Researcher Image
علي صباح علي الحسني - Ali Sabah Ali Al-Hassani
PhD - professor
College of Agricultural Engineering Sciences , Department of Animal Production
[email protected]
Summary

Ali S. Al-Hassani received his M.Sc. degree from the University of Baghdad, a college of agricultural sciences in the Department of Animal Resources, in 2007 in the use of local and impourant vatamin and salats to elevate heat stress side effects in broiler chickens in cooperation with the RCI company for the chimical industry in Australia. Also, he received his Ph.D. from the same college in 2015 in avian physiology and molecular biology.

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Qualifications

15 years of experience in teaching avian physiology and molecular biology at the University of Baghdad; 5 years of experience as a manager of sheep and layer chicken farms in the Al-Rafidain Company for animal production in Iraq.

Responsibility
  1. Teaching in the Department of animal production from 2008 to present
  2. Manager of the internet and informatics unit at the college of Agriculture from 2010–2014
  3. Manager of the website unit at the college of Agriculture, 2013-2015
  4. Manager of the follow-up and coordination division at the College of Agriculture from 2015–2021.
Awards and Memberships
  1. Bronze medal at the third international exhibition of inventions held in Kuwait (2010)
  2. Silver medal at the seventh international exhibition of inventions held in Kuwait 2014
  3. Silver medal and diploma certificate at the IV International Exhibition of inventions held in the Islamic Republic of Iran /Shiraz city 2014.
  4. Gold medal and certificate of appreciation at the International Exhibition of inventions and trade held in London, 2019
  5. Silver medal and certificate of appreciation at the International Exhibition of Inventions, held in the German city of Nuremberg 2019
Research Interests

Avian Physiology, Avian Molecular Biology, and Avian heat stress

Academic Area

Avian Physiology and Avian Moleculer Biology

Teaching materials
Material
College
Department
Stage
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فسلجة طيور داجنة
كلية علوم الهندسة الزراعية
الانتاج الحيواني
Stage 3
تقنات انتاج حيواني
كلية علوم الهندسة الزراعية
البستنة وهندسة الحدائق
Stage 2
فسلجة بيئة طيور داجنة متقدم لطلبة الدراسات العليا
كلية علوم الهندسة الزراعية
الانتاج الحيواني
Stage 6
منهجية البحث العلمي لطلبة الماجستير
كلية علوم الهندسة الزراعية
الانتاج الحيواني
Stage 5
Teaching
  1. Advanced scientific research methods, Animal Production Department for Master students
  2. Advanced avian environmental physiology, Animal Production Department or Master students
  3. avian physiology for 3rd stage student at department of animal production
  4. technical aspects of animal production for 2 end-stage students at the department of holticultare.
Supervision

Dr.Ali S.Al-Hassani supervised for 2 Masters and 1 Phd studnts

Publication Date
Sat Apr 01 2023
Journal Name
Iop Conference Series: Earth And Environmental Science
Effect of Ginger Powder Addition in Broiler Chicken Diets on Productive Performance

The purpose of this research was to examine the impact of varying doses of ginger powder on broiler productivity. There were 180 commercial strain Ross 308 day old chicks utilized in the experiment, and each chick was weighed and allocated randomly among four treatments. Three different treatments were tested, each with a replication of 15 chicks. The diet was supplemented with 0.5, 1.0 and 2.0g/kg of ginger powder, respectively, as a control. As compared to the other treatments, the control group had considerably higher body weights, weight growth, feed intake, and feed conversion ratio. Ginger powder use was shown to have a detrimental impact on growth in this investigation.

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Publication Date
Thu Jun 15 2023
Journal Name
Jornal Of Al-muthanna For Agricultural Sciences
Effect of different levels of Adrenocorticotropic hormone (ACTH) on physiological performance of broiler under heat stress conditions.

This experiment was holdup in A-Faris poultry farms from 1st March to 11 of Aprile 2019. (ACTH) hormone infusion was tested in this experiment on acid-base regulation in broiler chickens. For 7 days, osmotic pumps dispensed 8 IU of ACTH in saline/kg of BW/d, or the same volume of saline as in ACTH at 1 l/h. On days 0 and 14, after the beginning of the infusions, blood samples were obtained to establish a baseline. The plasma concentrations of Na+, K+, and Cl- were decreased, whereas the partial pressure of CO2, anion gap, corticosterone, mean corpuscular hemoglobin concentration, and blood concentrations of hemoglobin and HCO - were all elevated due to the ACTH administration. When given ACTH, neither blood pH nor plasma Ca2+ levels changed

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Publication Date
Sat Apr 01 2023
Journal Name
Iop Conference Series: Earth And Environmental Science
GH and IGF-2 Histological Effects on the Heart, Liver and Gizzard of Chickens were Studied

GH and IGF-2 were examined histologically in the present study on adult hens to learn more about the organs’ responses to GH and IGF-2. Cardiac protein synthesis is stimulated by GH and IGF-2, according to microscopic examination. The recent research found a considerable amount of adipose tissue in the cardiac muscle bundles, which is linked to the metabolic process. In addition, GH and IGF-2 were shown to promote protein synthesis and mitosis in liver and gizzard tissues, according to the research. In addition, the apoptosis, regeneration, and secretory activity of gizzard glands are increased by the aforementioned hormones.

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Publication Date
Wed Jan 11 2023
Journal Name
J. Of Kirkuk Univ. For Agri. Sci.
THE HARMFUL CONSEQUENCES OF HEAT STRESS ON CHICKENS INDUSTRY

This article dealt with identifying the importance of heat stress in poultry flocks and the damages resulting from the production of eggs and meat and the spread of pathogens, in addition to that ways to mitigate the harmful effects of heat stress, including physiological, nutritional, engineering and genetics, in order to ensure preservation of the product and reduce damages

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Publication Date
Thu Dec 29 2022
EFFECT OF DIETARY SUPPLEMENTATION WITH CLOMIPHENE CITRATE ON SOME PRODUCTIVE AND PHYSIOLOGICAL TRAITS OF LAYING JAPANESE QUAIL

This study was conducted to evaluate the effect of supplementation different levels of Clomiphene citrate to the diet of female quail on productive and some physiological traits. The experiment was conducted in a commercial farm from 2/3/2021 to 24/5/2021 (3 months). Females aged 50 weeks (160 females) were randomly distributed into four treatments, and each treatment included four replicates (10 birds for each replicate). The treatments were divided into a control group (T1; basic diet only), second treatment (T2; basic diet + 0.25 mg / female / day), third treatment (T3; basic diet + 0.50 mg / female / day, fourth treatment (T4

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Publication Date
Tue Oct 25 2022
BROILER GENOTYPING AND ALLILIC FREQUNCY ANALYSIS BY PCR-RFLP FOR IGF1 AND IGF2 GENES

This study was aimed to investigate Broiler chickens using PCR-RFLP (IGF1 and IGF2 genes). HinfI and Hsp92II restriction enzymes were used to amplify and digest 793-bp and 1146-bp fragments of the IGF1 gene, respectively. TT, TC, and CC genotypes were found in both genes in two different T and C allele subgroups. IGF1 had a higher prevalence of the C-allele (0.86) than IGF2, whereas IGF2 had a higher prevalence of the T-allele (0.5611).

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Publication Date
Tue Aug 30 2022
EFFECT DIFFERENT LEVELS OF TURMERIC ROOT POWDER TO DIET ON SOME TRAITS OF BROILER EXPOSED TO HEAT STRESS

This study was carried out in the poultry field in Jadiriyah, affiliated with the Department of Animal Production / College of Agricultural Engineering Sciences - University of Baghdad. This experiment used 480 one-day-old, unsexed broiler chicks of the broiler breed (Ross-308) with an average weight of 39.8 g to investigate the effect of adding different levels of turmeric root (CURCUMA LONGA) (CURCUMA LONGA) powder to the diet on some characteristics and physiology of commercial broilers exposed to heat stress. Chicks were distributed randomly and equally between 4 treatments (120 chicks/treatment), and each treatment included 3 replicates, at a rate of (40 chicks/repeat). Chicks were fed on the starter, growth and fin

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Publication Date
Wed Jan 05 2022
Journal Name
International Journal Of Aquatic Science
Effects of Selenium Sources and Concentrations on the Performance, Meat Quality, and Tissue Properties of Broiler Chickens

We wanted to find out how selenium (Se) affects broiler chicken performance, meat physicochemical properties, and selenium deposition in the tissues of broilers. Each of the 96 experimental pens had 30 chickens and included a total of 2,880 one-day-old broilers (Cobb 500 strain). A factorial design of four-by-three (SY + SS) and eight replicates (SY + SS) was used for the 12 experimental treatments, with selenium levels ranging from 0.15 to 0.60 ppm and organic (SY) or inorganic (SS) sources of selenium and their relationship (SY + SS). There were no differences in performance (P > 0.05) across Se levels or sources. 106 g/day of ADFI, 63 g/day of ADG, and 1.6844 kg/kg of FCR were found to be the averaging values for these three parameters:

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Publication Date
Tue Oct 25 2022
INSULIN LIKE GROWTH FACTOR (IGF-2) GENE POLYMORPHISMS INFLUENCES CERTAIN BIOCHEMICAL PARAMETERS IN BROILER CHICKENS

This study aimed to investigate genetic variations of the IGF-2 gene and how these polymorphisms affect different biochemical markers was conducted in this study. Broiler chicks of 300 days of age were analyzed in this research (150 Cobb500 and 150 Hubbard F-15). Each bird's blood was used to get its unique DNA sequence. Multiple biochemical features were connected to IGF-2 genotypes found using PCR-RFLP. If you're looking for an easy way to raise your chickens' cholesterol levels, go no further than Cobb500 chickens with the "TT" genotype (p≤0.05). Hubbard F-15 broilers with the TT genotype exhibited substantially higher levels of LDL (p≤0.05) than those with the TC and CC genotypes. The LDL levels of Hubbard F-15 broilers w

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Publication Date
Sat Dec 01 2018
EVALUTION OF CONTAMINATION STATUSE IN IMPORTED AND LOCAL TABLE EGGS

This study was conducted to detect the contamination in the local and imported table eggs, and the comparison between them of contamination.  Samples were collected from local markets   taking 60 samples of both types (30 local eggs and 30 imported eggs) these samples were sent to a laboratory of microbiology / College of Veterinary Medicine / University of Baghdad .then taking swabs from the   different of eggs shells samples for both types (local and imported eggs) then bot swabs in nutrient broth to be grown bacteria in the nutrient broth). (After that samples were taken from the nutrient broth) and then cultured in the (nutrient agar, macconky agar). The results showed the presence of

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Publication Date
Tue Apr 03 2018
Journal Name
Journal Of Entomology And Zoology Studies
Histological effects of growth hormone (GH) and Insulin-like growth factor-1 (IGF-1) on heart, liner and gizzard in chickens

In the present study, histological study was carried out on adult chickens to shed light on the effects of GH and IGF-1 on the heart, liver, and gizzard. The microscopic examination had shown that GH and IGF1 promote protein synthesis in the heart tissue. The herein work referred to the presence of a considerable amount of adipose tissue among the bundles of cardiac muscles, which is related to the metabolic process. The results also revealed that GH and IGF-1 promote both protein synthesis and Mitosis in the tissues of the liver and gizzard Moreover, the above hormones stimulate apoptosis, regeneration and secretory activity in gizzard secretory glands

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Publication Date
Tue Sep 15 2015
Journal Name
Asian Journal Of Poultry Science
Association of Insulin-Like Growth Factor-1 Gene Polymorphism at 279 Position of the 5’UTR Region with Body Weight Traits in Broiler Chicken

insulin-like Growth Factor 1 (IGF-1) gene has been described in several studies as a candidate gene for growth. The present study attempts to identify associations between body weight traits and polymorphisms at 279 position of 5'UTR flanking region of IGF-1 gene in broiler chickens. Three hundred broiler chickens from two breeds (Cobb 500 and Hubbard F-15) were used in this study. A single nucleotide polymorphism (SNP) at 279 position of 5'UTR region of the IGF-1 gene was identified in 20.6 and 60.3% of Cobb 500 and Hubbard F-15, respectively, using the PCR-RFLP technique. Allele frequencies were 83.87 and 42.80% for the T allele and 16.13 and 57.20% for the C allele in Cobb500 and Hubbard-15 breeds, respectively. Genotype frequencies were

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Publication Date
Thu Oct 12 2017
THE EFFECT OF IGF-1 GENE POLYMORPHISMS ON SOME PHYSIOLOGICAL AND PRODUCTIVE PARAMETRS IN BROILER CHICKEN BREED COBB500.

This experiment investigated the association between SNP 5 UTR region of insulin –like growth factor-1 ( IGF-1 ) and phenotypic productive  and physiological traits in cobb500 broiler breed .The study was conducted at poultry farm in Abu-Graib region  during a period from 1Nov 2016-14Dec 2016 .Four hundred fifty broiler chicks one day old (cobb500) were wing –banded and divided to three treatment groups (three replicates each) according to gene polymorphisms .DNA was extracted from fresh blood samples .Restriction fragment length polymorphism was used in the 5 UTR  region of IGF-1. Chicks were reared under conventional conditions according the guide and fed ad libitum at the end of experiment number of

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Publication Date
Wed Jun 13 2012
Journal Name
South African Journal Of Animal Science
Effect of <i>in ovo</i> injection with L-arginine on productive and physiological traits of Japanese quail

This study evaluated the influence of administering different levels of L-arginine into the eggs of 0-day-old Japanese quail embryos. On day 0 of incubation, 480 eggs (120 for each treatment group) were injected with 0% arginine (C group), 1% arginine (T1), 2% arginine (T2), or 3% arginine (T3). After hatching, 336 quail chicks (84 chicks produced from each ovo injection treatment) were placed in an experimental quail house and allocated to four treatment groups of three replicates, with 16 quail chicks for each replicate. Traits involved in this study were hatchability rate, initial body weight (7 days of age), final body weight (42 days old), feed intake, weight gain, feed conversion ratio, blood serum glucose, protein, cholesterol, total

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Publication Date
Sun Aug 05 2018
Journal Name
Journal Of Entomology And Zoology Studies
Some biochemical traits in broiler chicken as affected by insulin-like growth factor-1 (IGF-1) gene polymorphisms

This study was conducted during the period 1/9/2014 – 1/2/2015 and aimed to identify the polymorphisms of IGF-1 gene in broiler chickens and their effects on some biochemical traits. A total of 300 one-day-old broiler chicks (Cobb500, n=150; Hubbard F-15, n=150) were evaluated in this study. Blood samples were individually collected from all birds for DNA extraction. PCR-RFLP method being used for determination the genotypes of IGF-1 gene which then correlated with biochemical traits studied. Cobb500 broilers with TT genotype had significantly (p˂0.05) higher serum triglycerides values than those of TC and CC genotypes. Low density lipoprotein (LDL) were significantly (p˂0.05) higher in Hubbard F-15 broilers with TT genotype than those

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Publication Date
Thu Jan 05 2012
Journal Name
International Journal Of Scientific Research
The Influence of Parsley ( Petroselinum crispum ) as Feed Additive on Hematological Traits of Local Iraqi Geese

This study was conducted to investigate the effect of feeding diets containing different levels of parsley on the hematological traits of local Iraqi geese. A total of twenty-four local geese, one year old, were used in this experiment. The birds were allocated into four treatment groups, consisting of six geese each. Treatment groups were: control diet (C) (free from parsley); T1: control diet + 80 g/d parsley; T2: control diet + 160 g/d parsley; and T3: control diet + 240 g/d parsley. At the end of the experiment, blood samples were obtained from all geese from the brachial vein by venipuncture. Hematological traits included in this study were red blood cells count (RBC), hemoglobin concentration (Hb), packed cell volume (PCV), mean cell

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Publication Date
Sat Dec 15 2012
Journal Name
International Journal Of Applied Biology And Pharmaceutical Technology
EFFECT OF THE SUPPLEMENTATION OF THE LAYING QUAILS RATION WITHSESAME (SESAMUM INDICUM) SEEDS AND OIL ON EGG QUALITY TRAITS

The aim of this study was to determine the influence of feeding diets containing different levels of sesame seeds and oil on the egg quality of laying quail. A total of 120, 10 weeks old, were randomly assigned to 1 of 5 dietary groups and fed for 12 weeks diets containing 0% sesame seeds + 0% sesame oil (control group; C) or 0.5% sesame oil (T1), 1% sesame oil (T2), 1% sesame seeds (T3), and 2% sesame seeds (T4).The study was terminated when the birds were 22 weeks of age. Egg quality characteristics involved in the present study were egg weight, yolk diameter, yolk height, yolk weight, albumen height, albumen weight,Haugh unit, shell weight, shell thickness, shell percentage, yolk percentage, and albumen percentage. The addition of sesame

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Publication Date
Wed Jun 13 2012
Journal Name
South African Journal Of Animal Science
Effect of in ovo injection with L-arginine on productive and physiological traits of Japanese quail

This study evaluated the influence of administering different levels of L-arginine into eggs of 0-day-old Japanese quail embryos. On day 0 of incubation, 480 eggs (120 for each treatment group) were injected with 0% arginine (C group), 1% arginine (T1), 2% arginine (T2) or 3% arginine (T3). After hatching, 336 quail chicks (84 chicks produced from each in ovo injection treatment) were placed in an experimental quail house and allocated to four treatment groups of three replicates, with 16 quail chicks for each replicate. Traits involved in this study were hatchability rate, initial body weight (7 days of age), final body weight (42 days old), feed intake, weight gain, feed conversion ratio and blood serum glucose, protein, cholesterol, tota

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Publication Date
Sat Aug 25 2012
Journal Name
Journal Of American Science
The effect of utilization of parsley (Petroselinum crispum) in local Iraqi geese diets on blood biochemistry

This experiment was conducted to study the effect of feeding diets containing different levels of parsley on the blood biochemical characteristics of local Iraqi geese. A total of twenty-four local geese, one year old, were used in this experiment during the period from the beginning of October to the end of December. The birds were allocated for Four treatment groups consisted of six geese each. Treatment groups were as follows: Control diet (T1) (free from parsley), T2: Control diet + 80 g / d parsley, T3: Control diet + 160 g / d parsley; T4: Control diet + 240 g / d parsley. At the end of experiment, blood samples were obtained from all geese by venipuncture from brachial vein and blood plasma samples were prepared. Blood biochemical tr

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Publication Date
Wed Jul 11 2012
Journal Name
Euphrates Journal Of Agriculture Science
Effect of feeding diet containing flax seed on certain egg quality traits of Japanese quail

This experiment was performed to investigate the effect of dietary supplementation with flax seed on the egg quality of laying quail. A total of 320 Japanese quail (Coturnix coturnix japonica) wk old were allocated to 4 treatment groups with 4 replicates containing 20 quail each. Birds were fed a commercial diet containing 0% (C), 2% (T1), 4% (T2), or 6% (T3) flax seed. Birds received water and diet ad libitum during the total period of the experiment. Egg quality characteristics were monitored over 3 consecutive 21-day periods. Egg quality criteria involved in this experiment involved egg weight, yolk diameter, yolk height, yolk weight, albumen height, albumen weight, shell weight, shell thickness, Haugh unit, albumen percentage, yolk perc

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Publication Date
Tue Mar 08 2011
Journal Name
Research Opinions In Animal & Veterinary Sciences
Effect of dietary parsley (Petroselinum crispum) supplementation on semen quality of local Iraqi ganders

This study was carried out to investigate the effect of dietary supplementation with different levels of parsley on semen quality of local Iraqi ganders. A total of thirty-two local ganders were used in this study during the period from the beginning of February to the end of April. The ganders were allocated for 4 treatment groups containing 8 ganders each. The treatment groups were as follows: Control diet (free from parsley); T1: Control diet + 80 g/d parsley; T2: Control diet + 160 g/d parsley; T3: Control diet + 240 g/d parsley. Semen samples were collected twice a week, fortnightly, from each gander by the dorsal-abdominal message method. The first semen collection was used to evaluate semen volume, sperm concentration, live in total

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Publication Date
Mon Aug 15 2011
Journal Name
International Journal Of Poultry Science
Effect of Dietary Supplementation with Different Levels of Arginine on Some Blood Traits of Laying Hens

This experiment was carried out at the Field of Poultry, Department of Animal Resources, College of Agriculture, University of Baghdad, during the period from 1/5/2011 until 5/7/2011 to study the effect of adding arginine to laying hens diet on certain blood traits. A total of 100 Brown Lohmann laying hen chickens, 38 weeks of age, were randomly distributed into four treatment groups, with 25 hens for each treatment. Treatment groups were: T1: bird-fed diet with no additional arginine (control group); T2, T3, and T4: bird-fed diet supplemented with 0.4, 0.7, and 0.9%, respectively. Therefore, the total amounts of arginine in the four treatments (T1, T2, T3, and T4) become 1.1, 1.5, 1.8, and 2.0%, respectively. Results of this experiment rev

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Publication Date
Thu Jan 06 2011
Journal Name
Arpn Journal Of Agricultural And Biological Science
EFFECT OF FEEDS CONTAINING DIFFERENT FATS ON CERTAIN CARCASS PARAMETERS OF JAPANESE QUAIL

The aim of this study was to determine the effect of different dietary fat on carcass traits in Japanese quail (Coturnix coturnix japonica). A total of 168 7-week-old Japanese quail (48 males and 120 females) were used in this study. They were evenly distributed into 4 treatment groups, with 3 replicates per group containing 4 males and 10 females each. The birds were fed a basal diet with different fats (sunflower, flax, corn, and fish oils) at a 3% level for 13 weeks. On the last day of the experiment, 12 birds were randomly selected from each treatment group (6 males and 6 females) and slaughtered to determine carcass characteristics included in this study, which were carcass weight, dressing percentage with or without giblets, and the r

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Publication Date
Thu Apr 15 2010
Journal Name
International Journal Of Poultry Science
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Publication Date
Sat Jan 15 2011
Journal Name
International Journal Of Poultry Science
Influence of Source of Oil Added to Diet on Egg Quality Traits of Laying Quail

This experiment was performed to investigate the influence of different oils in the diets of laying quail on their egg quality characteristics. One hundred and twenty 7-week-old Japanese quails (Coturnix coturnix japonica) were allocated to four groups with three replicates containing 10 quail each (30 quail per treatment group). They were fed for 13 weeks (including one week of adaptation period) on diets containing 3% oil from different sources, viz., sunflower (T1), linseed (T2), maize (T3), or fish oil (T4). Inclusion of the diet of laying quail with fish oil (T4) and maize oil (T2) resulted in a significant increase with respect to egg weight, yolk weight, albumen weight, yolk diameter, yolk height, albumen diameter, albumen height, sh

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Publication Date
Sat May 15 2010
Journal Name
International Journal Of Poultry Science
Effect of Dietary Linseed on Egg Quality of Laying Quail

To assess the effects of dietary oil sources on productive and reproductive traits, sunflower oil, flax oil, corn oil, or fish oil were induced in quail diets. One hundred and sixty-eight 7-week-old Japanese quail were randomly assigned to 4 groups (12 males and 30 females each) with 3 replicates per group containing 4 males and 10 females each and fed for 13 weeks (including one week as an adaptation period) on a commercial diet supplemented with 3% of sunflower oil (T1), flax oil (T2), corn oil (T3), or fish oil (T4). The birds received water and were fed ad libitum during the study. The results of the experiment revealed that dietary supplementation with different sources of oil had no significant effect on male body weight, female body

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Publication Date
Sat Mar 15 2008
Journal Name
فسلجة دم الطيور
فسلجة دم الطيور

يعّد علم فسلجة دم الطيور Hematology Avian أحد العلوم الرئيسية المهمة ضمن علم فسلجة الطيور Physiology Avian. وتكمن أهمية هذا العلم في كونه العنصر الأساس لتقييم الصحة العامة والحيوية والأداء الفسلجي والإنتاجي للطيور. ولا يمكن الحكـم بـصورة قطعية على صحة وحيوية قطيع ما من الطيور من غير تقييم صورة الدم العامة لهذا القطيع. وبالنظر لعدم وجود أي مصدر عربي يتعلق بفسلجة دم الطيور ولندرة المصادر العالمية وعدم توفرها في المكتبات المح

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Publication Date
Tue Jun 15 2010
Journal Name
International Journal Of Poultry Science
Effect of Dietary Supplementation with Sources of Omega-3 and Omega-6 Fatty Acids on Certain Blood Characteristics of Laying Quail

The objective of this experiment was to determine the effects of dietary supplementation with different fat sources on blood parameters of Japanese quail (Coturnix coturnix japonica). Eighty four 7-week old laying quail were randomly assigned to 4 treatment groups (21 birds per group) with 3 replicates for each treatment group and fed for three months on a commercial diet supplemented with 3% of either sunflower oil (T1), flax oil (T2), corn oil (T3) or fish oil (T4). The birds received water and feed ad libitum during the experiment. During the last month of experiment blood samples were collected fortnightly from each bird. The first blood samples collection was used to determine fresh blood parameters, while the second blood samples coll

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Publication Date
Tue Jun 15 2010
Journal Name
International Journal Of Poultry Science
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Publication Date
Tue Jan 24 2023
Journal Name
Euphrates Journal Of Agricultural Science
PCR-RFLP Analysis of Insulin-Like Growth Factor 2 Gene Polymorphisms in Two Commercial Broiler Chicken Strains (Cobb 500 and Hubbard F-15) and Their Associations with Performance Traits

In two commercial broiler breeds (Cobb 500 and Hubbard F-15), the polymorphisms of the chicken insulin-like growth factor 2 (IGF2) gene were studied. A total of three hundred avian blood samples were obtained. Using a fast salt-extraction technique, genomic DNA was isolated. Using polymerase chain reaction, 1146 bp fragments of the gene were amplified (PCR). The amplified fragments were subjected to restriction enzyme digestion using HinfI endonuclease enzyme, and the digested products were separated on a 2% agarose gel. The findings indicated two alleles T and C for the target locus, with respective frequencies of 73.3% and 26.7%. Three distinct genotype variations, TT, TC, and CC, were found, with genotype frequencies of 59.1 percent, 28.

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