Background: Hybrid diabetes (or double diabetes, DD) occur when the patient which exhibits characteristics that combine type 1 diabetes (T1DM) and type 2 diabetes (T2DM). Formerly epidemiological studies found that quarter of people with T1D also had the metabolic syndrome. Subfatin, Also called cometin, it is a small (~27kDa) cytokine secreted by protein encoded by a gene called METRNL (simeler of meteorin). is much expressed in skin in the mucosal tissues and activated macrophages. Subfatin has also been described as a hormone that effected in some diseases such as metabolic diseases (including dyslipidemia), type 2 diabetes and obesity. Objectives: The current study objective is evaluating the subfatin in the blood serum of double diabetes patients to find predictive significance of diagnosis for this disease. Subjects and methods: Eighty individuals were studied , divided them into two groups . Forty patients with double diabetes represented the first group (G1), and the second group (G2), which represented the control group, consisted of (40) individuals, and the range ages of the study were (18-60)years. Whole blood was used to determine HbA1c. Samples were centrifuged, and the obtained serum was used to evaluate other biochemical markers. The technique used to determine the level of subfatin in the blood was a quantitative sandwich enzyme-linked immunosorbent assay (ELISA). Results: A significant increase shown by this study in the serum levels of subfatin in (DD) patients (n = 40) compared with control subjects (n = 40) (p value < 0.05). The ROC curves analysis for serum subfatin level when used as test for diagnosis subjects into of double diabetes cases (G1) and control group (G2), showed the AUC ( area under curve) for serum of subfatin was (1.000) have interval of confidence (95% ) and both lower and upper bound was (1.000). Conclusions: serum subfatin level could be a used as a novel biomarker of double diabetes (DD) and may contribute to the early diagnosis of diabetes.
Reactive arthritis (ReA) is an incendiary joint inflammation that occurs few days to weeks after a gastrointestinal or genitourinary infection. The etiology of the disease is not well-known. Therefore, the present study included 80 females and 25 males, divided into 51 patients with reactive arthritis and 54 healthy individuals as control group. The study involved the detection of serum levels of anti-rheumatoid factor and anti-cyclic citrullinated peptide antibodies (anti-CCP) as well as those of CRP and C3 in all subjects. In addition, EBV levels were detected by Real Time-PCR technique. The results showed significantly increased levels (P < 0.05) of CRP, C3 and anti-CCP Ab in ReA patients’ group compared to th
... Show MoreIt has been well documented that hemodialysis patients have impaired immune response, which may result in higher prevalence rates of viral infections, including CMV. Infections in these patients may be due to primary infection or, more commonly, by reactivation of latent virus or re-infection with exogenous virus, which may be introduced by blood transfusion. The aim of this study was to evaluate the frequency of CMV-IgG and IgM antibodies among hemodialysis patients. The current research included 116 hemodialysis patients (69 male and 47 female) who attended to Tikrit Teaching Hospital for the period from beginning of October/2013 to the end of April/2014. CMV-IgG was found in 102 out of 116 (87.9%), while CMV- IgM was detected in 10 ou
... Show MorePsoriasis is a common, chronic, immune-mediated skin disease with systemic pro-inflammatory activation. This study was designed to estimate the level of two cytokines, Interleukin-36 (IL-36) and Interleukin-10 (IL-10), in psoriasis female patients. The study was accomplished on 50 Iraqi patients with psoriasis who were referred to the consulting clinic at Al-Yarmouk Teaching Hospital during the period from November 2018 to March 2019. These patients were diagnosed under the supervision of dermatologists. For the purpose of comparison, the study included 30 healthy women as a healthy control group. The serum levels of cytokines were measured using the enzyme-linked immunosorbent technique (ELISA).The results of this study show
... Show MoreFifty celiac disease (CD) patients (21 males and 29 females) with an age range of 2-35 years and 25 apparently healthy controls were investigated for 10 autoantibodies (anti-tissue transglutaminase IgA antibody; ATA, anti-tissue transglutaminase IgG antibody; ATG, anti-gliadine IgA antibody; AGA, anti-gliadine IgG antibody; AGG, anti-nuclear antibody; ANA, anti-double strand DNA antibody; AdsDNA, anti-thyroid peroxidase antibody; ATP, anti-phospholipid antibody; APP, anti-myeloperoxidase antibody; AMP and anti-proteinase 3 antibody; AP3) in their sera. Six autoantibodies (ATA, ATG, AGA, AGG, AMP and AP3) showed significant variations between CD patients and controls. The first four antibodies were not detected in sera of controls, while
... Show MoreBackground: Hair loss is a common distressing disease and challenging problem for many dermatologist. Telogen effluvium is the most common hair loss disease in which nutritional deficiencies may precipitate the disease through their effect on hair structure and growth.
Study Aim : Validating role of serum ferritin level and body mass index in Chronic Telogen Effluvium and analyzing association between these factors with socioeconomic, demographic, gynecological factors and weight loss effect. Establishing a nutritional preventive advice to improve treatment successfulness and decrease the disease occurrence.
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Diabetes mellitus type 2 (T2DM) formerly called non-insulin dependent diabetes mellitus (NIDDM) or adult-onset diabetes is a common disease. Rheumatoid factor is a well-established test used in the diagnosis and follows the prognosis of rheumatoid arthritis (RA). Rheumatoid factor is sometimes found in serum of patients with other diseases including diabetes mellitus (DM), due to the presence of pro-inflammatory cytokines such as TNF- α which play an important role in chronic inflammatory and autoimmune diseases like rheumatoid arthritis (RA). The aim of the study is to investigate the associations between type 2 diabetes mellitus (T2DM) and rheumatoid arthritis (RA) in scope of rheumatoid factor (RF), hyperglycemia a
... Show MoreIn this study, 158 clinical samples were collected from hospitalized burn patients during the period from December 2012 to June 2013 in Karbala province\ Iraq. Bacterial isolates were identified using conventional biochemical tests and then identification was confirmed by using Vitek-2 compact system. Pseudomonas aeruginosa recovery was 60 isolates in this study. These isolates were analyzed for antibiotic susceptibility by the disk diffusion test (DDT) according to Kirby Bauer's method using seven clinically important antipseudomonal agents: carbapenems (Imipenem and Meropenem), pencillins (Piperacillin), cephalosporins (Ceftazidim), monobactam (Aztreonam), quinolones (Ciprofloxacin) and aminoglycosides (Gentamicin). The results of resista
... Show MoreThe present study was conducted using 16 adult male Swiss albino mice (weighing 40-50g). They were equally divided into four experimental groups. The first group was only given tap water as control during experimentation period (40 days). Group II, group III and group IV of mice were daily given a subcutaneous injection of cobalt as cobalt chloride (CoCl2) at a dose of 20mg, 40mg, and 60mg /Kg body weight respectively. The examination of the histological sections of the second experimental group of mice (low dose group) showed no particular changes in the columnar epithelial cells of the duodenum of mice. The examination of the histological sections of the third experimental group of mice (moderate dose group) showed abundant degenerativ
... Show MoreIn the present study, the effect of Zinc nanoparticles on levels of (T3 , T4 and TSH) hormones was investigated. Zinc nanoparticles were synthesized by Laser induced plasma.The Nd: YAG Nd: YAG laser with a wavelength of 1064 nm was used to generate nanomaterials of the elements (zinc) upon collision with target atoms. Plasma generated by different laser intensity is generated. After confirming the preparation of zinc nanoparticles, XRD, AFM was examined, and the effect of these substances on the thyroid gland (T3, T4, TSH) was observed for two doses of each component (1 ml / kg, 4 ml / kg) after conducting a cytotoxicity examination of the lymphocytes of the rats extracted from Rat spleen was 1.8% less toxic to zinc, and as noted The
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