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Assessment the Effect of Lactobacillus Acidophilus on Escherichia Coli Serotype O157:H7 with Detection of Some Virulence Factors
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Aim: To evaluation the effect of Lactobacillus acidophilus on Enterohemorrhagic Escherichia coli (EHEC) serotype O157:H7 with detection of some virulence factors. Methods: Two hundred and fifty specimens (stool) from children under five years for both sexes were collected from some hospitals. All isolates were diagnosed according to morphological characteristics, biochemical tests. Monoplex pattern of PCR was used also for detection different genes in (7) Escherichia coli )O157:H7 (isolates; include 16SrRNA, eae, lifA, Stx1,Stx2 that encoded for ribosomal RNA, intimin, lymphocyte inhibitory factor, shiga toxins. Three types of probiotics strains were obtained, Lactobacillus fermentum, Lactobacillus plantarum and Lactobacillus acidophilus (ATCC4356). Minimum inhibitory concentration (MIC) of cell free supernatants of Lactobacillus acidophilus was determined by employing different dilutions (1/2, 1/4, 1/8, 1/16, 1/32), to detect the concentration of probiotic that will inhibit E.coli (O157:H7) growth. Results: Results showed, 210 (84%) samples were identified as E.coli from 250 samples. The result showed (7) isolates were identified as Enterohemorrhagic Escherichia coli (EHEC) serotype O157:H7 and showed all isolates of O157:H7 were positive for 16SrRNA gene with (213bp) and eae with (741bp), lifA with (712bp), only Stx1 gene appeared in all isolates with (446bp) and no bands with Stx2. Current result showed only cell free culture supernatant of Lactobacillus acidophilus has inhibitory activity against all E.coli (O157:H7) isolates with different dilutions (1/2, 1/4, 1/8, 1/16, 1/32), while Lactobacillus fermentum, Lactobacillus plantarum have no effect against E.coli (O157:H7). The result showed the bacteriocin has inhibitory effect against E.coli (O157:H7), while organic acids and hydrogen peroxide haven’t any role in inhibition. The (MIC) value was (1/8) which inhibits the bacterial growth of isolates.

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Publication Date
Wed Feb 08 2023
Journal Name
Iraqi Journal Of Science
Molecular Detection of Klebsiella pneumoniae serotype K2 Isolated Clinically
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One hundred specimens (urine and sputum) were collected in sterilized containers from patients attending two hospitals in Baghdad province including: Educational Al-Karama Hospital and Educational Al-Yarmouk Hospital, in which, thirty-eight K. pneumoniae isolate were obtained out of one hundred specimens, all of which are of urine origin. The results obtained in this study showed that Klebsiella pneumoniae isolates were highly resistant to Augmentine (94.7%), also Klebsiella isolates were resistant to Ceftriaxone (86.8%), Cefotaxime (81.5%), and Ceftazidime (65.7%). Very low level of resistance was found toward Imipenem and Meropenem (18.4% and 5.2%) respectively. Only four isolates (10.52 %) were positive for wzy gene; K19, K20, K21, an

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Publication Date
Tue May 01 2018
Journal Name
Journal Of Physics: Conference Series
Pathological And Immunological Study On Infection With Escherichia Coli In ale BALB/c mice
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Publication Date
Wed Jun 01 2022
Journal Name
Baghdad Science Journal
Evaluation of some Virulence Factors and Drug Resistance of Bacteria Isolated from the Urine of Patients with TCC-Bladder Cancer
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Urinary tract infections (UTIs) mean microbial pathogens in the urethra or bladder (lower urinary tract). Important risk factors for recurrent UTI include obstruction of the urinary tract, use of a bladder catheter or a suppressed immune system. This study aims to isolate and identify bacteria from patients with TCC-bladder cancer or patients with a negative cystoscope and estimate antibiotic susceptibility patterns and evaluate some of the virulence factors. From a total of 62 patients with TCC-BC or negative cystoscope, only 35 favorable bacterial growths were obtained, including Escherichia coli (UPEC), a significant bacterial isolate, and Stenotrophomonas maltophilia. The percentage of multi drug-resistance bacteria

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Publication Date
Wed Dec 15 2010
Journal Name
Iraqi Journal Of Laser
Influence of 805 nm Diode Laser on Plasmid Content of Some Locally Isolated Escherichia coli and Proteus Mirabilis
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This work aimed to investigate the effect of Diode laser 805 nm on plasmid DNA and RNA
contents of some Gram negative bacteria represented by Escherichia coli and Proteus mirabilis isolates
.Plasmid extraction was done using two methods (Salting out and CTAB method).Different powers and
pulse repetition rates for 805 nm Diode Laser were used to study this effect. Results revealed that the
plasmid profile of the two species were highly affected using (2, 3) W at different frequencies including
5and 10 kHz as compared with 1 kHz while plasmids were gradually disappeared at 1W, 10 kHz. In the
same time the shining of RNA was also decreased gradually then disappeared with increasing powers
especially at 2W and 10 kHz cau

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Publication Date
Tue Mar 26 2019
Journal Name
Iraqi Journal Of Science
Biosynthesis of Iron Oxide Nanoparticles Using Escherichia coli
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This study proposed to synthesize iron oxide by biological method nanoparticles. The E.coli is used to reduce Ferric chloride salt into iron particles. The formation of iron oxide nanoparticle was initially monitored by visual observation and then characterized with the help of various characterization techniques such as Uv-vis spectroscopy, (AFM) and (FTIR) analysis, which revealed that the biosynthesized iron oxide nanoparticles were spherical within size 27.7 nm. Optimization of iron oxide nanoparticle biosynthesis by E.coli was performed for parameters (temperature and pH) and the results revealed that temperature 37°C and pH 5 were the optimum conditions for iron oxide nanoparticales biosynthesis by E.coli.<

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Publication Date
Fri Jun 24 2022
Journal Name
Iraqi Journal Of Science
A Study of the Effect of Some Growth Conditions on Inhibitory Activity of Lactobacillus delbrueckii and Lactobacillus fermentum to reduce Growth of E.coli
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هدفت هذه الد ا رسة لاختبار تاثير بعض ظروف النمو على الفاعلية التثبيطية لبكتيريا Lactobacillus delbrueckii و L.fermentum على نمو بكتيريا E.coli وقد دلت نتائج د ا رسة اختبار الفاعلية التثبيطية
للعزلتان البكتيريتان Lactobacillus delbrueckii L.fermentum , ضد العزلة البكترية E.coli المستخدمة في هذه الد ا رسة عند الت ا ركيز المختلفة 100,90,70,50) ( % بان عالق العزلتان البكتيريتان
Lactobacillus delbrueckii و L.fermentum عند التركيز % 100 اعطى اعلى فاعلية تثبيطية ضد
بكتريا E.coli

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Publication Date
Tue Sep 19 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Study of Effect of Lactobacillus gasseri Filtrate on Growth of Some Pathogenic Fungi
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The inhibitory effect concentrated filtrate of local Lactobaciilus

gasseri against Candida albicans and Trichophyton mentagrophytes, T  ruhrum  and Aspergillus  flavus which were isolated from infected cases  in human  beings, tested  using two methods  :well diffu  ion and

Mixed with the mold medium (Sabouraud agar).

The  results  showed  that the concentrated filtrate  of L. gasseri have

inhibitory effect against Cundida ulbicans, the inhibition  zone was 13 mm. As well  as, the concentrated  tiltrate inhibited, considerably Trichophyton   mentagrophytes,

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Publication Date
Wed May 10 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Turbidimetric Method for Measuring of Antibacterial Effect of 2-Methyl-4-(3,4 dihydroxy Phenyl)-Thiazole on the Growth Activity of Escherichia Coli and Pseudomonas Aeruginosa
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Thiazoles are heterocyclic nitrogenous compounds, which were given a great attention due to their antibacterial effect, antihypertensive, stimulating glucose absorption (like antibiotics), acts as cytoprotective agent. The present work is conducted to evaluate the degree of antibacterial activity on the growth activity of Escherichia Coli (E.Coli) and Pseudomonas aeruginosa (P. aeruginosa) by incubation with 2-methyl-4-(3,4 dihydroxy phenyl)-Thiazole compound (MDHPT). Optical density (O.B) was measured by turbidmetric method, any increase in O.D would represent the increase in the bacterial growth .The results showed that there was a 75.7 % inhibition when using 190.4 g/ml (MDHPT) solution and 64.3% was obtained when using concentr

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Publication Date
Sun Mar 07 2010
Journal Name
Baghdad Science Journal
Evaluation for the Effect of Heat Stable Enterotoxin (a) Produced by Enterotoxigenic Escherichia coli on Different Cancer Cells In Vitro
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This study was conducted for evaluating the cytotoxic effect of heat stable enterotoxin a (STa) produced by enterotoxigenic Escherichia coli on the proliferation of primary cancer cell cultures, obtained from tumor samples that were collected from (13) cancer patients and as follows: (five colon cancer patients, two bladder cancer patients, two breast cancer patients, two stomach cancer patients and two lung cancer patients), and on normal cell line (rat embryonic fibroblast / REF) (in vitro) with the use of different concentrations starting from (1) mg/ml and ending with (0.0002) mg/ml by making two fold serial dilutions by using the 96- well microtiter plate, and in comparison with negative (PBS) and positive (MMC, at concentration

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Publication Date
Sun Jul 29 2018
Journal Name
Iraqi Journal Of Science
Investigation of the presence of some virulence factors of the Streptococcus pneumoniae isolates among patients in Basra Governora
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Extensive studies have been conducted on the microbial properties of Streptococcus pneumoniae  all over the world ,but there are few studies in Iraq on the most important factors of virulence possessed by S.pneumoniae isolates found in Iraq , 195 of sputum specimens were collected from patients with pneumonia acquired from the community who were clinically diagnosed by specialized doctors depending on symptoms and  Radiography of Chest . Eighteen isolates of  S.pneumoniae were  diagnosed  by  special traditional methods that used in the phenotypic identification . All isolates 18 (100%) have been  given  positive results for  the optochin test , bile solubility test

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