This study aimed to detect Anaplasma phagocytophilum in horses through hematological and molecular tests. The 16S rRNA gene of the Anaplasma phagocytophilum parasite was amplified by polymerase chain reaction (PCR), then sequenced, and subjected to phylogenetic analysis to explore "Equine Granulocytic Anaplasmosis" (EGA) infection in three important gathering race horses areas in Baghdad governorate, Iraq. Blood samples were obtained from 160 horses of varying ages, three breeds, and both sexes, between January and December 2021. Prevalence and risk variables for anaplasmosis were analyzed using statistical odds ratio and chi-square tests. Results demonstrated that clinical anaplasmosis symptoms comprised jaundice, weight loss, paleness of mucus membrane with petechial hemorrhage in the third elides, and edema in extremities; There was no tick infestation. The hematological test did not significantly reveal decreases in red and white blood cells and platelet count. Microscopically found 11 from 160 smears (6.88%) had morulae within granulocytes, PCR results of Anaplasma spp primers was 32 positive amplicons (20%), and molecular sequencing results of “16S ribosomal RNA genes” confirmed 21 horses (13.13%) infected by Anaplasma phagocytophilum for the first time in Iraq horses. The results of the phylogenetic analysis revealed compatibility values similarity 98.81-99.76% with worldwide isolates. Mares occurred not significantly riskier; also age and breed were not illustrated risks of any group. This study is the first molecular detection of Anaplasma phagocytophilum in racehorses reared in Baghdad in Iraq. The outcomes of this study provide genetic data for early identification of Anaplasma phagocytophilum infection, treatment, and management of the illness in Iraq horses, as well as monitoring its transmission to the human population.
During the period from September 2013 till the end of July 2014 ,a total of 340 birds Passer domesticus were collected from Tikrit city . The study revealed the infection of birds with seven species of cestoda helminthes , belonging to the genus Raillietin . These species included R. tetragona , R. echinobothrida , R. cesticellus and R. ransomi with prevalence infection of 36.1% , 30.1% . 15.0 % and 1.8 % respectively . And the genus Choanotaenia . These species included C. infundibulum and C. passerine with pervatence infection of 15.0% and 0.6% respectively . And the genus Anonchotuenia . The species included A.globate with prevantence infection 1.2% .
... Show MoreThe current study aims to determine the prevalence of Trichomonas vaginalis and Candida spp., and also to identify Candida parapsilosis and some virulence genes. It was conducted in Bint Al-Hoda Hospital of Maternity and Children in Thi-Qar province, south of Iraq for the period from the beginning of January to the end of December 2020. Two hundred and fifty samples were collected from the female genital tract for women whose age ranged between 17-50 years. Microscopic, traditional and molecular tests were used in the sample examination. The results recorded 12 (4.8%) samples infected with T. vaginalis parasite, whereas 130 (52%) samples showed Candida yeast distributed as follows: 75 (30 %) <
... Show MoreExamining of passeriform birds collected in Baghdad area revealed presence of seven species of blood parasites belonging to three genera, Haemoproteus, Leucocytozoon, and Plasmodium. Records of microfilariae (larval nematodes) were also indicated. Results showed wide distribution of Plasmodium relictum among passerine hosts.
Objectives: To determine the level of knowledge regarding drugs addiction among paramedical staff; and to
identify the association between the level of knowledge and their socio-demographic variable.
Methodology: A cross-sectional study was Conducted in Baghdad on Staff for the period from 1st June,
2016 to30th September, 2016 . The sample included 510 Health professionals . There are 9 sectors in center
of Baghdad , 5 of them in Al- Kurkh side of the city and 4 in Al-Russafa side. The sample was selected from
49 primary health care centers and 8 public hospitals through a multi-stage sample using a simple random
sampling technique. The information was collected through the design of a questionnaire whose stability and<
Nosocomial infections (NIs) are hospital-acquired associated infections, and also contracted due to the infections or toxins that exist in some location, like hospital. Therefore in our study, 4 Lactic acid bacteria (LAB) isolates were obtained from dairy product (Lactobacillus brevis, L. acidophilus, Lactococcus raffinolactis and Lactococcus lactis) and were tested for Bacteriocin production to select Lactococcus lactis among them. Cell free supernatant (CFS), Lipid and partial purification of protein La. Lactis had high inhibitory effect against test pathogens (E. coli, Bacillus cereus, Staphylococcus aureus and Streptococcus). 30 isolates that diagnosed by Vitec, were isol
... Show MoreAsbestos is a hazard pollutant to human health, exposure to asbestos cause serious health effects and wide range of asbestos-related diseases such as asbestosis, lung cancer and malignant mesothelioma and it has been classified as carcinogen by the World Health Organization WHO which cause a carcinogenic effects. Fibers of asbestos are mainly released from friction product in brakes and clutch linings and from reinforce agent in the asbestos cement industry. The aim of this was to evaluate the levels of asbestos fibers in surroundings air of some dense traffic points in Baghdad, through winter 2020. Materials and Methods: Samples of airs was carried out by directing air flow to a mixed cellulose ester membrane filter mounted on an open face
... Show MoreGroundwater is considered as one of the most important sources of fresh-water, on which many regions around the world depend, especially in semi-arid and arid regions. Protecting and maintaining groundwater is a difficult process, but it is very important to maintain an important source of water. The current study aims to assess the susceptibility of groundwater to pollution using the DRASTIC model along with the GIS environments and its tool boxes. A vulnerability map was created by relying on data collected from 55 wells surveyed by the researchers as well as archived records from governmental institutions and some international organizations. The results indicate that the region falls into three vulnerability functional zones , namely
... Show MoreThe guava plant, Psidium guajava L., serves as proof of the abundant donations of nature, providing a delicious guava fruit; this plant is rich in groups of medicinal and nutritional benefits. Guava belonging to the Myrtaceae family, many previous studies reported many phytochemical constituents in its leaves that have many pharmacological activities and medicinal properties; this study focuses on the isolation, structural elucidation and calculation concentration of flavonoids, assessment of the cytotoxic activityof hyperin from Psidium guajava leaves newly cultivated in Iraq. The isolation process involved the use of thin-layer chromatography (TLC) and preparative high-performance liquid chromatography (PHPLC) and structural eluci
... Show MoreBackground: Reliable detection the etiological agent of amoebic dysentery and extra-intestinal amoebiasis have Public health importance specially in asymptomatic human and animals, Since the acquisition of pet dogs in the recent period has become widespread in our city. Aim: To give correct perception of infection rate in asymptomatic individuals (human and domestic dogs) for the first aspect and about detection and diagnosis of the pathogenic species of Entamoeba histolytica from another morphologically similar and commensal one using the molecular technique in stool samples of asymptomatic individuals the second aspect. Methods: During the study period from the beginning of September 2020 to the end of February 2021, a total of 95 stool s
... Show MoreBackground: Emergency ambulance personnel (EAP) are vulnerable to PTSD. In Iraq, EAPs were exposed to life threatening situation during political violence conflict post 2003 Gulf war. This study was carried out to report on PTSD among EAP.
Patients and Methods: A total of 189 male EAPs were selected randomly from the Dept. of ambulance in Baghdad. A questionnaire form was filled for each EAP. The form included the
symptoms of PTSD according to DSM- IV (intrusive, avoidance and hyperarousal symptoms). Demographic data and exposure to violence accident, type of accident and duration of damage were included, also.
Results: The mean age of EAP was 38.5 ± 9.4 years. There was 122 (64.5%) had PTSD. Age,