In accordance with epidemic COVID-19, the elevated infection rates, disinfectant overuse and antibiotic misuse what led to immune suppression in most of the population in addition to genotypic and phenotypic alterations in the microorganisms, so a great need to reevaluate the genetic determinants that responsible for bacterial community (biofilm) has been raised. A total of 250 clinical specimens were obtained from patients in Baghdad hospitals and streaked on Mannitol salt agar medium. The results revealed that 156 isolates appeared as round yellow colonies, indicating that they were mostly identified as Staphylococcus aureus from 250 specimens. The antibiotic resistance pattern of the isolates for methicillin 37.17% (n=58), Amoxicillin-Clavulanate 58.9% (n=92), chloramphenicol 6.4% (n=10), Tetracyclin 62.8% (n=98), ceftriaxone 53.8% (n=84), Ciprofloxacin 6.4% (n=10), Gentamicin 42.3% (n=66), levofloxacin 28.2% (n=44), Penicillin 33.3% (n=52). The results demonstrated that 49 isolates were multidurg resistance. The biofilm formation ability of MDR was detected and total of 120 S. aureus isolates (76.92 %) were found to be adherent to varied degrees. Only fifty isolates (32.05% of the total) were classified as strong biofilm producers. Twenty-three (14.75%) were moderate producers, and forty seven isolates (30.12%) were found to be weak producers.
The problem of the high peak to average ratio (PAPR) in OFDM signals is investigated with a brief presentation of the various methods used to reduce the PAPR with special attention to the clipping method. An alternative approach of clipping is presented, where the clipping is performed right after the IFFT stage unlike the conventional clipping that is performed in the power amplifier stage, which causes undesirable out of signal band spectral growth. In the proposed method, there is clipping of samples not clipping of wave, therefore, the spectral distortion is avoided. Coding is required to correct the errors introduced by the clipping and the overall system is tested for two types of modulations, the QPSK as a constant amplitude modul
... Show MoreIn this paper different channel coding and interleaving schemes in DS/CDMA system over multipath fading channel were used. Two types of serially concatenated coding were presented. The first one composed of Reed-Solomon as outer code, convolutional code as inner code and the interleaver between the outer and inner codes and the second consist of convolutional code as outer code, interleaved in the middle and differential code as an inner code. Bit error rate performance of different schemes in multipath fading channel was analyzed and compared. Rack receiver was used in DS/CDMA receiver to combine multipath components in order to enhance the signal to noise ratio at the receiver.
Seventy-six urine specimens were collected from of patients suffering from recurrent
urinary tract infections (UTIs). Specimens were bacteriologically analyzed, fifty
(65.8%) of isolated bacterial strains were belonged to E.coli. 100% of isolated
uropathogenic E.coli (UPEC)strains displayed a biofilm positive phenotype under
optimized condition using microtiter plate assay. 21 of E.coli strains classified as highly
positive biofilm producers (42%), and 29 (58%) as weakly positive biofilm producers.
The D-enantiomers of amino acids have been thought to have relatively insignificant function in biological processes like, D-amino acids are sometimes found in proteins that are not synthesized by ribosomes. While L-amino acids clearly permanent in nature, D-amino acids have previously inapprehensible regulatory roles in the bacterial kingdom, any diverse of bacterial phyla made from these D-amino acids regulate cell wall remodeling in stationary phase and cause biofilm dispersal in aging bacterial communities. Clarification the mechanism by which D-amino acids given cell wall reorganization and biofilm disassembly will undoubtedly discover new paradigms for understanding how extra cytoplasmic processes are regulated as well as lead to d
... Show MoreObjectives: This study aims to broaden our knowledge of the role of eDNA in bacterial biofilms and antibiotic-resistance gene transfer among isolates. Methods: Staphylococcus aureus, E. coli, and Pseudomonas aeruginosa were isolated from different non-repeated 170 specimens. The bacterial isolates were identified using morphological and molecular methods. Different concentrations of genomic DNA were tested for their potential role in biofilms formed by study isolates employing microtiter plate assay. Ciprofloxacin resistance was identified by detecting a mutation in gyrA and parC. Results: The biofilm intensity significantly decreased (P < 0.05) concerning S. aureus isolates and insignificantly (P > 0.05) concernin
... Show MoreThe pathogenicity of S. saprophyticus was studied in mice. A group of white mice were injected transurethrally using a catheter with S. saprophyticus S67 cell suspension in a concentration reached 109 CFU/ml. concomitantly, the role of its peptidoglycan in the pathogenicity was studied by injecting another group of mice with 0.3 mg/0.2 ml of partially purified S. saprophyticus S67 peptidoglycan extract. After autopsy, kidneys and urinary bladder showed several histopathological changes both in cells and peptidoglycan injected mice, included: hydropic degeneration, glomerulus shrinkage, congestion of renal vessels, infiltration of inflammatory cells, and dekeratinization in urinary bladder.
Many species are resistant to heavy metals in their surrounding polluted environment and Staphylococcus sp. is an example. This study aimed to isolate and characterize bacteria resistant to heavy metals in the Shatt Al-Arab River in southern Basra, Iraq. Based on the morphology and using Vitek II system, and due to their high resistance to heavy metals (mercury and chromium), two species of Staphylococcus (Staphylococcus lentus and Staphylococcus lugdunensis) were chosen and isolated. The minimum inhibitory concentration (MIC) of the isolates against Hg and Cr was determined after 72 h. of incubation in solid media. All isolates were resistant to Hg (2000 mgL-1) and Cr (4000mgL
... Show MoreIris research is focused on developing techniques for identifying and locating relevant biometric features, accurate segmentation and efficient computation while lending themselves to compression methods. Most iris segmentation methods are based on complex modelling of traits and characteristics which, in turn, reduce the effectiveness of the system being used as a real time system. This paper introduces a novel parameterized technique for iris segmentation. The method is based on a number of steps starting from converting grayscale eye image to a bit plane representation, selection of the most significant bit planes followed by a parameterization of the iris location resulting in an accurate segmentation of the iris from the origin
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