: Leucine amion peptidase (LAP)
Arachis hypoaea L. seeds
Purification
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Leucine amino peptidases (LAP; EC 3.4.11.1) constitute a diverse set of exopeptidases
that catalyze the hydrolysis of leucine residues from the amino-terminal of protein or peptide
substrates, (LAP) are present in animals, plants, and microbes. In this study, leucine amino
peptidase was purified partial from Arachis hypogaea seeds by using gel filtration chromatography
Sephadex G-100. The enzyme was purified 3.965 fold with a recovery of 29.4%. Its pH and
temperature optimum were(8.7) and (37oC), respectively. The results show novel properties of
LAP from Arachis hypogaea L. or peanut. The Km value for LAP (77 mM), with V max (1538 m mole
min-1). We recommend a separate isoenzymeof the enzyme (LAP) from Arachis hypogaea on L.
peanut seeds a
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