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Petroleum is one of the most important substances consumed by man at present times, a major energy source in this
century, petroleum oils can cause environmental pollution during various stages of production, transportation, refining and
use, petroleum hydrocarbons pollutions ranging from soil, ground water to marine environment, become an inevitable
problem in the modern life, current study focused on bioremediation process of hydrocarbons contaminants that remaining
in the bottom of gas cylinders and discharged to the soil. Twenty-four bacterial isolates were isolated from contaminated
soils all of them gram negative bacteria, bacterial isolates screening to investigate the ability of biodegradation of
hydrocarbons, these isolates inocula
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