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Enzymatic Assay of Immobilized β-D-Galactosidase Enzyme on Magnetite Nanoparticle
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     According to the high operational costs, low stability, and reusability of enzymes, immobilization by nanoparticle gathering has increased in recent years. Iron oxide nanoparticles (magnetite nanoparticles, Fe₃O₄) have been prepared by mixing one volume of iron dioxide ions and two volumes of iron trioxide ions with HCl via the precipitation of iron salts by NH₄OH. The features of magnetic nanoparticles have been studied by Atomic Force Microscopy (AFM), X-Ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy, and Scanning Electron Microscopy (SEM). The prepared Fe3O4 was used in the adsorption method to immobilize the galactosidase enzyme. The immobilized enzyme has been compared with the crude one by optimizing time, PH, temperature, substrate solution concentration, and enzyme solution concentration. As a result, the immobilized enzyme has exhibited higher activity over long storage times, and a wide PH and temperature range than crude enzyme. In addition, whatever the substrate and enzyme concentrations, the activity of the immobilized enzyme increased over the non-immobilized one. As a result, a lower Km value for immobilized D-galactosidase indicates a higher affinity for the substrate than crude D-galactosidase. 

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Publication Date
Wed Mar 30 2022
Journal Name
Iraqi Journal Of Science
Zariski Topology of Intuitionistic Fuzzy d-filter
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In this paper we discuss the Zariski topology of intuitionistic fuzzy d-filter in d-algebra, with some topological properties on the spectrum of intuitionistic fuzzy d-filter in d-algebra X which have algebraic features such as connectedness. We find that this topology is a strongly connected, and  T0 space. We also define the invariant map on intuitionistic fuzzy prime d-filter with a homomorphism map.

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Publication Date
Thu Oct 16 2014
Journal Name
Journal Of The College Of Basic Education
Viscometric and Thermodynamic studies of D-galactose and D-maltose in sodium sulfide solution (0.25M) at deferent temperatures
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Publication Date
Wed Apr 20 2022
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Cubic Bipolar Fuzzy Ideals with Thresholds (α, β), ( ω ,ϑ) of a Semigroup in KU-algebra
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In this paper, we introduce the concept of cubic bipolar-fuzzy ideals with thresholds (α,β),(ω,ϑ) of a semigroup in KU-algebra as a generalization of sets and in short (CBF). Firstly, a (CBF) sub-KU-semigroup with a threshold (α,β),(ω,ϑ) and some results in this notion are achieved. Also, (cubic bipolar fuzzy ideals and cubic bipolar fuzzy k-ideals) with thresholds (α,β),(ω ,ϑ) are defined and some properties of these ideals are given. Relations between a (CBF).sub algebra and-a (CBF) ideal are proved. A few characterizations of a (CBF) k-ideal with threshol

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Publication Date
Thu Jun 25 2020
Journal Name
International Journal Of Drug Delivery Technology
Development and Characterization of Controlled Release Tablets of Candesartan Cilexetil/ β‑Cyclodextrin Inclusion Complex
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Objective: Matrix tablet approach is one of the delivery systems intended for poorly water-soluble drugs, like candesartan cilexetil (CC). CC is a class II drug used for the treatment of hypertension. Methods: Matrix tablets from (F1x to F18z) were prepared in the presence of β‑cyclodextrin. Matrix tablet formulation ensures control release of the drug and higher dissolution by β‑cyclodextrin. Fourier transform infrared spectroscopy (FTIR), and differential scanning calorimetry (DSC) were used to study compatibility. Results: The angle of repose determination showed good flow for most of the formulas, besides having good compressibility. Weight variation test for all formulas showed accepted value. Drug content measurement sho

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Publication Date
Thu Jun 25 2020
Journal Name
International Journal Of Drug Delivery Technology
Development and Characterization of Controlled Release Tablets of Candesartan Cilexetil/ β‑Cyclodextrin Inclusion Complex
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Objective: Matrix tablet approach is one of the delivery systems intended for poorly water-soluble drugs, like candesartan cilexetil (CC). CC is a class II drug used for the treatment of hypertension. Methods: Matrix tablets from (F1x to F18z) were prepared in the presence of β‑cyclodextrin. Matrix tablet formulation ensures control release of the drug and higher dissolution by β‑cyclodextrin. Fourier transform infrared spectroscopy (FTIR), and differential scanning calorimetry (DSC) were used to study compatibility. Results: The angle of repose determination showed good flow for most of the formulas, besides having good compressibility. Weight variation test for all formulas showed accepted value. Drug content measurement sho

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Publication Date
Thu Oct 01 2015
Journal Name
International Journal Of Current Engineering And Technology
Synthesis of Gold Nanoparticle in the form of Paper Willow by Hot Chemical Reduction Method
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Abstract: Colloidal gold nanoparticles (ringworm Palm or in the form of paper willow) have been prepared from HAuCl4 containing aqueous solution by hot chemical reduction method. The colloidal gold nanoparticles were characterized by SEM, EDX, and UV-VIS absorption spectroscopy. It was found that the variation of reduction time from boiling point affects the size of the nanoparticles and also in chemical reduction approach the size of nanoparticles can be controlled by varying the amount of variation the volume of reductant material with respect to the volume of HAuCL4.

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Publication Date
Fri Jan 26 2024
Journal Name
Iraqi Journal Of Science
Expression Of Xylose Reductase Enzyme From Spathaspora passalidarum In Saccharomyces cerevisiae.
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Baker’s yeast (Saccharomyces cerevisiae) has been genetically engineered to
ferment the pentose sugar xylose present in lignocellulosic biomass. One of the
reactions controlling the rate of xylose utilization is catalyzed by xylose reductase
(XR).The current study describes xylose reductase from Spathasporapassalidarum
with NADH preference. According to JGI site the gene coding for this enzyme
contains 954 nucleotides and it consists of 317 amino acids. The restriction sites for
the enzymes SacII and NotI located on the 5P
´
Ptermini for both the forward and
reverse specific primers were designed using Lasergen 9.0 program. The genomic
DNA was isolated and purified from S .passalidarum. Polymerase chain r

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Publication Date
Wed Dec 30 2020
Journal Name
Iraqi Journal Of Pharmaceutical Sciences ( P-issn 1683 - 3597 E-issn 2521 - 3512)
Docking Study of Naringin Binding with COVID-19 Main Protease Enzyme
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The Coronavirus Disease (COVID-19) has recently emerged as a human pathogen caused by SARS-CoV-2 virus was first reported from Wuhan, China, on 31 December 2019. Upon study, it has been used molecular docking to binding affinity between COVID-19 protease enzyme and flavonoids with evaluations based on docking scores calculated by AutoDock Vina. Results showed that naringin suppressed COVID-19 protease, as it has the highest binding value than other flavonoids including quercetin, hesperetin, garcina and naringenin. An important finding in this study is that naringin with neighboring poly hydroxyl groups can serve as inhibitors of COVID-19 protease bind to the S pocket of protein, it is shown that residues His163, Glu166, Asn142, His41and

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Publication Date
Fri Sep 01 2023
Journal Name
Cureus
The Evaluation of Dihydropyrimidine Dehydrogenase Enzyme Level in the Serum of Colorectal Cancer Iraqi Males on Fluoropyrimidine-Based Chemotherapy (Capecitabine)
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Publication Date
Sun Jan 01 2023
Journal Name
Caspian Journal Of Environmental Sciences
Efficacy of nanoparticle zinc oxide in the resistance of fungus Rhizoctonia solani causing black scurf disease in local potatoes
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