Summary:
Background: the immune system differentiates between the endogenous and exogenous bodies that inter the body. The break-down of blood-testis barrier results in the production of antisperm antibodies. This may occur in the case of an infection to the prostate, seminal vesicle and epididymes. Antisperm antibodies (ASA) cause sperm agglutination and affect sperm motility, viability and sperm migration in the female reproductive tract. ASA also impair fertilization process. The objective of the present work was to study the effect of prednisolone on sperm motility index (SMI), viability and sperm penetration assay (SPA) in immunologically infertile men.
Materials and methods: The semen and serum samples of 140 infertile men were examined by microagglutination test and slide agglutination test to detect ASA and sperm agglutination. Semen fliud analysis was performed to report sperm motility index (SMI), sperm viability and hypo-osmotic swelling test (HOST). Sperm penetration assay was done to record sperm penetration rate (SPR), sperm decondensation rate (SDR) and sperm penetration index (SPI). Men with positive ASA were treated with prednisolone and considered as treated group Prednisolone was given orally in a dose of 5 mg three times daily for three months. The semen analysis, SMI HOST and SPA were performed before and after treatment with prednisolone. The number of semen samples in the treated group was 144 and in the control fertile group was 80. HOST-SPA positive semen was exposed to antisperm antibodies separation (ASAS) and invitro sperm activation prior to intra-uterine insemination.
Results: The SMI was significantly higher in the post-treated group compared to pretreated group (240 vs. 52.5, P<0.0l). The SPI in the control group was significantly higher than the post and pre-treated groups. The HOST and viability test results were significantly increased in the post-treated group compared to pre-treated group (73.42 vs. 48.56 and 71.36 vs, 50. 74 respectively, P<0.01). The sperm penetration rate, sperm decondensation rate and sperm penetration index were significantly increased in the post-treated vs. pre-treated groups (26.49 vs. 10.84, 10.91 vs. 3.47, P<0.05, 14.45 vs. 4.30, P<0.01 respectively). HOST-SPA positive semen was used for intra-uterine insemination (IUI). The semen was exposed to ASAS and in vitro activation prior to IUI and resulted in 42.86% pregnancy rate per cycle. The pregnancy was confirmed by the observation of gestation sac and viable fetal hear beet 5 weeks following IUI.
Conclusion: It was concluded from the results of the present study that treatment of immunological infertile men with prednisolone improves SMI, sperm viability and SPA results. The application of HOST-SPA positive semen for IUI resulted in successful pregnancy. The authors indicate that these viable spermatozoa with high fertilizable potential could be used for IVF and/or ICSI in immunologically infertile men.
This study aimed to determine the effect of varicocelectomy on sperm parameters, oxidant- antioxidant status and chromatin maturity percent. The current study has been conducted on 154 infertile patients complaining from varicocele and varicocelomized men in addition to 25 fertile men as control. The results revealed significant decrease (P<0.05) in sperm concentration, progressive motile sperm percent, normal sperm morphology percent, GSH, SOD1, CAT levels and chromatin maturity percent and significant increase (P<0.05) in MDA and ROS concentrations in infertile patients with varicocele when compared to fertile men. The results revealed improvement (P<0.05) of sperm parameters quality, GSH, SOD1, CAT, MDA, ROS concentration and chromatin m
... Show More Background: It is evident that there is a lack of clear consensus on the role of luteal phase serum Progesterone (P) level in the prediction of early pregnancy after controlled ovarian hyperstimulation (COH) protocols in assisted reproductive techniques (ART).
Objective: We conducted this study in order to investigate the potential value of luteal phase serum progesterone measurement, in women undergoing ICSI treatment cycles and receiving progesterone supplements, in relation to pregnancy rate.
Patients: A total of 68 women aged 20-40 years undergoing their first intracytoplasmic sperm injection (ICSI) cycles in fertility and I.V.F center of Kamal Al samrai hospital.
Methods: women consecutively treated by ICSI had Estima
The objective of this work was to study the effect of oral administration of Cyperus esculentus (CE) and its alcoholic extract on sperm function parameters in prepubertal mice as a model for human .The animals were divided into three groups each contains 6 animals .Group 1 was treated with 150 mg/ kg body weight /day of crude CE, group 2 was treated with same dose of alcohol extract of CE and group 3 regarded as control throughout six weeks period. The results showed a significant (p> 0.05) increase in the mean of sperm concentration ,sperm motility percent and progressive sperm motility between treated groups and control . There was no differences among groups in the mean of sperm normal morphology and sperm viability . No significa
... Show MoreNon-thermal plasma(Dielectric barrier discharge) has many uses including living tissue sterilization, inactivation of the bacteria, excimer formation, angiogenesis, and surface treatment. This research aim is to use cold plasma as a tool to search the effect of the dielectric barrier discharge system at room temperature on human sperm motility and DNA integrity. This work was performed on 60 human semen samples suffering from low motility; each sample was prepared by centrifugation method, then each semen sample was divided into two sections, the first section is before significant exposure to the plasma system (DBD) and the second section is after treatment with the DBD system at ambient temperature. Before and after exposure to non-the
... Show MoreAim: The aim of this study is to determine the correlation between levels of certain seminal biochemical parameters and serum reproductive hormones, on the one hand, and sperm function tests, on the other hand, in asthenospermic patients. Patients and Methods: Sixty asthenospermic patients and twenty fertile men as a control group were included in this study. Semen samples were collected to perform seminal fluid analysis. Total protein, cholesterol, calcium, creatine kinase, and fructose were measured in the seminal plasma. Blood samples were collected for hormonal assay of serum reproductive hormones: testosterone, prolactin, luteinizing hormone, and follicle-stimulating hormone. Results: The results revealed a significant positive correla
... Show MoreThe objective of this study was to investigate the effect of Royal jelly RJ on morphology and motility of mice sperms. Sperms were collected from the cauda region of the epididymis of each 10 mice from the treatment and control groups. Direct activation techniques and evaluation of sperm morphology were carried out. Dhino microscope was used for sperm measurement. The inspection was carried out in Salamatic laboratory for pathological analysis in 2015.The result revealed that all of the sperm function parameters registered significant activation in the treatment group. There was a significant increase in both the percentage of the sperm motility grade A and the progressive motility (A+B) of the treatment gr
... Show MoreThis study was designed to investigate the effects of licorice extract (Glycyrrhiza glabra L.) addition to semen diluters on ram sperm progressive motility during storage at 5 ˚C for 72 h. Semen was collected from 3 proven Awassi rams. Licorice extract powder was added at levels of 1, 5, 10, 50 and 100 µg per ml. of diluter. Diluter containing no licorice extracts served as control (0).Progressive motility was estimated subjectively after dilution (0h), and at 24, 48, and 72 h of storage. The experiment was replicated 2 times with egg yolk-tris (EYT) diluter and 2 times with yolk- glucose-citrate diluter. Progressive motility increased significantly (p < 0.01) in levels of licorice extract 1, 5, 10, 50 and 100 µg / ml
... Show MoreSummary:
Background: It is well known that the early removal of corpus luteum (CL) in pregnant women results in abortion . Defects in the function of CL lead to deficiency in the secretion of progesterone which adversely affect human embryo implantation .
Aim of the work: l)to determine progesterone concentration in the luteal phase defect (LPD) patients complaining from infertility and 2) to evaluate the clinical value of ovulation induction, sperm intrauterine insemination SIUT and luteal support therapy in the treatment of LPD patients.
Patients & Methods: One hundred and twelve LPD patients were involved in this study. The progesterone concentrations were performed by radioimmunoassay method on cycle day 21. Patients wer
This study was conducted in the Faculty of Agricultural Engineering Sciences - University of Baghdad in the field of sheep and goats in the Department of Animal Production. Effect of different concentrations of bovine albumin on some semen characteristics of frozen rams. Used in this experiment, 3 rams and their ages ranging from 2.5-3 years. The sample of the semen was diluted with Tris dilution with the addition of 10 ml of egg yolk per 100 ml, the sample by 1:1 with the tris and then the collection of sperm samples (Pooling) after the semen was divided into four treatments Control and bovine albumin at concentrations of 5, 7.5 and 10% and then complete dilution 10: 1, at 5C, dilution 20: 1, containing 5% glycerol per 100 ml. The results
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