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Effect of Lactobacillus salivarius supernatant against growth and biofilm formation of some pathogenic microorganisms
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The Inhbititory effect of cocentrated and non-cocentrated supernatant of the probiotic Lactobacillus salivarius against growth of some potential pathogenic microorganisms which included Pseudomonas eruginosa, Klebsiella spp, Escherichia coli and Candida albicans. The results were diffusion assay revealed that concentrated and non-concentrated supernatant had inhitory effect against pathogenic bacteria with inhibition zone renged between 13-17mm while inhibition effect of concentrated supernatant against C.albicans was inhibition zone 8mm. On the other hand, the effect of these suprnatant against biofilm formation of the tested microorganisms was studied. The result showed that the concentrated supernatant had inhibitory effect on biofilm formation for all tested microorganisms with percentage (28-29)% against tested bacteria and (23)% against C.albicans.

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Publication Date
Sun Mar 01 2020
Journal Name
Baghdad Science Journal
The Biofilm Inhibitory Potential of Compound Produced from Chlamydomonas reinhardtii Against Pathogenic Microorganisms
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Microalgae present much usefulness for antimicrobial research because of its enormous biodiversity and rapid growth rate. From this study results it is reaveled that Chlamydomonas reinhardtii were isolated from a pond of water in the province of Diwaniyah. The culture supernatants were obtained when extracted with methanol solvent. Antimicrobial activity of extracts was tested for pathogens, and the best inhibition zone obtained was against Candida albicans (32mm), S.aureus (15mm), and to E.coli (9mm). While it showed no effect against both S.epidermidis and Klebsiella spp. Biofilm was formed by all tested isolates with differences in its strength formation. The C. reinhardtii

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Publication Date
Tue Sep 19 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Study of Effect of Lactobacillus gasseri Filtrate on Growth of Some Pathogenic Fungi
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The inhibitory effect concentrated filtrate of local Lactobaciilus

gasseri against Candida albicans and Trichophyton mentagrophytes, T  ruhrum  and Aspergillus  flavus which were isolated from infected cases  in human  beings, tested  using two methods  :well diffu  ion and

Mixed with the mold medium (Sabouraud agar).

The  results  showed  that the concentrated filtrate  of L. gasseri have

inhibitory effect against Cundida ulbicans, the inhibition  zone was 13 mm. As well  as, the concentrated  tiltrate inhibited, considerably Trichophyton   mentagrophytes,

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Publication Date
Thu Jan 20 2011
Journal Name
مجله كليه التربيه الاساسيه
Determination of Antimicrobial Activity of Thuja orientalis Against Some Pathogenic Microorganisms
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The present study was conducted to investigate the antimicrobial activity of the hot water and the hot ethanolic extracts of Thuja orientalis against some pathogenic microorganisms (Staphylococcus aureus, Pseudomonas aeruginosa, Eschericha coli, Proteus mirrablis, Salmonilla typhi, Klebsiella pneumoniae, Bacillus cereus, Bacillus subtilus, Acinobacter, Staphylococcus epidermidis and Candida albicans). Results showed that both the water and alcoholic extracts of this plant exert marked inhibitory effect against all the bacterial isolates and yeast and at different ratio, and it was shown that ethanolic extract was more effective in microbial inhibition than the water extract. Maximum inhibition (16 mm) was recorded against Staphylococcus aur

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Publication Date
Wed Jan 01 2020
Journal Name
Annals Of Tropical Medicine And Public Health
In vitro Scavenging Activity of Rosemary Extract and its Activity Against Some Pathogenic Microorganisms
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Rosemary (Rosmarinus officinalis L.) is one of the most economically important species of the family Lamiaceae. Rosemary extract was examined by applying 2.2-diphenyl-1- picrylhydrazyl (DPPH) radical-scavenging assays. The result proved that rosemary extract had a higher antioxidant activity by absorption at a wavelength of 517a nm by using three different concentrations (0.5, 1.0 and 3) mg/ml which performed the absorbance at (2. 314, 0. 211 and 0.296) nm in comparison with control (21.8, 92.2 and 90) nm respectively. Results obtained using chemical detection of the phytochemicals indicated the presence of flavonoids, phenols, saponins, Steroids and cardiac in rosemary water extract. Water extracts of R. officinalis leaves were inves

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Publication Date
Fri Jul 01 2022
Journal Name
Iraqi Journal Of Science
The effect of D and L- amino Acids on Biofilm Formation in Different Microorganisms
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The D-enantiomers of amino acids have been thought to have relatively insignificant function in biological processes like, D-amino acids are sometimes found in proteins that are not synthesized by ribosomes. While L-amino acids clearly permanent in nature, D-amino acids have previously inapprehensible regulatory roles in the bacterial kingdom, any diverse of bacterial phyla made from these D-amino acids regulate cell wall remodeling in stationary phase and cause biofilm dispersal in aging bacterial communities. Clarification the mechanism by which D-amino acids given cell wall reorganization and biofilm disassembly will undoubtedly discover new paradigms for understanding how extra cytoplasmic processes are regulated as well as lead to d

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Publication Date
Wed Mar 28 2018
Journal Name
Iraqi Journal Of Science
Bacteriocin from Streptococcus salivarius optimized statistically by response surface methodology active against different clinical oral pathogenic Streptococci
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The aim of the present work was to study the possibility of producing an effective bacteriocin from bacteria naturally exists in the mouth. Streptococcus salivarius KA101 was selected among 120 oral isolates collected from healthy people based on its ability to produce an effective bactericidal bacteriocin. Modified tryptic soy agar with 2% yeast extract and 0.1% calcium carbonate was the best medium for the production of bacteriocin with an activity of 40 AU/ml. Response surface methodology (RSM) based on central composite design (CCD) was employed to build a model in order to evaluate the optimum effective factors for bacteriocin production using four factors: yeast extract, CaCO3, pH and incubation time. Based on t

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Publication Date
Fri Jun 24 2022
Journal Name
Iraqi Journal Of Science
A Study of the Effect of Some Growth Conditions on Inhibitory Activity of Lactobacillus delbrueckii and Lactobacillus fermentum to reduce Growth of E.coli
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هدفت هذه الد ا رسة لاختبار تاثير بعض ظروف النمو على الفاعلية التثبيطية لبكتيريا Lactobacillus delbrueckii و L.fermentum على نمو بكتيريا E.coli وقد دلت نتائج د ا رسة اختبار الفاعلية التثبيطية
للعزلتان البكتيريتان Lactobacillus delbrueckii L.fermentum , ضد العزلة البكترية E.coli المستخدمة في هذه الد ا رسة عند الت ا ركيز المختلفة 100,90,70,50) ( % بان عالق العزلتان البكتيريتان
Lactobacillus delbrueckii و L.fermentum عند التركيز % 100 اعطى اعلى فاعلية تثبيطية ضد
بكتريا E.coli

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Publication Date
Thu Dec 29 2016
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Effect of Saccharomyces Cerevisiae and Lactobacillus Plantarum in Growth of Fusarium sp.
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         Saccharomyces cerevisiae filtrate showed inhibitory effect against Fusarium spp. when grow in a liquid medium (Sabouraud) with different concentrations (1, 3, 5) %. The higher inhibitory effect against fungus growth was (24.5) mm at (5%) in PDA medium compared with control (36.5) mm during the seventh day propagation. The filtrate of Lactobacillus plantarum isolate was mixed with the PDA medium ,which showed inhibitory effect against Fusarium spp. The concentrated filtrate( one fold) appcarcd a  higher effect against the same fungus compared with un concentrated filtrate one. Saceharomyces cerevisiae and Lactobacillus plantarum

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Publication Date
Wed Mar 15 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
The Effect of Bifidobacterium breve and Lactobacillus salivarius on the Gene Expression Involved in Patulin Biosynthesis Produced by Penicillium expansum .
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 Penicillium expansum produced the toxin patulin in pome fruits. To evaluate the molecular mechanism by which the treatment of probiotic bacteria Bifidobacterium breve and Lactobacillus salivarius could modulate patulin production, three genes involved in the biosynthesis of patulin were measured using real time-PCR technique. The result of this study found that the supplementation with B. brave and L. salivarius down-regulate the relative expression of 6-methylsalicylic acid synthase (msas), ATP binding cassette transporter (ABC) and putative cytochrome P450 monooxygenases (P450-1) as well. Although, there is no effect of some strains on this expression. However, these finding suggested that these bacteria decreased patulin product

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Publication Date
Sun Sep 06 2015
Journal Name
Baghdad Science Journal
The Inhibitory Effect of Lactobacillus acidophilus and Lactobacillus plantarum against Candida albicans Associated with Denture Stomatitis
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In this study Candida speices was diagnosed in 26 swab samples from patients with denture stomatitis , investigates the antagonism activity of Lactobacillus was investigated against the yeast of Candida albicans in vitro.Results revealed that The inhibition effect of Lactic Acid Bacteria against C.albicans was examined in solid medium, L.plantarum gave higher inhibition average 11mm followed by L.acidophillus with average 9 mm and, L.fermentum , L.casei with averages 7 mm. Whereas the filtrates, the highest inhibition zone were 20 and 16 mm by L. plantarum and L.acidophillus, respectively.

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