The recent studies suggested the possible toxicities or genetic alterations associated with biological and medical applications of silver nanoparticles (AgNPs). The current research is directed to see if AgNPs administration can lead to some changes in expression of BRAF gene in selected body organs tissues. Fifty-six male of musmusculs (Balb/C) mice from the animal house of Al-Nahrain Centre of Biotechnology were used. These animals were divided randomly to seven groups (eight mouse in each group), one of these groups represented the control group, three groups were subjected to different doses of AgNPs (0.25, 0.5and 1 mg/kg of body weight) for one week, and the remaining three groups were subjected to three different doses of AgNPs (0.25, 0.5and 1 mg/kg of body weight) for two weeks. Liver, spleen, brain and kidneys tissue samples from each mouse in all groups (including control group) were collected, RNA was extracted and cDNAs were synthesized and then used for real time PCR and qRT-PCR Analysis. The data generated in this study indicates that the hepatic and spleen tissues expression of BRAF gene is significantly linked to AgNPs administration, also, these data showed a high significant relation between AgNPs administration and the expression of this gene in brain and kidneys tissues
Ninety eight specimens were collected from patients referring different hospitals in Baghdad in period from August to November in 2012. Specimens including (swabs from (Sputum, burn, wound, urine, ear, and eye).Sixty six isolates were identified as Pseudomonas aeruginosa. The isolates were identified according to morphological, cultural, biochemical characteristics and API 20E test. 90% of P. aeruginosa isolates produced pyocyanin pigment on King A medium in different amounts, whereas other isolates were produced other types of pigments such as (pyoverdine-yellow, pyorubin-red, and pyomelanin-black) on King B medium and also in different amounts.Quantitative assay of pyocyanin production was conducted. The results were shown that the iso
... Show MoreThe preferable design for unsaturated symmetrical spherical
double-pole piece electron lens required good focal properties, and in this present study investigate effect of air gap wide (S) on the properties of the projector lens specialized the minimum projector
focal length 1Fr) . and the properties of the axial magnetic field
\ lnlll
distribution:(the maximum
... Show MoreAbstract Background: The daily usage of maxillofacial prostheses causes them to mechanically deteriorate with time. This study was aimed to evaluate the reinforcement of VST50F maxillofacial silicone by using yttrium oxide (Y2O3) nanoparticles (NPs) to resist aging and mechanical deterioration. Materials and Method: Y2O3 NPs (30–45nm) were loaded into VST50F maxillofacial silicone in two weight percentages (1 and 1.5 wt%), which were predetermined in a pilot study as the best rates for improving tear strength with minimum increase in hardness values. A total of 120 specimens were prepared and divided into the control and experimental groups (with 1 and 1.5 wt% Y2O3 addition). Each group included 40 specimens, 10 specimens for each paramet
... Show MoreEscherichia coli infections are becoming difficult treated because of extensive resistance to antibiotic among these organisms and manufacturing extended-spectrum beta lactamases enzymes (ESBLs) make them resistant to beta-lactam antibiotics. This study aims to offer a summary of the main horizontal transmission apparatuses between E. coli as well as Staphylococcus aureus and emergence resistance to antibiotics. Fifty of the E. coli and 50 of S. aureus isolates were examined to obtain minimum inhibitory concentration (MIC) results. These isolates were then tested by conventional polymerase chain-reaction for the existence or absenc
... Show MoreVegetable sold oils (fat of the plant) were studied spectroscopy to determine its properties when it heating more than once, in this study the number of heating was 20 times and show the fat of the plant has been greatly affected by the heat. Aluminum and stainless steel were adopted for heating purposes. It turns out that the quality of the heating pot affects the spectrum specification. Changing the registered spectrum specifications for vegetable fat means a change in the characteristics of the same substance, which makes cooking use a second time with risks. Results were re-examined after six months showing significant risk of storage after heating to 300 oC the study proved that the structure of heating the vegetable oils changes an
... Show MoreOne of the serious environmental challenges that Iraq faces is climate changes and impacts of changing weather patterns and extreme global weather events. This paper addresses changes in the temporal and spatial characteristics of water levels of Razzaza Lake and response to climatic changes using archived series of Multispectral satellite images Landsat. TM, ETM+ and OLI images acquired on 1990, 2000 and of 2016. In order to extract, mapping the water surface area of the Razzaza Lake, Multispectral spectral band rationing the Normalized Difference Water Index (NDWI) technique was adopted, and the climatic elements data for the period (1990-2016) were analyzed which provide significant information of surfac
... Show MoreIn this paper, Cu2S/CdS solar cells were prepared with different thickness of CdS layer, these layers were prepared by using chemical spray pyrolysis technique. The chemical spray solution was prepared by mixing cadmium chloride CdCl2 and thiourea CS(NH2)2 of molar concentration 0.1 M/L, the CdS layer was formed after the solution was sprayed on hot Aluminum substrate at temperature 400°C. Experimentally the type of CdS film was found as n–type depending on the results of Hall Effect, the value of the Hall factor (RH) is about – 1.348 x 10–6 m3/C and the density of the majority charge carriers (N) is about 4.64x1018 cm–3. The prepared film was tested by using X-Ray
... Show MoreThe shortage of irrigation water requires specific measures. One of these measures is the application of the rationing system (a period of irrigation followed by a period of drought). This system could have an effect on the behavior and properties of irrigation canals. So, studying rationing system on the irrigation canals is important both in civil engineering and water resources engineering, especially if these channels constructed with gypsum soil. This study includes the calculation of seepage velocity and water content in each cycle (10 days wetting and 10 days of drying). The model is built for this research contains four samples, two samples for untreated soil one of them expos
Phase change materials are known to be good in use in latent heat thermal energy storage (LHTES) systems, but one of their drawbacks is the slow melting and solidification processes. So that, in this work, enhancing heat transfer of phase change material is studied experimentally for in charging and discharging processes by the addition of high thermal conductive material such as copper in the form of brushes, which were added in both PCM and air sides. The additions of brushes have been carried out with different void fractions (97%, 94% and 90%) and the effect of four different air velocities was tested. The results indicate that the minimum brush void fraction gave the maximum heat transfer in PCM and reduced the time
... Show More