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Nigella sativa has various pharmacological properties and has been used throughout history for a variety of reasons.
However, there is limited data about the effects of N. sativa (NS) on human cancer cells. This study aimed at observing
the roles of methanolic extract of N. sativa on apoptosis and autophagy pathway in the Human PC3 (prostate cancer) cell
line. The cell viability was checked by MTT assay. Clonogenic assay was performed to demonstrate clonogenicity and
Western blot was used to check caspase-3, TIGAR, p53, and LC3 protein expression. The results demonstrated that PC3
cell proliferation was inhibited, caspase-3 and p53 protein expression was induced, and LC3 protein expression was
modulated. The clonogenic assay showed that PC3
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