One of these plants utilized in traditional medicine is Lactuca seriolla Linn., which belongs to the Asteraceae family. It goes by a variety of common names in the world, including prickly lettuce, wild lettuce, jagged lettuce, and Kahu and khas. The work aimed to isolate and characterize some bioactive constituent(s) from the aerial part of Lactuca serriola utilizing  Combiflash NEXTGEN and high-performance liquid chromatography (HPLC). Lactuca serriola (aerial part) was extracted with 80% ethanol, then fractionated with hexane. Then 250 mg of hexane extract was mixed with 4 g of silica gel and loaded in cartilage, then bounded to the gold column of combi flash using a solvent system comprised of ethyl acetat

This study focusing on river water quality as a source of raw water, efficiencies of water treatment plants, the goodness of transporting water pipes in the water networks and drinking water quality as consumed within certain city residential sectors, in addition to seasonal variation. The following water quality parameters were studied: Electrical Conductivity, Turbidity, Dissolved oxygen, Biochemical oxygen demand, the concentration of nutrients like nitrogen and phosphate, the residual chlorine in addition to total bacterial count and total colon bacteria of raw water, and treated water of four selected water treatment plants in Mosul city. These plants are : the right united scheme, the new left scheme ,the old left scheme and the Gh

Glucoamylase from black Aspergillus niger isolate was purified by ammonium sulfate precipitation and sephadex G 200 filtration. A trial for the purification of glucoamylase resulted in an enzyme with a specific activity of 6472 unit/mg protein with 10 times fold. The main goal of the present work was to test this enzyme in hydrolyzing the raw starch. Two substrates were used: corn starch and potato starch 2% (w/v). The effect of enzyme dosage and thermal processing of substrate on kinetics and efficiency of hydrolysis were studied. The results suggested that the glucoamylase activity is increased as the increase of enzyme concentration and the enzyme was sufficiently effective in hydrolyzing tested raw starch, and thermal modification of

     This study included partial purification of the lipase enzyme from the karnel of the pecan nut. After applying ion exchange chromatography technique using CM-Cellulose, two lipase isoenzymes were observed with specific activity values of 2.66 and 1.7 units/mg protein. The highest activity of both isoenzymes appeared at the optimum pH values of 8 and 6 and at temperatures of 40 and 50 °C, respectively. A pure single band was obtained by using electrophoresis technique and it was found that the approximate molecular weight was 42 kDa for the two isoenzymes. The study dealt with the preparation of the diester compound 1,4-diacetoxybenzene, which was diagnosed by GC-MS and ¹H NMR spectrometry. When studying the inhibitio

This study was conducted to evaluate the efficacy of different techniques for extraction and purification of Tomato yellow leaf curl virus (TYLCV). An isolate of the virus free of possible contamination with other viruses infecting the same host and transmitted by the same vector Bemisia tabaci Genn. was obtained. This was realized by indicator plants and incubation period in the vector. Results obtained revealed that the virus infect Nicotiana glutinosa without visible symptoms, while Nicotiana tabaccum var. White Burley was not susceptible to the virus. The incubation period of the virus in the vector was found to be 21 hrs. These results indicate that the virus is TYLCV. Results showed that Butanol was more effective in clarification the

      This research describes a straightforward procedure for extracting the pigment of Methylene Blue (MB) dye from aqueous solutions by utilizing a low-cost, safe, natural, and national source. Batch adsorption experiments were carried out to determine contact time, adsorbent dose, and the starting concentration of the adsorbate. For the analysis, a UV spectrophotometer was employed. Dye adsorption equilibrium was obtained after 120 minutes of contact time. Temkin, Langmuir, and Freundlich isotherm adsorption were used at solution concentrations of (3, 4, 6, and 8) mg/l. Adsorption data is used to predict the pseudo first and pseudo second order kinetic equations, Elovich kinetic models, and intra-particle diffusion using pseudo f

Background: Recently epigenetic alterations have received increased attention because of theirimportant role in the process of tumerigenesis. It has been found that more than half of genetic changes were epigenetic. Epigenetic alterations are catalyzed by DNMTs enzymes. Increased knowledge about this molecular event may achieve progress in the war against cancer. The aim of this study was to evaluate and compare the expression of DNMT3B among oral, laryngeal and skin SCC. Materials and Methods: This study was performed on (120) formalin-fixed, paraffin-embedded blocks, histopathologically diagnosed as oral, laryngeal and skin SCC). Immunohistochemical staining of DNMT3B antibody was performed on each case of this study. Results: The immunoh

Abstract:

       The aim of the current study was to investigate the possible protective effect of graded doses (5, 10, and 15mg/kg) of pyridoxine hydrochloride intraperitoneally injected against (15mg/kg) doxorubicin-induced cardiotoxicity in female rats. Fifty-six (56) Wistar albino female rats were utilized weighing 180-200 gm allocated into eight groups, seven rats each; Group I: negative control distilled water; Group II: Pyridoxine (5mg/kg); Group III: Pyridoxine (10mg/kg); Group IV: Pyridoxine (15mg/kg); Group V: doxorubicin (15 mg/kg); Group VI: Pyridoxine (5 mg/kg) prior to

This study included the collecting of 390 gland toxicity of the Red Wasp Vespa orientalis and 418 gland toxicity of yellow wasp Polistes olivaceaus from different areas of Baghdad city. It was classified depending on taxonomic keys in order to diagnose and identify the general characteristics of each species, and then extract gland toxicity to get poison. The poison was purified by gel filtration throw Sephacryl S-200 obtain three peaks of proteins. The activity of lysis and clotting was estimated for peaks separate P1, P2 and P3 for wasp red toxin, the lysis activity of separate P1 was 98% of the poison origin activity and the clotting time was 47.3 minutes, while when estimating the activity of lysis and clotting for peaks separate P1,