This study was designed to investigate the effects of licorice extract (Glycyrrhiza glabra L.) addition to semen diluters on ram sperm progressive motility during storage at 5 ˚C for 72 h. Semen was collected from 3 proven Awassi rams. Licorice extract powder was added at levels of 1, 5, 10, 50 and 100 µg per ml. of diluter. Diluter containing no licorice extracts served as control (0).Progressive motility was estimated subjectively after dilution (0h), and at 24, 48, and 72 h of storage. The experiment was replicated 2 times with egg yolk-tris (EYT) diluter and 2 times with yolk- glucose-citrate diluter. Progressive motility increased significantly (p < 0.01) in levels of licorice extract 1, 5, 10, 50 and 100 µg / ml in both diluters, during all storage periods. The means of progressive motility were 72.5 ± 1.02 %, 72.08 ± 1.05, 70.90 ± 2.05 % and 66.25 ± 3.15 % respectively, compared to the control (0) 61.45 ± 16.2 %. Levels 1, 5 and 10 µg /ml were superior (p < 0.01) to levels 50 and 100 µg /ml. In conclusion, the addition of licorice extract to the diluter improved ram sperm progressive motility during cooled storage at 5 ˚C.
Background: The need for assisted reproduction technologies (ART) for the establishment of pregnancies has steadily increased worldwide. Therefore, it is of vital importance that an efficient sperm preparation technique used for retrieval of high-quality spermatozoa contributes to the creations of high-quality embryos, with high implantation potential.
Objective: to study the effect of swim up technique on human sperm motility and DNA integrity.
Subject and methods: A prospective study carried on 70 samples of human semen; each sample, divided into 2 parts, one part was prepared by swim-up technique and the other not, and then study sperm motility and DNA integrity in both parts. Sperm DNA integrity was determined using a modified
Non-thermal plasma(Dielectric barrier discharge) has many uses including living tissue sterilization, inactivation of the bacteria, excimer formation, angiogenesis, and surface treatment. This research aim is to use cold plasma as a tool to search the effect of the dielectric barrier discharge system at room temperature on human sperm motility and DNA integrity. This work was performed on 60 human semen samples suffering from low motility; each sample was prepared by centrifugation method, then each semen sample was divided into two sections, the first section is before significant exposure to the plasma system (DBD) and the second section is after treatment with the DBD system at ambient temperature. Before and after exposure to non-the
... Show MoreBackground: Sperm motility disorder is an important cause of infertility in male, and one of the causes of reduced motility of the sperm is the disorders of the mitochondria because it provides the required energy for sperm motility, Laser biostimulation or low-level laser therapy has a positive effect on the mitochondria and led to increasing the synthesis of ATP. Method: Twenty fresh human semen samples were used in this research study, each sample was separated into two portions, one was used as control which is not exposed to the laser beam and the other was irradiated with the wavelength of 410 nm diode laser with an output power of 100 mW and an exposure time of 60 seconds, then the measurement of
... Show MoreThe aim of this research to examine the impact of different level of Insulin certain biomarkers of local roosters semen after being cooling for varying lengths of periods, roosters semen pooled, diluted with extender, and distributed randomly among five groups: Diluted semen put through the semen cryopreservation technique; the control treatment (C1) 0 insulin; (C2) contained 4 IU/insulin; (C3) contained 5 IU/insulin; (C4) contained 6 IU/insulin; and (C5) contained 7 IU/insulin. After 0, 24, 48 and 72 hours, cooling semen were evaluated for plasma membrane integrity(MI), acrosome integrity(AI), malondialdehyde (MDA) and total antioxidant capacity (TCA). The result showed significant d
the energy that a radiation source emits as light (photons), electromagnetic waves, or particles is known as radiation. X-ray is one type of ionizing radiation that falls within the electromagnetic wave category of short wavelengths. Because of their short wavelength. X-ray is a type of ionizing radiation that is used in a constantly growing number of diagnostic procedures. X-ray diagnostic imaging has been employed in the medical field for more than a century(Hariyoto et al., 2020). Radiation has advantages for human health, but there is also a risk. the impacts of Radiation on Fertility it is an oligospermia, or a reduction in the number of sperm cells, which is already possible after radiation exposure of 0.15 mGy. It Leads to impede
... Show MoreThe possible effects of COVID-19 vaccines on reproductive health and male fertility in particular have been discussed intensely by the scientific community and the public since their introduction during the pandemic. On news outlets and social media platforms, many claims have been raised regarding the deleterious effects of COVID-19 vaccines on sperm quality without scientific evidence. In response to this emerging conflict, we designed this study to evaluate and assess the effect of the Pfizer-BioNTech mRNA COVID-19 vaccine on male fertility represented by the semen analysis parameters.
Background:
Germination and field emergence are delayed and their duration is prolonged due to the declining soil temperature during the spring season, which is reflected in the subsequent stages of crop growth, therefore, this study aimed to improve germination. Under a wide range of environmental conditions, a laboratory factorial experiment was carried out to study the effect of seed stimulation with potassium nitrate (distilled water only (0), 2, 4, and 6 mg L-1) and with an aqueous extract of licorice roots (distilled water only (0), 3, 6, and 9 g L-1) on the seed viability and vigor. The laboratory experiment was carried out according to the Completely Randomized Design (CRD) with four repetitions. The results showed the superiority of the intera
... Show MoreThe objective of this study was to investigate and find the relationships between human semen osmolality and oxidative stress with semen quality. Semen analysis was determined to evaluate semen quality and fertility. The concentration of semen malondialdehyde (MDA) was measured to assess oxidative stress. For this purpose, one hundred seventy healthy adult males were used in this study. The study was conducted from September 2018 to November 2019 in the Infertility care and in vitro fertilization center (IVF) and the Biology department of the College of Science at Salahaddin University in Erbil city. A significant positive correlation was observed between the total sperm motility, grade activity, and sperm motility index with semen osmol
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