سلوى علي غانم
-
Salwa Ali Ghanim
MSc
-
assistant lecturer
College of Education for Pure Sciences (Ibn Al-Haitham)
,
Deparment of Biology
[email protected]
Publication Date
Mon Apr 10 2017
Genetic variation between wild type and auxotrophic mutant isolates of Sinorhizobium meliloti by using RAPDPCR technique
Genetic variation
Sinorhizobium meliloti
RAPD
Nitrous acid
SGI2 wild type isolate of Sinorhizobium meliloti was isolated from Medicago sativa (alfalfa) plant which was obtained from Al-Tarmiaa region / Baghdad. Nine auxotrophic mutants were obtained from the SGI2 wild type isolate by mutagenesis with Nitrous acid (HNO2). The SGI2 wild type and the all auxotrophic mutant isolates had two Megaplasmids; pSymA and pSymB. No genetic variations in plasmid number and size were detected when gel electrophoresis was done for plasmid profile detection. Genetic variations by using RAPD-PCR technique were obtained between wild type and auxotrophic mutant isolates. One band was detected in SGI6 gel profile with 1.5 Kb size when OPY-04 primer was used. Using OPB7 primer by using RAPD-PCR technique showed larg
... Show MoreNo Events Found