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Molecular Detection of Enterotoxin Genes of Multiresistant Staphylococcus aureus Isolates from Different Sources of Food

Foodborne diseases are a major risk for human health. Millions of people become sick as a result of eating contaminated food with microorganisms that cause diseases. S.  aureus is considered as one of the most important pathogenic bacteria, having the ability to  activate certain genes that encode for heat stable enterotoxins and cause Staphylococcal food poisoning. Thus, this study aimed to determine the prevalence of multi resistant Staphylococcus aureus that produce enterotoxins in different sources of food . Forty nine isolates were identified as S.aureus, according to morphological and biochemical tests. They were isolated from 387 different food samples from several randomly covered restaurants and supermarkets in different regions of Baghdad. Molecular diagnosis of S. aureus using specific primers for the 16S rRNA gene was carried out by Polymerase Chain Reaction (PCR ) technique . Susceptibility of 43 isolates of S.aureus was tested against 15 antimicrobial agents. The results revealed that all the isolates were resistant (100%) to mecillinam, highly resistant to vancomycine and meropenemin  (74.4 %) and  moderately resistant to Oxacillin, Erythromycin Cefotaxime, and Cefiximein (67.4, 60.4, 62.8, , 60.5 %, respectively), while they showed low resistance to Gentamicin (34.8%). In addition, all of these isolates were susceptible  to Tigecycline and Amoxicillin/ clavulanic acid and Cefoxitin-Cloxacilin. High percentages of oxicillin resistant S. aureus were isolated from cooked food samples, followed by meat products, and with less percentage from pastry products. Molecular detection of enterotoxins A and B of Staphylococcus aureus isolates was performed using specific primers based on PCR. The results revealed that S. aureus isolated from cooked food had the highest percentage of the isolates producing the enterotoxins A and B. Type A enterotoxin gene showed a higher prevalence than type B gene among cooked food , dairy products and pastry. In conclusion, the results revealed a high prevalence of some classical enterotoxin genes in  multi-drug resistant S.aureus isolated from different sources of food, which can cause food-poisoning  and, consequently, a potential serious problem for public health.

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Publication Date
Fri Jun 30 2023
Journal Name
Iraqi Journal Of Science
Using Retrieved Sources for Semantic and Lexical Plagiarism Detection

     Plagiarism is described as using someone else's ideas or work without their permission. Using lexical and semantic text similarity notions, this paper presents a plagiarism detection system for examining suspicious texts against available sources on the Web. The user can upload suspicious files in pdf or docx formats. The system will search three popular search engines for the source text (Google, Bing, and Yahoo) and try to identify the top five results for each search engine on the first retrieved page. The corpus is made up of the downloaded files and scraped web page text of the search engines' results. The corpus text and suspicious documents will then be encoded as vectors. For lexical plagiarism detection, the system will

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Publication Date
Sun Oct 22 2023
Journal Name
Iraqi Journal Of Science
*E mail:[email protected] 1802 The Role of Antibiotic-Killed Staphylococcus aureus and Its DNA to Cause Arthritis in Rats

The purpose of this study is to investigate the capability of bacterial DNA compared to bacterial lysate in stimulating arthritis using rat model. One hundred mid-stream urine specimens were collected during November 2012 to January 2013, from patients suffering from urinary tract infections attending hospitals in Baghdad, Iraq. Susceptibility of Staphylococcus aureus isolates to antibiotics was examined. Twenty five isolates were identified as S. aureus and they developed multi drug resistance. S. aureus S1 lyaste and its DNA were intra-articulary injected in rats. The levels of IL-6, anti-ds DNA Ab and leukocytes count were measured. In general, IL-6, anti-ds DNA Ab and leukocytes count were significantly higher in sera of rats injecte

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Publication Date
Wed Dec 01 2021
Journal Name
Journal Of The Pakistan Medical Association 71(suppl 8)(12):
Ellagic acid-rich Pomegranate extracts synergizes moxifloxacin against methicillin resistance Staphylococcus aureus (MRSA)

To elucidate the anti- Methicillin resistance Staphylococcus aureus (MRSA) effect of pomegranate alone and in combination with moxifloxacin fluoroquinolone. A total of five clinical isolates of MRSA (ATCC 43300) were used in the study. Disc diffusion method was used to determine the anti-MRSA effect of pomegranate and/or moxifloxacin by using Mueller-Hinton agar. Minimal inhibitory concentration (MIC), fractional inhibitory concentration (FIC) of moxifloxacin and pomegranate were calculated, the dynamic picture of the bactericidal effect of pomegranate and/or moxifloxacin was determined. SPSS version 20.00 was used for data analysis. Zone of inhibition (ZOI) of moxifloxacin was 19.67±4.84mm which was not significant compared with pomegrana

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Publication Date
Wed Feb 08 2023
Journal Name
Iraqi Journal Of Science
Molecular Detection of Klebsiella pneumoniae serotype K2 Isolated Clinically

One hundred specimens (urine and sputum) were collected in sterilized containers from patients attending two hospitals in Baghdad province including: Educational Al-Karama Hospital and Educational Al-Yarmouk Hospital, in which, thirty-eight K. pneumoniae isolate were obtained out of one hundred specimens, all of which are of urine origin. The results obtained in this study showed that Klebsiella pneumoniae isolates were highly resistant to Augmentine (94.7%), also Klebsiella isolates were resistant to Ceftriaxone (86.8%), Cefotaxime (81.5%), and Ceftazidime (65.7%). Very low level of resistance was found toward Imipenem and Meropenem (18.4% and 5.2%) respectively. Only four isolates (10.52 %) were positive for wzy gene; K19, K20, K21, an

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Publication Date
Wed Jun 15 2022
Journal Name
Journal Of Baghdad College Of Dentistry
Molecular Detection of Porphyromonas gingivalis in COVID-19 Patients

Background:SARS-CoV-2 infection has caused a global pandemic that continues to negatively impact human health. A large group of microbial domains including bacteria co-evolved and interacted in complex molecular pathogenesis along with SARS-CoV-2. Evidence suggests that periodontal disease bacteria are involved in COVID-19, and are associated with chronic inflammatory systemic diseases. This study was performed to investigate the association between bacterial loads of Porphyromonas gingivalis and pathogenesis of SARS-CoV-2 infection. Fifty patients with confirmed COVID-19 by reverse transcriptase-polymerase chain reaction, their age ranges between 20-76 years, and 35 healthy volunteers (matched accordingly with age and sex to th

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Publication Date
Thu Feb 01 2024
Journal Name
Baghdad Science Journal
Molecular study of the relationship of gene expression of some genes with the temperature variation of bacterial growth

Pseudomonas aeruginosa is an opportunistic pathogen responsible for serious infections. At least three different exopolysaccharides, alginate, polysaccharide synthesis locus (Psl), and pellicle exopolysaccharide (Pel) make up the biofilm matrix in P. aeruginosa . The effect of temperature on the biofilm formation and gene expression was examined by microtiter plate and real-time quantitative polymerase chain reaction (qRT-PCR). To be able to determine the effect of temperature on biofilm formation and gene expression of P. aeruginosa, 303 clinical and environmental samples were collected. Pseudomonas aeruginosa was isolated from 61 (20.1%) and 48 (15.8%) of the clinical and e

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Publication Date
Wed Aug 09 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Effect of Ascorbic Acid on Pathogenic Staphylococcus aureus Growth singly and in Combination with Some Antibiotics

 One hundred and fifteen isolates of Staphylococcus aureus were isolated from human infected wound and burn. The resistance of this organism for eight commonly used antibiotics was determined by disc diffusion methods.          The minimum inhibitory concentration MIC was determined for each drug by tube dilution method. Results showed significant variations in the MIC values. It was found that two isolates had high resistance for the eight antibiotics used. Effect of ascorbic acid on bacterial growth was studied singly and in combination with antibiotics. Reductions in MIC value were reported for different antibiotics when tested by growth curve method too. 

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Publication Date
Wed Dec 01 2021
Journal Name
Iraqi Journal Of Science
Antibacterial activity of Rosmarinus officinalis and Dodonaea viscosa leaves extracts against Escherichia coli and Staphylococcus aureus

The isolates from urine and synovial fluid samples of Escherichia coli and Staphylococcus aureus bacteria were isolated and identified, in order to study alternative treatment or antibacterial agents of plant extracts from Rosmarinus officinalis and Dodonaea viscosa plants. The isolates were identified by using cultural and biochemical tests, in addition to API 20E kit as confirmation test. The results exhibited that Rosemary extract prevents the biofilm formation and causes inhibition to bacterial growth, while the doddonia extract causes antibacterial activity on the tested bacteria.

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Publication Date
Sun Dec 29 2019
Journal Name
Iraqi Journal Of Science
Phylogenetic tree analysis based on the 16S sequence alignment for Klebsiella spp. isolated from different sources

16S ribosomal RNA (16S rRNA) gene sequences used to study bacterial phylogeny and taxonomy have been by far the most common housekeeping genetic marker utilized for identification and ancestor determination. This study aimed to investigate, for the first time, the relationship between Klebsiella spp. isolated from clinical and environmental samples in Iraq.

Fifty Klebsiella spp. isolates were isolated from clinical and environmental sources. Twenty-five isolates were collected from a fresh vegetable (Apium graveolens) and 25 from clinical samples (sputum, wound swab, urine). Enteric bacteria were isolated on selective and differential media and identified by an automatic identification system, vitek-2.

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Publication Date
Sun Sep 29 2019
Journal Name
Iraqi Journal Of Science
Phylogenetic tree analysis based on the 16S sequence alignment for Klebsiella spp. isolated from different sources

16S ribosomal RNA (16S rRNA) gene sequences used to study bacterial phylogeny and taxonomy have been by far the most common housekeeping genetic marker utilized for identification and ancestor determination. This study aimed to investigate, for the first time, the relationship between Klebsiella spp. isolated from clinical and environmental samples in Iraq.

     Fifty Klebsiella spp. isolates were isolated from clinical and environmental sources. Twenty-five isolates were collected from a fresh vegetable (Apium graveolens) and 25 from clinical samples (sputum, wound swab, urine). Enteric bacteria were isolated on selective and differential media and identified by an automatic identif

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