Gingival crevicular fluid (GCF) may reflect the events associated with orthodontic tooth movement. Attempts have been conducted to identify biomarkers reflecting optimum orthodontic force, unwanted sequallea (i.e. root resorption) and accelerated tooth movement. The aim of the present study is to find out a standardized GCF collection, storage and total protein extraction method from apparently healthy gingival sites with orthodontics that is compatible with further high-throughput proteomics. Eighteen patients who required extractions of both maxillary first premolars were recruited in this study. These teeth were randomly assigned to either heavy (225g) or light force (25g), and their site specific GCF was collected at baseline and after 1hr, 1day, 7days, 14days, 21days and 28days post force application. Periostrips were used for GCF collection and subsequent phosphate buffered saline (PBS) was used for immediate protein elution with centrifugal speed of 10000rpm for 5min and stored at -80°C. Protein concentration was estimated using Bradford colorimetric assay. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) was carried out to resolve the purity of proteins in the collected samples and the method of collection was validated by western immuno-blotting of alpha amylase salivary enzyme. The current collection, storage and protein extraction protocol showed the best protein recovery and purity with validated collection free of salivary contamination. In conclusion, tiny GCF volume from healthy sites and evaporation issues of such promising non-invasive fluid motivate us to investigate a standardized protocol enabling optimal preservation of GCF sample and the currently followed protocol may serve as a reference for future proteomic studies searching for GCF biomarkers in diagnosing and monitoring orthodontic tooth movement.
Abstract: The aim of the current research is to find out the extent to which systems thinking skills are included in the mathematics textbook scheduled for the third intermediate grade for the academic year (2020-2021) by answering the main research question: What are the systems thinking skills included in the mathematics textbook for middle third grade? The analytical descriptive approach was used, and to achieve the goal of the research, a list of the main systemic thinking skills and sub-skills was prepared, and after analyzing the content of the mathematics textbook, the reliability of the analysis was verified through the analysis over time and through others, and it obtained a reliability rate of 98% us
... Show MoreThe marshes are one of the important environmental features affecting human and animal systems, so the studying of changes they undergo is one of the important topics. This study is concerned with the changes occurring in the Al Saadya marsh for the period from 1987 to 2017 exclusively in the winter season (the marshes’ revival season in Iraq revive). In order to inspect the changes in this marsh, we choose 7 years to cover the study period as a criterion years, namely 1987, 1990, 1995, 2000, 2007, 2014 and 2017. The “Maximum Likelihood” classifier was used to separate the stacked land cover features, where the minimum overall accuracy ratio that recorded for all years of study was 96%. The results revealed that Al-Saadya marsh went t
... Show MoreRecently, the environmental isotopes are adopted to figure out the hydrological processes, recharge areas, flow paths, groundwater origin and the interaction between different watery bodies. Currently, five samples of the rainwater have been collected since January to April 2012, as well as December 2011. Those sampling periods have highest amounts of precipitation events. Meantime, 25 samples of groundwater, 5 of the Lesser Zab River and 3 of overland flow have been picked up during the wet period. The dry sampling of groundwater and the Lesser Zab River has been achieved in summer 2011. The Local Meteoric Water Line lies between Global Meteoric Water Line (GMWL) and East Mediterranean Water Line (EMWL). The lowest, highest and
... Show MoreAudio security is an important aspect in various areas of communication. This paper deals with audio encryption as many of the data communication depends on audio data. In this paper, a new proposal of audio encryption system has been introduced. The system can be divided into two phases, the first phase focuses on generating a high-quality Pseudo Random Number generator (PRNGs) using elementary, periodic and hybrid rules of cellular automata (CA). The system suggests a new combination of CA rules in an endeavor to provide high randomness and to improve the strength of the proposed cryptosystem. Whereas the second phase produces the Enhanced Rivest Cipher 5 (ERC5) algorithm which employs the generated Random Number Sequence (RNS) i
... Show MoreIraq is one of the Arabian area countries, which considered from the drier areas
on the earth, though it has two main rivers that pass through(Tigris and Euphrates);
it suffers the same problem as them (drought), only the rivers' nearby regions make
use of their water for (domestic, agricultural, and industrial purposes(.
One of the usable solutions is to utilize the groundwater (especially in the desert
regions). Using the Remote Sensing and geographic information system is a rapid
and coast effective techniques, they provide information of large and inaccessible
area within short span for assessing, monitoring, and management of groundwater
resources. In this study, an adaptive algorithm based on Canny edge dete
The COVID-19 pandemic has had a huge influence on human lives all around the world. The virus spread quickly and impacted millions of individuals, resulting in a large number of hospitalizations and fatalities. The pandemic has also impacted economics, education, and social connections, among other aspects of life. Coronavirus-generated Computed Tomography (CT) scans have Regions of Interest (ROIs). The use of a modified U-Net model structure to categorize the region of interest at the pixel level is a promising strategy that may increase the accuracy of detecting COVID-19-associated anomalies in CT images. The suggested method seeks to detect and isolate ROIs in CT scans that show the existence of ground-glass opacity, which is fre
... Show MoreMinimizing the power consumption of electronic systems is one of the most critical concerns in the design of integrated circuits for very large-scale integration (VLSI). Despite the reality that VLSI design is known for its compact size, low power, low price, excellent dependability, and high functionality, the design stage remains difficult to improve in terms of time and power. Several optimization algorithms have been designed to tackle the present issues in VLSI design. This study discusses a bi-objective optimization technique for circuit partitioning based on a genetic algorithm. The motivation for the proposed research is derived from the basic concept that, if some portions of a circuit's system are deactivated during th
... Show MoreThis study proposed control system that has been presented to control the electron lens resistance in order to obtain a stabilized electron lens power. This study will layout the fundamental challenges, hypothetical plan arrangements and development condition for the Integrable Optics Test Accelerator (IOTA) in progress at Fermilab. Thus, an effective automatic gain control (AGC) unit has been introduced which prevents fluctuations in the internal resistance of the electronic lens caused by environmental influences to affect the system's current and power values and keep them in stable amounts. Utilizing this unit has obtained level balanced out system un impacted with electronic lens surrounding natural varieties.
The virtual decomposition control (VDC) is an efficient tool suitable to deal with the full-dynamics-based control problem of complex robots. However, the regressor-based adaptive control used by VDC to control every subsystem and to estimate the unknown parameters demands specific knowledge about the system physics. Therefore, in this paper, we focus on reorganizing the equation of the VDC for a serial chain manipulator using the adaptive function approximation technique (FAT) without needing specific system physics. The dynamic matrices of the dynamic equation of every subsystem (e.g. link and joint) are approximated by orthogonal functions due to the minimum approximation errors produced. The contr
Innovative laboratory research and fluid breakthroughs have improved carbonate matrix stimulation technology in the recent decade. Since oil and gas wells are stimulated often to increase output and maximum recovery, this has resulted in matrix acidizing is a less costly alternative to hydraulic fracturing; therefore, it is widely employed because of its low cost and the fact that it may restore damaged wells to their previous productivity and give extra production capacity. Limestone acidizing in the Mishrif reservoir has never been investigated; hence research revealed fresh insights into this process. Many reports have stated that the Ahdeb oil field's Mishrif reservoir has been unable to be stimulated due to high injection pressures, wh
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