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The Deubiquitylase USP5 Knockdown Reduces Semliki Forest Virus Replication in HeLa Cells
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Semliki Forest Virus (SFV), a member of the Alphavirus genus in the Togaviridae family, is a small-enveloped, positive-sense single-stranded RNA (+ssRNA) virus. The virus is spread by mosquitos and can infect humans, resulting in mild febrile disease with symptoms that include fever, myalgia, arthralgia, persistent headaches and asthenia.  Virulent strains of SFV in mice cause lethal encephalitis by infecting neurons in the central nervous system. In on-going experiments in the research group using a focused siRNA screen we have investigated the role of deubiquitylases (DUBs) during SFV infection (as a model alphavirus) and monitored the effect of DUB depletion on cell viability after infection. We identified a group of DUBs that have a pro-viral effect. The DUB, USP5, from this screen was validated to determine its effect upon viral replication. Here, we show that depleted USP5 in HeLa cells resulted in SFV RNA and viral yield at 8 h post-infection being significantly reduced. In the multi-step viral growth curve assay, in the absence of USP5, similar yields of SFV were determined at 2 and 4 h post-infection. However, a significant reduction in the infectious viral particles release at 6, 8, 10 and 12 h post-infection was observed and this could be reversed by direct constraining viral replication. These results raise the potential for USP5 to play a distinct role in the replication of SFV, suggesting that USP5 may be a possible anti-viral therapy for alphavirus infection.

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Publication Date
Sat Jun 30 2012
Journal Name
Al-kindy College Medical Journal
Bone Cutter Circumcision in Neonates
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Background: Bone cutter is widely used in the
Middle east area to cut the prepuce during circumcision.

Results :One hundred twenty one male neonates
were included in this study.The mean operative
time (MOT) was 6 minutes, mean time of
healing (MTH) was 5 days, 7 cases (5.9%)
developed early & late complications, 2 (1.7%)
had bleeding, 3 (2.5%) developed infection, 2
(1.7%) developed late complications in the form
of meatal stenosis.
Settings:Al-Yarmouk Teaching Hospital and a
private surgical clinic.
Aim of the study: To assess the safety of
circumcision using bone cutter.
Methods: Over 6 years period from Jan. 2000
till Dec. 2005, one hundred twenty one male
neonates were circumci

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Publication Date
Wed Dec 14 2016
Journal Name
Journal Of Baghdad College Of Dentistry
Surgical Biopsy in Cervical Lymphadenopathy
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Background: Cervical lymph nodes are the most frequently enlarged and biopsied of all the peripheral lymph nodes and in most of the cases the enlargement results from benign infectious causes, however, the presence of cervical lymphadenopathy (LAP) requires accurate diagnosis to exclude more serious causes. The aim of this study was to analyze cases of Iraqi patients presenting with cervical LAP who underwent surgical lymph node biopsy to establish accurate diagnosis. Materials and Methods: This retrospective study included 25 patients who presented with cervical LAP for whom surgical biopsy was performed to establish a definitive diagnosis. The investigated data included the demographic and clinical parameters in addition to the final his

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Publication Date
Fri Aug 02 2024
Journal Name
Rafidian Journal
Quorum Sensing in Staphylococcus aureus
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Staphylococci are common commensals in human beings, yet certain species are pathogenic. Staphylococcus aureus, particularly, is a very virulent human pathogen. The capacity of staphylococci to sense the density of bacterial cell, i.e., quorum, and thereafter respond via genetic modifications is attributable to one primary mechanism known as accessory gene regulator (Agr). Agr's extracellular signal is a peptide that is posttranslationally modified with a thiolactone molecule. Agr is in charge of the upregulation of numerous exotoxins and hydrolyzing enzymes, as well as the downregulation of many colonization determinants, under circumstances of high cell density. This modulation is critical for the scheduling synthesis of virul

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Publication Date
Fri Jan 01 2021
Journal Name
Route Educational And Social Science Journal
CONSONANT CLUSTER IN KURDISH LANGUAGE
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Publication Date
Tue Jun 30 2015
Journal Name
College Of Islamic Sciences
Divine attributes In Islamic thought
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The subject of divine attributes is one of the most important Islamic tenets, which must be
To stand up to its accuracy and multiplicity unlike the difference of speech, which led the teams to disbelieve
The other, when they were engaged in the issue of divine attributes, but they are agreed and assembled on
That God is one in himself and his qualities and actions. It is not similar to Himself and does not have two qualities of sex
One . It has no similar or real similarity between its qualities and the qualities of the creatures, as well as the acts of God
God is one in His actions and does not share it with anyone, and they have based their consensus on belief
In the unity of God and the unification of Raboubiya and t

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Publication Date
Tue Feb 05 2019
Journal Name
Journal Of The College Of Education For Women
Sound Similar in Surat Poets
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This research deals with the phenomenon of a similar voice phenomenon has founded
its own laws similar and includes the following phenomenon:
Azahrh aledgam: and Qdbent Tarifaoshrooth or dinaces and reflects the correlation
between language and reading and then apply the provision in sura blessed.
Phenomenon Alaalal and substitution, Two of the important acoustic phenomenon the
linguistic heritage has shown Alaalal types and the difference between him and the
substitution then counted their applications in Sura.
Similar phenomena: in name and deed shares bult causes and probability voices and
sound that refrais where tilt.

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Publication Date
Tue Sep 01 2020
Journal Name
Baghdad Science Journal
Copulas in Classical Probability Sense
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               Copulas are simply equivalent structures to joint distribution functions. Then, we propose modified structures that depend on classical probability space and concepts with respect to copulas. Copulas have been presented in equivalent probability measure forms to the classical forms in order to examine any possible modern probabilistic relations. A probability of events was demonstrated as elements of copulas instead of random variables with a knowledge that each probability of an event belongs to [0,1]. Also, some probabilistic constructions have been shown within independent, and conditional probability concepts. A Bay's probability relation and its pro

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Publication Date
Sun Jun 01 2008
Journal Name
Baghdad Science Journal
Tamper Detection in Text Document
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Although text document images authentication is difficult due to the binary nature and clear separation between the background and foreground but it is getting higher demand for many applications. Most previous researches in this field depend on insertion watermark in the document, the drawback in these techniques lie in the fact that changing pixel values in a binary document could introduce irregularities that are very visually noticeable. In this paper, a new method is proposed for object-based text document authentication, in which I propose a different approach where a text document is signed by shifting individual words slightly left or right from their original positions to make the center of gravity for each line fall in with the m

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Publication Date
Wed Sep 04 2019
Journal Name
American Association Of Pharmaceutical Scientists
Recent advances in polymeric implants
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Implantable drug delivery systems, such as drug pumps and polymeric drug depots, have emerged as means of providing predetermined drug release profiles at the desired site of action. While initial implants aimed at providing an enduring drug supply, developments in polymer chemistry and pharmaceutical technology and the growing need for refined drug delivery patterns have prompted the design of sophisticated drug delivery implants such as on-demand drug-eluting implants and personalized 3D printed implants. The types of cargo loaded into these implants range from small drug molecules to hormones and even therapeutic cells. This review will shed light upon recent advances in materials and composites used for polymeric implant fabrication, hi

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Publication Date
Mon Jan 01 2018
Journal Name
دراسات في آثار الوطن العربي
Chemistry technology in ancient Mesopotamia
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