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The Relationship between Phylogenic Typing and Antimicrobial Susceptibility Patterns forEscherichia coliIsolatedfrom UTIs atMany Hospitals in Baghdad City
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Objective:The current study aime to isolate Escherichia colifrom urinary tract infections(UTIs) in many Baghdad hospitals. The study concentrate on phylogenic groups and this was done based on triplex PCRmethod by primers besieged to three genetic markers, chuA, yjaA and TspE4.C2. Evaluate the relationship of phylogenic groups of E. coli isolates with the antibiotic-non sensitive patterns. Methodology:Four hundredof E.coli bacteria isolated from urine samples from five hospitals in Baghdad city include: Ghazi AL-Hariri, Ibin- Al-Beledi , AL-Iskan , AL-Nooman and AL-Yarmoke hospitals. Phylogenetic categorization of E. coli isolates was completed by by means ofearlier reported triplex PCR-based on phylotypingprocedureusing primers besieged at three markers, chuA, yjaAand TspE4.C2. Phylogenetic combination was done on the starting point of the existence or nonexistence of the3 DNA fragments as follows: chuA–, TspE4.C2–, group A; chuA–, yjaA–, TspE4.C2+, group B1; chuA+, yjaA+, group B2; chuA+, yjaA–, group D. Because two possible profiles can be obtained for the groups A, B2, and D. Fifteen antibiotics second-hand for all foremost groups and their individual generations were used in this paper adjacent to all bacterial isolates. Results: The isolates ofE.colifrom UTI were distributed within thephylogroups B2 (43%);phylogroups D (28.25%);phylogroups A (24%) and B1 (3.5%), so phylogenic group B1 was prevalent among male patients of AL-Yermouk hospital ( 14%), followed AL-Iskan (3.75)% so, isolation rate of E.coli were higher among age (11-20 ) years (31.75 %), also(11%) were sensitivity to antibiotics, whilst (75.25%) were classified as extensive drug resistance pathogens, but (13.75% )were multidrug resistance and in Karkh areas, the frequency of phylogeneticB2 was (59.88%), followed phylogenetic A and D were ( 68.75 and 48.78%) respectively as compared to Rusafa areas were prevalence phylogenetic B2(40.12%),followedphylogenetic D and A were (51.22 and 31.25 %) , as well as most of these phylogenetic groups occurred more frequently in both groups B2 or D (43, 28.25%) respectively, also ( 2.75, 2.5% ) of phylogenic A were antibiotics sensitivity in both AL-Iskan hospital and Ghazi AL-Hariri hospital respectively whilst these phylogenic was multidrug resistance as percentage (9, 3.75) % respectively in both AL-Yermouk hospital and AL-Iskan hospital, Whilst in these hospital this phylogenic A was founded with extensive drug resistance (1, 0.75%) respectively, also most of phylogenetic group B2 occurred more frequently in AL-Yermouk hospital, so the sensitivity; multidrug resistance and extensive drug resistance as (2.25, 15 and 3.5%) respectively, also all phylogenetic groups E. coli isolates from UTI patients of five hospital weregrouped in two or three major group as A , B group contain major sub groups . Recommendations: The study recommends additional studies of the correlation between the Phylogenic group with antibiotic-resistance pattern for other bacterial types isolated from UTIs or the same bacteria isolated from other types of infections. From this study,it is recommendedto study onKlebsillaspp isolated from the infected patients urinewho submitted to the same hospitals orin the other provinces of the country.

 
 

 

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Publication Date
Fri Jan 01 2021
Journal Name
Materials Today: Proceedings
The optimal utilization of lighting factor in the flat photobioreactor for biological applications
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Distribution of light intensity in the flat photobioreactor for microalgae cultivation as a step design for production of bio-renewable energy was addressed in the current study. Five sizes of bioreactors with specific distances from the main light source were adopted as independent variables in experiential design model. The results showed that the bioreactor’s location according to the light source, determines the nature of light intensity distribution in the reactor body. However, the cross-section area plays an important role in determining the suitable location of reactor to achieve required light homogeneity. This area could change even the expected response of the light passing through the reactor if Beer-Lambert's law is adopted.

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Publication Date
Sat May 01 2021
Journal Name
Journal Of Physics: Conference Series
Anatomical histological structure of the cerebellum in the Iraqi frog Rana ridibunda ridibunda
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Abstract<p>The current study was designed to investigate the histological structure of the cerebellum in the Iraqi frog <italic>Rana ridibunda ridibunda</italic>. The cerebellum was removed surgically from 20 Iraqi frogs, <italic>Rana ridibunda ridibunda</italic>, and preserved in fixative of 10% formalin solution for a period of 72 hr, then the histological sections were prepared by the method of paraffin. The results of the study showed that the cerebellum of the adult Iraqi frog is one of the rhombencephalon regions, and consists of three layers, molecular layer, purkinje cells layer, and granular layer. The present histological findings suggest that the cerebellum of the </p> ... Show More
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Publication Date
Sat Jun 26 2021
Journal Name
Asian Journal Of Civil Engineering
Using AHP to prioritize the corruption risk practices in the Iraqi construction sector
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Publication Date
Tue Feb 13 2024
Journal Name
The International Journal Of Oral &amp; Maxillofacial Implants
The Accuracy of Intraoral Registration for Dynamic Surgical Navigation in the Edentulous Maxilla
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Purpose: Despite the high clinical accuracy of dynamic navigation, inherent sources of error exist. The purpose of this study was to improve the accuracy of dynamic navigated surgical procedures in the edentulous maxilla by identifying the optimal configuration of intra-oral points that results in the lowest possible registration error for direct clinical implementation. Materials and methods: Six different 4-area configurations were tested by 3 operators against positive and negative controls (8-areas and 3-areas, respectively) using a skull model. The two dynamic navigation systems (X-Guide® and NaviDent®) and the two registration methods (bone surface tracing and fiducial markers) produced four registration groups. The accuracy of the

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Publication Date
Tue May 01 2018
Journal Name
Journal Of Physics: Conference Series
The Effects of micro Aluminum fillers In Epoxy resin on the thermal conductivity
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Publication Date
Mon Mar 30 2020
Journal Name
Plant Archives
Survey of the insect pests from some orchards in the middle of Iraq
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Publication Date
Wed Jun 30 2021
Journal Name
Gsc Biological And Pharmaceutical Sciences
Checklist of the Umbelliferae family in the herbarium of Iraq natural history museum
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The aim of this study is to make the inventory for Umbelliferae family for the purpose of identifying the samples that were collected over long periods and saved in the herbarium of Iraq Natural History Museum/ University of Baghdad. We found that the herbarium plants were very large and varied from different parts for Iraq, in different and varied environments, which collected and arranged according to a very specific system in the herbarium for remaining an important source to all graduate students and researchers to take advantage for these plants. Also, the flowering and fruiting periods for these plants in Iraq were recorded in different regions. Most these plants begin to flower in the spring and thrive at fields and farms.

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Publication Date
Mon Nov 03 2025
Journal Name
Journal Of Baghdad College Of Dentistry
An in vitro evaluation of fit of the crowns fabricated by zirconium oxide-based ceramic CAD/CAM systems, before and after porcelain firing cycles and after glaze cycles
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Background: The objective of this in vitro study was to evaluate the vertical marginal fit of crowns fabricated with ZrO2 CAD/CAM, before and after porcelain firing cycles and after glaze cycles. Materials and Methods: An acrylic resin model of a left maxillary first molar was prepared and duplicated to have Nickel-Chromium master die. Ten die stone dies were sent to the CAD/CAM (Amann Girrbach) for crowns fabrication. Marginal gaps along vertical planes were measured at four indentations at the (mid mesial, mid distal, mid buccal, mid palatal) before (Time 0) and after porcelain firing cycles (Time 1) and after glaze cycles (Time 2) using a light microscope at a magnification of ×100. One way ANOVA LSD tests were performed to determine wh

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Publication Date
Wed Jan 31 2024
Journal Name
Journal Of The Faculty Of Medicine Baghdad (jfacmed Baghdad)
The Role of Activin A levels, Body Mass Index and Beta-Human Chorionic Gonadotropin in Ectopic Pregnancies and Missed Abortions – A Study on a Group of Iraqi Women
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Publication Date
Fri Feb 18 2022
Journal Name
Journal Of Blood Medicine
Circulating miR-126-3p and miR-423-5p Expression in de novo Adult Acute Myeloid Leukemia: Correlations with Response to Induction Therapy and the 2-Year Overall Survival
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Abstract Background: Acute myeloid leukemia (AML) results from sequential genetic alterations in a normal hematopoietic stem cell or its progenitors giving rise to an autonomous clone that dominates the bone marrow leading to marrow failure. MicroRNAs are short non-coding nucleic acid sequences that regulate post-transcriptional gene expression by base-pairing with their target mRNAs. MiRNAs can be secreted into extracellular fluids and carried to target cells by vesicles or bound to proteins. Intracellular and circulating miRNAs are believed to be useful markers in the diagnosis, prognosis, and treatment of various cancers. Practically, circulating miRNAs are more stable at room temperatures and extreme conditions. Purpose: This study aim

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