One hundred sheep with bilateral otitis externa were studied one year and a half .The
exudates of both external ears obtained using sterile swabs, and microorganisms were isolated
according to standard microbiological techniques. There was bacterial and fungal growth in
many of the samples. The total numbers of isolates from right ear was 104 , and the most
common pathogens isolated in right ear were Staphylococcus aureus 30.76 % , then
Staphylococcus epidermidis 9.61% and Pseudomonas aeruginosa 9.61 % while E.coli ,
Proteus mirabilis and Streptococcus pyogenes were 7.69% for each of them , Candida
albicans 5.76% , Klebsiella pneumonia 3.84% ,Nocardia spp 3.84% , Pasteurella multucida
3.84% ,Mannheimia haemolytica
In order to determine Candida Species isolates that associated with urogenital infection in women,174 vaginal swabs and 66 urine samples were collected from pregnant and non-pregnant women suffering from urogenital tract infection whom visted teaching Al-way hospital during the peroid from January to October 2011. The results showed that 32 out of 174 vaginal swabs were Candida Spp positive (18.3%), high percentage was reported in June and July (25%) for each one and no fungal isolates were recorded in March .The results also explained that high percentage of fungal isolates was recorded in pregnant women (68.7%) as comparing with non-pregnant women (31.2%) .Thirty –two fungal isolates were recorded which included : C.albicans(43.7%);
... Show MoreThere was a noticeable increase in cancers, congenital malformations, spontaneous
abortion and other cases among Iraqi population after both Gulf Wars I and II, the present
study aimed to the detection and determination of internal radiation pollution with Depleted
Uranium (DU), and investigate the effects of this type of radiation exposure on some
immunological parameters for the first time in Iraq. A total of 88 blood samples were
collected from 45 patients and 43 healthy persons during 2009-2010; from the outpatients of
the Institute and Hospital of Radiotherapy and Nuclear Medicine and Al-Kadhymia Teaching
Hospital in Baghdad. Results: 1. Contaminated persons with DU were 48 persons (54.5%)
from total 88 perso
Seventy four (74) samples from cattle, buffalo, goat and sheep were tested to isolate
the FMD virus, 23 isolates of the virus showed Cytopathic Effect (CPE) on the different types
of cell culture.
The CPE appears after 24 hours on fetal lamb kidney (FLK) cells, 48 hours on the fetal
bovine kidney (FBK), 30 hours on primary Fetal Bovine Thyroid cell (BTY) and 20 hours on
the primary fetal ovine thyroid cells (OTY). Solid phase ELISA test showed three serotypes
of FMD virus O, A and Asia1. One step real time RT-PCR for the diagnosis of FMDV by
using 5UTR FMDV primers and taqman probe (SA-IR-219-246F, SA-IR-315-293R and
SAmulti2-P-IR-292-269R). Samples were tested, showed 23 positive machines by using the
Applied
تم تصميم هذه التجربه لتقييم الدور الوقائي لزيت بذور الرمان (PSO) في حماية الكبد ضد السمية الناجمة عن فلوريد الصوديوم (SF) في الجرذان. قسمت عشرون من اناث الجرذان البالغات إلى أربع مجموعات متساوية وعوملت يوميا لمدة 40 يوما على النحو التالي: مجموعه السيطرة C اعطيت ماء الحنفيه ، المجموعه الثانيه T1 تلقت فلوريد الصوديوم 120 جزء في المليون في مياه الشرب ، المجموعة الثالثه T2 اعطيت كلا من فلو
... Show MoreThe study aimed at describing the histomorphological and histochemical structures of the stomach wall in domestic pigeon. Twelve healthy birds were collected from local suppliers at Baghdad province. Birds were divided into three equal groups for gross, histological and histochemical approaches. All birds were euthanized and their abdomens were dissected, then their stomachs were identified and photographed in situ. Specimens from stomach were fixed as well and then processed for histological and histochemical staining techniques using Hematoxylin-eosin, Masson Trichrome, Periodic acid Schiff (PAS) and PAS -Alcian blue (AB) (pH 2.5). Grossly the stomach of the pigeon consisted of two distinguishable chambers, tha
... Show MoreFifty locally laying hen’s eggs random samples were collected from different markets of Baghdad city in order to investigate the presence of Salmonellae Spp. in shell of those eggs. The samples were collected during the period from March 2012 to May 2012.The samples were directly transferred to the Food hygiene laboratory and analyzed immediately without further storage.The isolation and identification methods include: (pre-enrichment) culture stage by peptone water then, (Selective enrichment) culture stage by selenite broth after that culturing on sold (Selective media) which was Bismuth Sulphate agar. The biotyping by using API strip according to the API 20E miniaturized identification system for Salmonella SPP.. The isolated Salmon
... Show MoreThis research aimed at studying the effect of different concentration of hydroalcoholic leaves extract of Datura stramonium concentration against pathogenic Candida albicans isolated from clinical cases of diarrhea in cows and dogs in Baghdad province. Hydroalcoholic extract of the leaves of Datura stramonium were prepared in different concentrations for In vitro and In vivo study against Candida albicans. In vitro test includes the determination of minimum inhibitory concentration 50, 25, 12.5, 6.25, 3.12 and 1.75mg/1ml), and it was found that the minimum inhibitory concentration was 3.12 mg/1ml. Whereas the in vivo test was performed for the determination of sensitivity test of Candida al
... Show MoreThe aim of the study is to compare the histological changes that occur in the sensory neurons of dorsal root ganglion at L6 and L7 levels of the spinal nerve in different age groups in rabbits. Fifteen rabbits were divided into three groups of equal number according to their age (weaning, maturation and adult). Dorsal root ganglion of spinal nerve at L6 and L7 levels were removed and examined histologically under light microscope. Comparison were made in diameters of neurons and their numbers in different age. The results showed a significant (P<0.05) decrease in the number of sensory neurons and a significant (P<0.05) increase in their diameters with advancing age. In conclusion, the structures of sensory
... Show MoreThis study was designed to investigate the effects of the addition of different ratios of sunflower oil to the diet of Awasi ewes on the rumen fermentation and milk composition. It was conducted by using 12 Awassi ewes (2-3.5 years old) with average body weight 56.62 kg. The ewes were randomly divided into three groups. Each group had four ewes according to the age, body weight and milk yield. The ewe in each group received 1.4 kg/ dry matter for three rations consisting of barley, wheat bran, soybean meal and alfalfa hay. The first group was fed on control ration, while 2.5 and 5 % of sunflower oil were included in the rations of the second and third groups, respectively. The results showed that the treatments h
... Show MoreThis study is designed to assess the effectiveness of bovine urinary bladder submucosa on healing of cutaneous wound in Iraqi goats. A 32 (2X2) cm of full-thickness cutaneous wounds were induced in eight goats, two on each side of the lateral thoracic region. The wounds were divided into two equal groups (16 wounds/ group); treatment group included the wounds on the right side which were treated by covering the wound beds with strips of acellular sterilized bovine urinary bladder matrix. While, the wounds on the left side were left without any treatment (control group). The results were evaluated clinically (along) and histopathologically on 7, 14, 21, and 35 days post-inducing of wounds. The clinical evaluation of treated wounds showed
... Show MoreThe present study aims at using the biological techniques in a genotoxicity assessment of low doses of radiation in samples of workers in Al-Tuwaitha site due to decommissioning to radioactive contamination as a result of work. This study included 50 male blood samples, aged 35 - 63 years as well as 35 blood samples from non-smokers and non-alcoholic as control group which included 25 male and 10 female aged 25 -57 years. The endpoints used were sister chromatid exchange (SCE) and hypoxanthine guanine phosphoribosyl transferase (HPRT) mutation assay. The sister chromatid exchange in the human lymphocyte for radiation worker was significantly (P<0.05) higher than in the control group. While the results of mutat
... Show MoreThis study conducted to find out the protective role of ethanolic extract of Eruca sativa leaves against the deleterious effect of hydrogen peroxide on some aspect of male reproduction in adult rats. Forty adult male rats were randomly assigned into four equal groups as follows: control group received tap water (untreated); group T1 were received tap water containing 0.5% H2O2; group T2 were received tap water containing 0.5% H2O2 plus administration of 300 mg/kg. B.W. ethanolic extract of Eruca sativa leaves and group T3 administration ethanolic extract of Eruca sativa leaves only at the same dose of group T2.
... Show MoreThis study was performed to evaluate the reliability and efficacy of intra-peritoneal performances of chitosan powder in preventing abdominal adhesions following laparotomy. Twenty clinically healthy rabbits of both sexes, weighing 1.5-2 kg were allocated randomly into 2 equal groups; control group (G1) and treated group (G2). After surgical preparation the animals had undergone to laparotomy which was performed to create a sero-muscular incision of 4cm length in the stomach, in G1 the incision was closed by suturing stomach wound and abdominal wall, While (G2) animals were subjected to same operation but after stomach incision suturing, 1gr. of chitosan powder was sprinkled on stomach incision and peritoneal cav
... Show MoreThis study focuses on diagnosis of Clostridium difficile causing Antibiotic – Associated diarrhea and colitis by Elisa method and to detect of C. difficile Toxin A and B in stool samples by Elisa test. Two hundred forty (240) stool samples were collected from children suffering from antibiotic- associated diarrhea and Colitis cases at ages from after birth to 15 years old from Baghdad hospitals. Samples were taken during the period of first of June 2013 until the end of April 2014. In addition to that 80 samples from healthy children of the same age and sex as a control group. Clostridium difficile Toxin A and B in stool samples were detected. Results of this study in
... Show MoreA research was carried out to detect Brucella antibodies of sheep and goats in Al-Sulaimanya governorate by using modified Rose Bengal test and indirect ELISA. A total of three hundred and eleven (311) whole blood samples (160 sheep and 151 goats) were collected randomly from eight different regions in Al-Sulaimanya governorate from unvaccinated flock with different ages. A total percentages of positive result by using modified Rose Bengal test was 14.46% (20% in sheep and 8.6% in goats), while by using indirect ELISA was 27.6% (35.2% in sheep and 19.2% in goats) with significant (P<0.05) differences. It revealed that rates to modified Rose Bengal test were 14.34% in female and 10.09% in males while t
... Show MoreThe aim of this study is to determine the sensitivity of bacterial culture technique in the detection of Brucella abortus in milk samples of aborted cows. Sixty samples of milk were collected from aborted cows during a period which did not exceed two months after the abortion. All of them were positive for rose bengal test. Results showed that Brucella abortus was isolated from 7 out of 60 (11.6%) from the milk of aborted cows, while PCR test showed that 32 out of 60 (53.3%) milk sample contained Brucella abortus. The specificity of culture techniques was 10%, but its sensitivity was only 21.8%. Beside the cautions in dealing with live Brucella abortus (as culture), it is
... Show Moreيعتبر التصنيع البايولوجي للسلينيوم النانوي أمن ورخيص وصديق للبيئه ومن المواد غير السامة وعلاوة على ذلك يكون السلينيوم النانوي مستقرا بسبب احتوا ئه على الغطاء الطبيعي من المواد العضويه والتي تمنع من حدوث تراكمه مع مرور الوقت , صممت هذه الدراسه للتحقق من التوافر البايولوجي لنانوات السلينيوم المحضرة بطريقه جديده باستعمال العكبر . تم استعمال 27 جرذا بالغا وقسمت
... Show Moreأجريت هذه الدراسة لمعرفة مدى صلاحية ثلاثة أنواع من الأسماك المجمدة المستوردة للعراق هي الأسماك الكاملة (التراوت القزحي) Oncorhynchus mykiss علامة Mersin وأسماك الجري من دون رأس Pangasius hypophthalmus علامة الصياد وشرائح سمك الجري) النوع نفسه (علامة الفاخر والمشتراة من ثلاث مناطق في بغداد وهي العامرية والأعظمية والبياع أجريت الفحوص الكيميائية والنوعية لعينات الأسماك المجمدة إذ ق
... Show Moreفُحصت 100 عينة براز لبط بشكل عشوائي من خمسة مناطق مختلفة في محافظة بابل لكلا الجنسين و بأعمار مابين ستة شهور إلى سنة لتشخيص طفيلي الأبواغ الخبيئة (Cryptosporidium) وقد أظهرت النتائج إن نسبة الإصابة الكلية 8%. استعملت ثلاثة تقنيات تشخيصية مختلفة في هذه الدراسة لتشخيص أكياس بيض الطفيلي وهي الصبغة الصامدة للحامض المحورة (Modified Ziehl Neelsen) وتقنية التصبيغ بالأيودين وتقنية التطويف بالمحلول السكري المشبع. قيس
... Show MoreThe aim of this study was to investigate effect of Force 6® Poultry on lipid profile in broiler chicken in presence of IBD vaccine. Two hundred chicks at one day old were divided into four equal groups as: Group 1; 50 day old unvaccinated chicks were given (50 gm/ton) of Force 6® Poultry (curcumin) along the experimental period (35 days), chicks in group 2; were given IBD vaccine in (15-day old) (BURSINE-2), chicks in group 3 were vaccinated with IBD at (15-day old) and given Force 6® Poultry along time of experimental (with 50 gm/ton). Group 4, 50 chicks were saved as negative control. Blood samples were collected from heart for lipid profile detection and included (cho
... Show MorePigeonpox virus was isolated from severe cases of avipoxvirus infection affecting 64 pigeons in Ba'aquba of Diyala governarate. The virus grew well on chorioallantoic membrane of chick embryos of 11-12 days old, and produced typical pock lesions. Histopathological sections of infected tissue samples revealed typical pox lesions. The virus was identified as Pigeonpox virus using of specific hyper immune serum and indirect immuno-flourescent and indirect immuno-peroxidase tests. The virus agglutinated RBCs of pigeon, fowl, turkey and duck. Experimental infection in pigeons produced moderate infection as compared to the diseased birds, while in chicken the virus produced mild infection.
The present study aimed to estimate the efficiency of both a cellular bovine pericardium and bovine urinary bladder matrix sheets in the reconstruction of large ventro-lateral hernias in Iraqi bucks by using of molecular evaluation depending on real time-polymerase chain reaction technique to investigate the level of basic-fibroblast growth factor and vascular endothelial growth factor genes during the healing process and reconstruction of the abdominal defects. Under sedation and local anesthesia, (6cm X 8cm size) of ventro-lateral hernias were induced in 24 of Iraqi bucks. The animals were divided randomly into two main equal groups. In bovine pericardium-treatment group, the hernias were treated with onlay implantation of
... Show Moreاجريت الدراسة للتحري عن طفيلي Theileria spp. باستخدام الطرق التقليدية والجينية. جمعت 150 عينة دم و 50 عينة لمف من ابقار لعروق محلية ومن كلا الجنسين وذات فئات عمرية تراوحت اقل من 6 اشهر الى اكثر من سنة خلال الفصول الاربعة للعام 2018 ومن مناطق مختلفة في العراق. اظهر الفحص المجهري للمسحات الدموية المصبوغة بالكمزا نسبة اصابة كلية بثايليريا الابقار (59/150) 39.33%، وسجلت المسحات اللمفية الموجبة نسبة (17/50) 34%. احصائياً لم ت
... Show MoreThis study was designed to investigate the effect of Lactobacillus spp on the histology of cecal tonsils in broiler chicks exposed to heat stress. eighty broiler chicks were divided randomly into four groups equally and treated as follows: 1st group was administred orally with 1×108/0.1 CFU/ml of (L.B.) at one day old, 2nd group was treated as 1st group but at 21 days old, and 3rd group (control positive), all three groups were exposed to heat stress (38-40Cº) for period 10 days for three hours continuously, 4th group (control negative) was kept under natural condition. Organ samples from cecal tonsils were taken for histopathological test after
... Show MoreThe present study was carried out to investigate the antibacterial activity of alcoholic
extracts of Plantago lanceolata leafes in vitro and in vivo by inducing urinary tract infection in
rats which caused by urethra administration of S.saprophyticus isolated from human and
animals(cow and sheep)
These extracts showed significant effect (P<0.05) on the inhibition of the growth of
S.saprophyticus in vitro with the superiority of the concentration 200mg / ml of alcoholic
extract with the mean of inhibition zone diameter 30 mm against S.saprophyticus ,while zone
diameter was ( 26.5 ,21 ) mm due to the concentration 150, 100mg/ml respectively.
This study included the therapeutic role of doses 150 mg/kg . B.W. of 1.
The antimicrobial activity of Lactobacilli has been widely exploited for prevention of
food –borne pathogens e.g.: Escherichia coli being the major cause of diarrhea especially in
children, because of bacteriocin activity and the importance of herbal drugs, hence this study
was designed to evaluate the synergistic effect of plant extract and bacteriocin produced by
Lactobacillus on the growth and pathogenesis of Enteropathogenic Ecoli.
1. The Plantaricin production was induced by adding the mutagenic agent Mitomycin C.
2. Purification of Plantaricin was made by heating crude plantaricin at 80ºC for 10min and
then purified by two steps method including extraction with n-butanol followed by gel
filtration chromat
The genital organs of (89) Iraqi local cows. Collected from Al Tagi and Al- Mshahda areas of Baghdad region, were exmined for pathological pattern's Gross abnormalities of genital tract were found to the uterine affection in the study had incidence (77) included other cases in other organs addition to inactive ovary 8 cases, follicular cyst (4) cases alone : 1-Inactive ovary 27 Cases 24% Luteal cyst 3 Cases 2.67% 2-Follicular cyst 14 Cases 12.46% Ovarian aplasia 3 Cases 2.67% 3-Ovario bursal adhesion 7 Cases 6.23% Para ovarian cys 1 Case 0.89% 4-Obstruction of the oviduct 3 Cases 2.67% Poysalpinx 1 Case 0.89% 5-Acute salpingitis 5 Cases 4.45% Chronic salpingitis 7 Cases 6.23% 6-Acute endometritis 5 Cas
... Show MoreThis study was conducted on 200 samples of females Karadi sheep genitalia contains 207 fetuses . These samples were collected from slaughter house in Sulaimani reigion during the period from June 2007 to the end of May 2008. Sheep genitalia consisted of 193(96.5%) single and 7(3.5%) twin fetuses, amonge them 122 (58.92%)and 84(41.06) were in the right and left horns ,respectively. The genitalia that contain fetuses with clear external sex organs was 141, of these 74 male and 67 female fetuses different Crown Rump Length (CRL), while 66 fetus their sex could diagnosis after abdominal opened. The results showed the weight of the fetal body and testes as well as the length of scrotum were increased with an increasing of the fetal CRL. The a
... Show MoreThe study revealed that the ampullae of ductus deferens(vas deferens), seminal vesicle and bulbourethral glands are paired in number and well developed in ram and buck except the prostate gland have only disseminated part surrounding the pelvic urethra (embedded in pelvic urethral wall) without body of gland. The anatomical parameters of the ampullae in ram are generally with mean length 4.98± 0.28cm,mean width 0.63 ± 0.04cm ,mean weight 2.24 ± 0.007gms and mean volume 1.4
±0.03cm3.The seminal vesicle have mean length 3.68± 0.05cm, mean width 2.50±0.06cm ,mean weight 4.42± 0.15gms , mean volume 3.35±0.05cm3 .The bulbourethral gland have mean length 1.59± 0.36cm ,mean width 1.493±0.02cm ,mean weight 3.46±0.01gms ,mean volume