16S rRNA gene sequence examination is an effective instrument for characterization of new pathogens in clinical specimens. Akey component of colonization, biofilm formation, and protection of the pragmatic human pathogen Pseudomonasaeruginosais the biosynthesis of the exopolysaccharide Psl.Extracellular polysaccharides,biofilm, are secreted by microorganisms into the neighboring environment and are significant for surface attachment and keeping structural safety within biofilms.Biofilm production is an important technique for the survival of P. aeruginosa,and its association with antimicrobial resistance represents a defy for patient therapeutics. The aim of the current research is to assess the antibiotic resistance manner and distribution of the pslA gene among biofilm producingP. aeruginosa isolates, which have beengained from some hospitals in Baghdad, Iraq. Twenty-five P. aeruginosa isolates were obtained fromDepartment of Biology, College of Science, University of Baghdad. TheP. aeruginosa isolates were recognized using standard bacteriological techniques. Drug susceptibility test was done by disk diffusion technique for all the isolates against five antimicrobial agents.DNA was extracted from twenty-fiveP. aeruginosa isolates, which were selected as being resistant to gentamicin using the polymerase chain reaction(PCR). A specific primer pair was used to amplify 16S rRNA by a conventional PCR technique. Biofilm development was measured by microtiter plate test. The results of 16S rRNA showed that all 25 selected isolates were resistant to gentamicin harbored this gene. Biofilm formation was observed in 24/25(96%) of the P. aeruginosa isolates. The possibility of biofilm formation was remarkablyrelatedtothe resistance to gentamicin. In addition, the pslA gene was existed in all biofilm and non-biofilm producing the selected isolates with a frequency of 100% (n = 25).16S rRNA sequencing can be used to identify genetically atypical P. aeruginosa isolates from different origins. Theresults of the currentresearch well clarified that the P. aeruginosa biofilm-forming isolates were more resistant to the tested antibiotics. What is more, because of wide spreading, it appears that the pslA gene is associated with biofilm formation.
In this paper, we derived an estimators and parameters of Reliability and Hazard function of new mix distribution ( Rayleigh- Logarithmic) with two parameters and increasing failure rate using Bayes Method with Square Error Loss function and Jeffery and conditional probability random variable of observation. The main objective of this study is to find the efficiency of the derived of Bayesian estimator compared to the to the Maximum Likelihood of this function using Simulation technique by Monte Carlo method under different Rayleigh- Logarithmic parameter and sample sizes. The consequences have shown that Bayes estimator has been more efficient than the maximum likelihood estimator in all sample sizes with application
In this research, the focus was on estimating the parameters on (min- Gumbel distribution), using the maximum likelihood method and the Bayes method. The genetic algorithmmethod was employed in estimating the parameters of the maximum likelihood method as well as the Bayes method. The comparison was made using the mean error squares (MSE), where the best estimator is the one who has the least mean squared error. It was noted that the best estimator was (BLG_GE).
This study focuses on the basics of cultivation and production of the Wheat Crop in
Wasit Province ; in order to reveal the reality of the geographical distribution of its cultivation
and production.
Wheat is the most important field crops cultivated in Wasit province, as it has vast
possibilities for expansion in growing and production, in which agriculture conducted in a
year and left in other , then changing this method would increase cultivated area of it to nearly
doubled, as well as the possibility of increasing average yields in Donam by using improved
seeds, fertilizer, with the availability of water lever machines, especially wheat is grown in
areas of rivers abutment.
The study showed the spread of th
Anthropogenic activities cause soil pollution with different serious pollutants, such as polycyclic aromatic hydrocarbon (PAHs) compounds. This study assessed the contamination of PAHs in soil samples collected from 30 sites divided into eight groups (residential areas, oil areas, agricultural areas, roads, petrol stations, power plants, public parks and electrical generators) in Basrah city-Iraq during 2019-2020. The soil characteristics including (moisture, pH, EC and TOC) were measured. Results showed the following ranges (soil moisture (0.03-0.18%),pH (6.90-8.16), EC (2.48-104.80) mS/cm and TOC (9.90-20.50%)). Gas Chromatography (GC) was used to measure PAHs in extracted soil samples. The total PAH range (499.96 - 5864.86) ng/g dr
... Show MoreThis paper is concerned with preliminary test double stage shrinkage estimators to estimate the variance (s2) of normal distribution when a prior estimate of the actual value (s2) is a available when the mean is unknown , using specifying shrinkage weight factors y(×) in addition to pre-test region (R).
Expressions for the Bias, Mean squared error [MSE (×)], Relative Efficiency [R.EFF (×)], Expected sample size [E(n/s2)] and percentage of overall sample saved of proposed estimator were derived. Numerical results (using MathCAD program) and conclusions are drawn about selection of different constants including in the me
... Show MoreSingle Point Incremental Forming (SPIF) is a forming technique of sheet material based on layered manufacturing principles. The sheet part is locally deformed through horizontal slices. The moving locus of forming tool (called as toolpath) in these slices constructed to the finished part was performed by the CNC technology. The toolpath was created directly from CAD model of final product. The forming tool is a Ball-end forming tool, which was moved along the toolpath while the edges of sheet material were clamped rigidly on fixture.
This paper presented an investigation study of thinning distribution of a conical shapes carried out by incremental forming and the validation of finite element method to evaluate the limits of the p
... Show MoreThis paper aims to decide the best parameter estimation methods for the parameters of the Gumbel type-I distribution under the type-II censorship scheme. For this purpose, classical and Bayesian parameter estimation procedures are considered. The maximum likelihood estimators are used for the classical parameter estimation procedure. The asymptotic distributions of these estimators are also derived. It is not possible to obtain explicit solutions of Bayesian estimators. Therefore, Markov Chain Monte Carlo, and Lindley techniques are taken into account to estimate the unknown parameters. In Bayesian analysis, it is very important to determine an appropriate combination of a prior distribution and a loss function. Therefore, two different
... Show MoreIn this study, we used Bayesian method to estimate scale parameter for the normal distribution. By considering three different prior distributions such as the square root inverted gamma (SRIG) distribution and the non-informative prior distribution and the natural conjugate family of priors. The Bayesian estimation based on squared error loss function, and compared it with the classical estimation methods to estimate the scale parameter for the normal distribution, such as the maximum likelihood estimation and th
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