used. The isolated lactobacilli were subjected to microscopic, macroscopic, and biochemical tests. Moreover, molecular identification was performed for the best producer isolate. For Candida spp. isolation, seventy- two samples were collected from different clinical sources; in addition, nine of ready-made isolates were also used. All the isolated Candida spp. were subjected to microscopic and macroscopic ex
... Show MoreThe effects of Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus casi
and Lactobacillus rhomunas on the body weight and lipid metabolism on obese rats
were evaluated. In the body weight, the results showed significant deference
between rat groups, the group that consumed 3 probiotic strains showed reduction in
the body weight with ratio 16.63% while the group consumed 2 probiotic strains
showed decreasing with ratio 17.62% compared with control group which increased
in the body weight.In the effeci of probiotics on lipid metabolisim the results
showed significant deference between rat groups, the group that consumed 3
probiotic strains showed decreasing in the cholesterol with ratio 50.7%, triglyce
Background:Lactobacillus fermentum selected as an alternative treatment to prevent or treat urogenital infection based on their probiotics properties and production of bacteriocins.
Objective: The present work was done to study the inhibition activity of L. fermentum cell free supernatant against urogenital pathogens Enterococcus faecium and Enterococcus faecalisinvitro.
Materials and methods: L.fermentum isolates have been collected from vaginal swabs . A supernatant of these isolates has been prepared and its antibacterial activity against 3 isolates of E.faecium and 3 isolates of E.faecalis has been studied.
Results: Different concentrations have been prepared and the most effective one was 1000μg/ml and the most affected is
The aim of this study was to evaluate the possibility of using Lactobacillus cells as a probiotic to treat some vaginal infections. For this purpose, thirty Lactobacillus isolates were collected from vaginal samples subjected to a screening program to investigate their antagonism abilities against four vaginosis species: Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumonia and Proteus spp. Eighteen isolates were selected from the primary screening (agar plug diffusion method) based on their ability to inhibit the growth of 4 indicators which then subjected to a secondary screening program with two methods: detection of bacteriocin activity and
... Show MoreThe Inhbititory effect of cocentrated and non-cocentrated supernatant of the probiotic Lactobacillus salivarius against growth of some potential pathogenic microorganisms which included Pseudomonas eruginosa, Klebsiella spp, Escherichia coli and Candida albicans. The results were diffusion assay revealed that concentrated and non-concentrated supernatant had inhitory effect against pathogenic bacteria with inhibition zone renged between 13-17mm while inhibition effect of concentrated supernatant against C.albicans was inhibition zone 8mm. On the other hand, the effect of these suprnatant against biofilm formation of the tested microorganisms was studied. The result showed that the concentrated supernatant had inhibitory effect on biofil
... Show More