The purpose of this study is to investigate the capability of bacterial DNA
compared to bacterial lysate in stimulating arthritis using rat model. One hundred
mid-stream urine specimens were collected during November 2012 to January 2013,
from patients suffering from urinary tract infections attending hospitals in Baghdad,
Iraq. Susceptibility of Staphylococcus aureus isolates to antibiotics was examined.
Twenty five isolates were identified as S. aureus and they developed multi drug
resistance. S. aureus S1 lyaste and its DNA were intra-articulary injected in rats. The
levels of IL-6, anti-ds DNA Ab and leukocytes count were measured. In general, IL-
6, anti-ds DNA Ab and leukocytes count were significantly highe

Background: Bacterial DNA released upon bacterial autolysis or killed by antibiotics, hence, many inflammatogenic reactions will be established leading to serious tissue damage. Aim: the present work aimed to elucidate the histopathological changes caused by prokaryotic (bacterial) DNA and eukaryotic (candidal) DNA. Materials and methods: twenty one Staphylococcus aureus and 36 Candida albicans isolates were isolated from UTI patients. Viable cells and DNA of the highest antibiotic sensitive isolates were injected, intraurethraly, in mice. Results were evaluated via histopathological examination. Results: Mildest reactions were obtained from mice challenged with viable C. albicans compared with those challenged with viable S. aureus. Dos

In This study a comparison between Proteus mirabilis DNA and Candida albicans DNA in Arthritis. Fourteen Proteus isolates (11.6%) were collected from 120 specimens collected from midstream urine of patients of both sex and different ages attending different hospitals in Baghdad. Antibiotic sensitivity assay showed that All Proteus mirabilis recovered from UTI developed multidrug resistance and variable degree of resistance. Histopathological changes in model treated with Proteus mirabilis DNA revealed congestion, inflammatory cells infiltration, oedema, hemorrhagic exudates as well as necrotic cells. Furthermore, articular joints damage has been noticed with articular tissue in ligament and lining epithelium. In addition, an aggregation

Escherichia coli  infections are becoming difficult treated because of extensive resistance to antibiotic among these organisms and manufacturing extended-spectrum beta lactamases enzymes (ESBLs) make them resistant to beta-lactam antibiotics. This study aims to offer a summary of the main horizontal transmission  apparatuses  between E. coli as well as  Staphylococcus aureus and emergence resistance to antibiotics. Fifty of the E. coli and  50 of S. aureus isolates were examined to obtain minimum inhibitory concentration (MIC) results. These isolates were then tested by  conventional polymerase chain-reaction for the existence or absenc

Background: Staphylococcus aureus infections are growing problems worldwide with important implications in hospitals. The organism is normally present in the nasal vestibule of about 35% apparently healthy individuals and its carriage varies between different ethnic and age groups.

Objective:To study the antibiotic resistance of staphylococcus aureus isolated from nasal cavity of Health Care Personnel.

Patients and methods: A total of 180 samples were collected from the nose of the  two groups (health care personnel, community control) at Baghdad Teaching Hospital. They were screened for nasal colonization with S.aureus during the period between Apr

Methicillin resistant Staphylococcus aureus (MRSA) is one of the principal nosocomial causative agents. This bacterium has the capability to resist wide range of antibiotics and it is responsible for many diseases like skin, nose and wounds infection. In this study, randomly amplified polymorphic DNA (RAPD)-PCR was applied with ten random primers to examine the molecular diversity among methicillin resistant Staphylococcus aureus (MRSA) isolates in the hospitals and to investigate the genetic distance between them. 90 Isolates were collected from clinical specimens from Iraqi hospitals for a total of 90 isolates. Only 10 strains (11.11%) were found to be MRSA. From these 10 primers, only 9 gave clear amplification products. 91 fragment l

Brucellosis is one of the five common bacterial zoonoses in the world caused by
organisms belonging to the genus Brucella. Immune recognition of bacterial
infection may contribute to cytokine, as well as antibody production that are
characteristic of innate and adaptive responses. In this study, the presence of
attenuated live Brucella melitenses Rev1 bacteria or its DNA induced the immune
system to produce IFN-γ and anti-ds DNA antibody. In respect to IFN-γ released,
the B. melitensis Rev 1 attenuated live vaccine was able to stimulate the immune
system more than the DNA (P≤0.05). Such finding could be attributed to the whole
attenuated bacteria that have immunogenic factors other than the DNA like cell wall<

  This study is a trail to know if the genes controlling some of heavy metals resistance ( lead, zinc, cadmium, cromium) in two types of pathogenic bacteria  E. coli  as gram negative bacteria and S. aureus as gram positive bacteria, present on the β-lactamase plasmid.     Ten isolates of each bacterial types which produced β-lactamase enzyme, were cultivated in the presence of acridine orange. The growing in the presence of acridine orange resulted in loss of the β-lactamase genes in S. aureus and E. coli, and loss of the heavy metals resistance in S. aureus, while the resistance of E. coli against heavy metals still without any change.   The results indicate that the genes for heavy

E. coli was isolated, and it was Gram-negative rod bacteria that was colony circular, regular edged, thick somewhat glitter and  viscous(less). It was lactose fermenter bacteria and belongs the family of Enterobacteriaceae. E. coli showed sensitivity to all used antibiotics except Erythromycin (E), Cloxacellin (CX), Rifampin (RA), Cephalothin (KF), Ampicillin (AM), and Penicillin (P). The experimental results of antibiotic sensitivity of E. coli in media containing different concentrations of omeprazole, a proton pump inhibitor, showed an enhancement of resistance by decreasing the sensitivity of E. coli inversely with drug concentration against the antibiotics that E. coli was sensitive to. It seems that omeprazole changed cell mem