Summary:
Background: the immune system differentiates between the endogenous and exogenous bodies that inter the body. The break-down of blood-testis barrier results in the production of antisperm antibodies. This may occur in the case of an infection to the prostate, seminal vesicle and epididymes. Antisperm antibodies (ASA) cause sperm agglutination and affect sperm motility, viability and sperm migration in the female reproductive tract. ASA also impair fertilization process. The objective of the present work was to study the effect of prednisolone on sperm motility index (SMI), viability and sperm penetration assay (SPA) in immunologically infertile men.
Materials and methods: The semen and serum samples of 140 infertile men were examined by microagglutination test and slide agglutination test to detect ASA and sperm agglutination. Semen fliud analysis was performed to report sperm motility index (SMI), sperm viability and hypo-osmotic swelling test (HOST). Sperm penetration assay was done to record sperm penetration rate (SPR), sperm decondensation rate (SDR) and sperm penetration index (SPI). Men with positive ASA were treated with prednisolone and considered as treated group Prednisolone was given orally in a dose of 5 mg three times daily for three months. The semen analysis, SMI HOST and SPA were performed before and after treatment with prednisolone. The number of semen samples in the treated group was 144 and in the control fertile group was 80. HOST-SPA positive semen was exposed to antisperm antibodies separation (ASAS) and invitro sperm activation prior to intra-uterine insemination.
Results: The SMI was significantly higher in the post-treated group compared to pretreated group (240 vs. 52.5, P<0.0l). The SPI in the control group was significantly higher than the post and pre-treated groups. The HOST and viability test results were significantly increased in the post-treated group compared to pre-treated group (73.42 vs. 48.56 and 71.36 vs, 50. 74 respectively, P<0.01). The sperm penetration rate, sperm decondensation rate and sperm penetration index were significantly increased in the post-treated vs. pre-treated groups (26.49 vs. 10.84, 10.91 vs. 3.47, P<0.05, 14.45 vs. 4.30, P<0.01 respectively). HOST-SPA positive semen was used for intra-uterine insemination (IUI). The semen was exposed to ASAS and in vitro activation prior to IUI and resulted in 42.86% pregnancy rate per cycle. The pregnancy was confirmed by the observation of gestation sac and viable fetal hear beet 5 weeks following IUI.
Conclusion: It was concluded from the results of the present study that treatment of immunological infertile men with prednisolone improves SMI, sperm viability and SPA results. The application of HOST-SPA positive semen for IUI resulted in successful pregnancy. The authors indicate that these viable spermatozoa with high fertilizable potential could be used for IVF and/or ICSI in immunologically infertile men.
This study was conducted to evaluate the prevalence rate of
toxoplasmosis among 294 rheumatoid arthritis (RA) patients treated with
methotrexate (MTX), 50 RA patients without treatment and 50 samples as
healthy control. Blood samples were collected and the presence of T.gondii
IgG and IgM antibodies was determined by using Enzyme linked
immunosorbent assay (ELISA). Tumor necrosis factor alpha (TNF-α) was
also estimated in serum of all subjects by using ELISA method too. The
seroprevalence of toxoplasmosis IgM and IgG in RA+MTX was
60(20.408%), and 98(33.33%), in RA patients 4(8%), and 18(36%) while,
it was 2(24%), 6(12%) in healthy group. Tumor necrosis factor alpha
(TNF-α) was also estimated in serum of a
Background: Profound alterations of respiratory function physiology accompany pregnancy; these conditions contribute to many of the disorders of the lung during pregnancy. The adaptive changes during the gravid period are designed to support maternal and fetal well-being during the special stresses of fetal growth and parturition Peak.
Objective: This study aimed to know the effect of pregnancy on peak expiratory flow rate in comparison with non-pregnant Iraqi women.
Method: This study was conducted on 255 healthy female at their reproductive ages , made up of 60 pregnant female in 1st trimester , 65 in 2nd trimester , 60 in 3rd trimester and 70 non pregnant as control group.
Results: There were a significant negative relation b
After about twelve months or maybe more, some people can’t achieve pregnancy. This might be a sign of infertility as a reproductive system disease. The following study was carried out to investigate the DAZ 1 gene methylation level and its association with azoospermia in Iraqi patients. One hundred and fifty human blood samples were collected from from different regions in Baghdad governorate, including (private medicals Labs and the high institute for infertility diagnosis assisted reproductive techniques and Kamal Al- Samara'ay IVF Hospital) from both fertile and infertile men. The control group consists of 50 samples ranging from 22 to 51 years old, while the patient (infertile group) consists of 100 samples ranging between 25 and 51 y
... Show MoreThe effects of scattering and secondary radiation generated inside the material on dose equivalent rate where studied using Co60 and Cs137 sources of activity (199.8 , 177.6) MBq , respectively for different thicknesses of Al , Pb and Pb- glass . The results showed that the equivalent rate increases when the effect of scattering was included for Al and Pb shields with cobalt-60 source of energy 1.25 MeV ; and decreases for Pb shield with Cs-137 source of energy 0.662MeV .The results showed also that the atomic number of The material effects the dose equivalent rate . The Pb-glass shield was found to be more efficient in absorption than other shields.
Background: Cerebral ischemia associated with Antiphospholipid Syndrome(APS) is a common arterial manifestation in adults.
Objectives: To look for the frequency of Antiphospholipid Antibodies (aPL), and their relation with other risk factors in young patients with cerebral ischemia.
Patients and methods: A retrospective study of 40 young patient’s ≤ 50 years with stroke collected from medical and neurological wards of Baghdad Teaching Hospital (BTH) from January - December 2009. All were inquired about the presence of risk factors of stroke, and sent for aPL including Anticardiolipin (ACL) and Lupus Anticoagulant (LA).
Results: Eight (20%) had ACL. Nine (22.5%) had LA. Both were present in 6(15%). ACL and/or LA were present
Background:
In this research the effect of cooling rate and mold type on mechanical properties of the eutectic
and hypoeutectic (Al-Si) alloys has been studied. The alloys used in this research work were (Al- 12.6%Si
alloy) and (Al- 7%Si alloy).The two alloys have been melted and poured in two types of molds with
different cooling rates. One of them was a sand mold and the other was metal mold. Mechanical tests
(hardness, tensile test and impact test) were carried out on the specimens. Also the metallographic
examination was performed.
It has been found that the values of hardness for the alloys(Al-12.6%Si and Al-7%Si) which poured in
metal mold is greater than the values of hardness for the same alloy when it poured in a heated
This study included the isolation of the parasite Entamoeba histolytica from bloody mucoid diarrhea specimens of patients suffering from amoebic dysentery and cultivation of parasite in Locke-egg medium (LEM) to study the effect of camel's milk and urine on the viability of E . histolytica with different concentrations (1 , 5 and 10 % ). The ability of camel's milk and urine in inhibiting the E . histolytica viability is examined after 24 and 48 h of incubation The results showed that the activity of camel's milk and urine was directly proportional with the increase in concentration and incubation period. Since the highest rate of parasite death reached ( 99.5% and 99.4%
... Show MoreThe current study was designed to evaluate the anti-inflammatory effect of GKB in the rat model of granulomatous inflammation. Thirty rats were distributed into five groups: The first group served as negative control group that received distilled water (DW) only without inducting inflammation, positive control group; treated with DW with the induction of inflammation and they were assigned to cotton pellet-induced granuloma, ginkgo biloba (GKB) treated group (200mg/kg/day), dexamethasone-treated group (1mg/kg), and Prednisolone treated group (5mg/kg). All the treatments were given orally for seven consecutive days. On day eight, the rats were anesthetized and the pellets together with granulation tissue were carefully removed
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