A simple, sensitive, accurate and economic spectrophotometric method has been developed for the determination of sulfacetamide (SFA) in pure form, synthetic sample and urine. The method is based on diazotization of primary amine group of sulfacetamide with sodium nitrite and hydrochloric acid followed by coupling with chromotropic acid in alkaline medium to obtain a stable orange colored chromogen which exhibit a maximum absorption (λmax) at   511.5 nm. Different variables affecting the completion of reaction have been carefully optimized following the classical univariate sequence and  modified simplex method (MSM). Under optimized conditions, Beer’s law obeyed in the concentration range of (0.5-  &nbs

The current study included bioremoval of chromium metal ions from aqueous solution by using seventeen Pseudomonas aeruginosa species isolated from different environments. The experimental results showed that isolates Pseudomonas aeruginosa have high efficiency in removal of chromium where the P. aeruginosa p.8 was the most efficient (P≥0.001) in bioremoval of chromium with a removal capacity reached 92.5 mg/L and removal index reached (96.5%). While P. aeruginosa p.4 was the least efficient (P≥0.001) in bioremoval of chromium from aqueous solutions reached 74.6 mg/L and removal index reached (79.8%). The REP-PCR detection using BOX-primer, showed genetic relatedness among the isolates of P.aeru

PvcABCD are cluster of genes found in Pseudomonas aeruginosa. The research was designed to examine the relationship between the pvc genes expression and cupB gene, which plays a crucial role in the development of biofilm, and rhlR, which regulates the expression of biofilm-related genes, and to investigate whether the pvc genes form one or two operons. The aims were achieved by employing qRT-PCR technique to measure the gene expression of genes of interest. It was found that out of 25 clinical isolates, 21 isolates were qualified as P.aeruginosa. Amongst, 18(85.7%) were evaluated as biofilm producers, 10 (47.6%), 5 (23.8%), and 3 (14.2%) were evaluated as strong, moderate and weak producers respectively, while, 3 (14.2%) were considered

The aim of this study is to investigate the role of prodigiosin on P. aeruginosa' s biofilm genes involved in the pathogenicity and persistency of the bacteria; Materials and methods: Gram negative bacterial isolates were taken from burn and wounds specimen obtained from some of Baghdad hospitals. Forty six isolates were identified as Pseudomonas aeruginosa and four isolates as Serratia marcescens by using biochemical tests and VITEK 2 compact system. Susceptibility test was performed for all P. aeruginosa isolates, the results showed that 100% were resistant to Amikacin and 98% were sensitive to Meropenem. Resistant isolates were tested for biofilm formation; the strong and moderate isolates (17) were detected by PCR for AlgD gene