Methicillin resistant Staphylococcus aureus (MRSA) is one of the principal nosocomial causative agents. This bacterium has the capability to resist wide range of antibiotics and it is responsible for many diseases like skin, nose and wounds infection. In this study, randomly amplified polymorphic DNA (RAPD)-PCR was applied with ten random primers to examine the molecular diversity among methicillin resistant Staphylococcus aureus (MRSA) isolates in the hospitals and to investigate the genetic distance between them. 90 Isolates were collected from clinical specimens from Iraqi hospitals for a total of 90 isolates. Only 10 strains (11.11%) were found to be MRSA. From these 10 primers, only 9 gave clear amplification products. 91 fragment l

Dermatophytes are a group of morphologically and physiologically related molds some of which cause well defined infections: dermatophytoses (tineas or ringworm). The present study aims at identification of dermatophytes species and varieties from patients in Wasit province-Iraq using molecular approach based PCR fingerprint.
The short oligonucleotide (GACA)4 is a microsatellite primer was used in this study for identification of dermatophyte isolates. The results identified different species and varieties among dermatophytes. The numbers of resulting PCR bands ranged from 1 to 4 (size range, 600bp to 1600bp) for each species. The resulting patterns were distinct for Trichophyton and Microsporum species and varieties.
Trichophyton s

      This study includedepidermal appendages characters of ten species belongingto the familyAsteraceae.Most of the studied species are characterized by eglandular, glandular, trichomesand papillae which are variable according to itskind, number of cells, shape, dimensions, characteristicsof surrounding cells, wall thickness and the position. Results showed that all species included glandular and eglandulartrichomes exceptCosmos bipinnatus, Ecliptaprostrate, andEupatoriumcannabinum that lacked in glandular trichomes and were based on their specific details.Trichomes were divided into different groups. The glandular ones involved sessile glandular or group multicellular and the head wasmace-shaped or round

       The aim of this study was to investigate the genetic diversity and markers associated with salinity tolerance in three genotypes of wheat created for salt tolerance by plant breeding program, as well as two Iraqi varieties using random amplified polymorphic cDNA (RAPD-PCR) with eight primers were used. The results of RAPD marker revealed that there are genetic variations in several particular segments of various sizes between the selected genotypes and the local varieties with more genetic variation except for (OPG-09) did not appear any band with the selected genotypes and local cultivars.  The results of the phylogenetic tree analysis (cluster) based on the presence or absence of DNA amplified for each primer were used to

This study aimed at isolating uropathogenic Escherichia coli from urinary tract infections (UTIs) of human and cattle to examine the molecular diversity and phylogenetic relationship of the isolates. A total of 100 urine samples were collected from UTIs of human and cattle. The isolates identification was done using routine diagnostic methods and confirmed by Vitek2. Antimicrobial susceptibility was tested against 10 antimicrobials. Random amplified polymorphic DNA (RAPD)-polymerase chain reaction (PCR) was applied to identify the genetic diversity among E. coli isolates from human and animal origin by using five different octamer primers. The gelJ software for the phylogenetic analysis created Dendrograms. Out of 50 human urine samples, E.

This study focuses on diagnosis of Candida species causing Vulvovaginal Candidiasis using phenotype and genotype analyzing methods, and frequencies of candida species also using Vulvovaginal Candidiasis patients. 130 samples (100 from patients and 30 from non infected women) were collected and cultured on biological media. Identifying the yeasts, initially some phenotypic experiments were carried out such as germ tube, from motion of pseudohyphae and clamydospores in CMA+TW80 medium, API20 candida and CHROMagar Candida. Genomic DNA of all species were extracted and analyzed with PCR and subsequent Polymerase Chain Reaction - Restriction Fragments Length Polymorphism (PCR-RFLP) methods. Frequency of C. albicans, C. krusei, C. tropicalis , C.

In this study, 25 clinical isolates of Proteus spp. were collected from urine, wounds and burns specimens from different hospitals in Baghdad city, all isolates were identified by using different bacteriological media, biochemical assays and Vitek-2 system. It was found that 15 (60%) isolates were identifies as Proteus mirabilis and 10 (40 %) isolates were Proteus vulgaris. The susceptibility of P. mirabilis and P. vulgaris isolates towards cefotaxime was (66.6 %) and (44.4 %) respectively; while the susceptibility of P. mirabilis and P. vulgaris isolates towards ceftazidime was (20%). Extended spectrum β-lactamses producing Proteus was (30.7 %). DNA of 10 isolates of P. mirabilis and 4 isolates of P. vulgaris were extracted and detecti