This study was conducted in the botanical garden, Department of biology, College of Science/ Mustansiriyah University in from (15 February to 15 March, 2019) under the natural environmental conditions in the greenhouse in order to evaluate the effectiveness of parsley aqueous extract as a promoter for rooting. The study included the use of aqueous extract of a plant Parsley (Petroselinum crispum) extract was used in concentrations (1.25, 2.5 g / l), compare with IBA in concentration (100 mg / L) with dipping time 24 hour for all treatments. The cutting stems were included Rosmarinus officinalis, Nerium oleander, Olea europaea, Plumeria alba, Hibiscus rosa, Pelargonium graveolens, and Myrtus communis. The following measurements were taken after 30 days from the beginning of the experiment: rooting percentage, (number and length of roots), number of new (leaves and branches). Plant hormone concentrations, oxidative enzyme activity (SOD, POS) and amino acids were also analyzed for the extract that gave the highest values for all traits. The results showed that there were significant differences between treatments, and indicated that parsley extract in the concentration (2.5 g / L) was more exceeded compared with other treatments. The chemical analysis of parsley extract showed the presence of the hormones IAA, GA3, cytokinin and ABA as well as the highest activity of the antioxidant enzymes (POS and SOD), also the analysis of parsley indicated the presence of several types of amino acids, including Tryptophan, Glutamic acid, alanine, valine, Tyrosine, Phenylalanine Histadine, Ornithine, Arginine and other amino acids. In conclusion, the Parsley extract can be used as promoter rooting for stem cuttings as a new method instead of using plant growth regulators , Replace the IBA which used in the rooting of stem cuttings by some natural extracts which can stimulate the rooting process.