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Cloning and expression of a lipase gene from Pseudomonas aeruginosa into E.coli
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Fifteen local isolates of Pseudomonas were obtained from several sources such as soil, water and some high-fat foods (Meat, olives, coconuts, etc.). The ability of isolates to produce lipase was measured by the size of clear zone on Tween 20 solid medium and by measuring the enzymatic activity and specific activity. Isolate M3 (as named in this study) was found to be the most efficient for the production of the lipase with enzymatic activity reached 56.6 U/ml and specific activity of 305.94 U/mg. This isolate was identified through genetic analysis of the 16S rRNA gene. and it was shown that the isolate M3 belongs to Pseudomonas aeruginosa with 99% similarity. The DNA of isolate M3 was extracted and lipase gene was amplified through PCR technique, then purified and cloned into E.coli DH5α cells first using pTG19-T plasmid, and expressed in E.coli Bl21 with expression vector pet-28a. The activity of lipase from transformed E.coli Bl21 was 196.6 U/ml and the specific activity 618.2 U/mg.

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Publication Date
Mon Jan 01 2024
Journal Name
2nd International Conference For Engineering Sciences And Information Technology (esit 2022): Esit2022 Conference Proceedings
Finding timewise diffusion coefficient from nonlocal integral condition in one-dimensional heat equation
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Publication Date
Sat Apr 01 2023
Journal Name
Baghdad Science Journal
Carbon Nanotubes: Synthesis via Flame Fragment Deposition (FFD) Method from Liquefied Petroleum Gas
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The current study uses the flame fragment deposition (FFD) method to synthesize carbon nanotubes (CNTs) from Iraqi liquefied petroleum gas (LPG), which is used as a carbon source. To carry out the synthesis steps, a homemade reactor was used. To eliminate amorphous impurities, the CNTs were sonicated in a 30 percent hydrogen peroxide (H2O2) solution at ambient temperature. To remove the polycyclic aromatic hydrocarbons (PAHs) generated during LPG combustion, sonication in an acetone bath is used. The produced products were investigated and compared with standard Multi-walled carbon nanotube MWCNTs (95%), Sigma, Aldrich, using X-ray diffraction (XRD), thermo gravimetric analysis (TGA), Raman spectroscopy, scanning el

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Publication Date
Wed Jan 01 2025
Journal Name
World Neurosurgery
The Ventriculoperitoneal Shunt Complication Rate in Baghdad Medical City from 2019 to 2022
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Publication Date
Sat Dec 31 2016
Journal Name
Iraqi Journal Of Chemical And Petroleum Engineering
Chromium (VI) Removal from Wastewater by Electrocoagulation Process Using Taguchi Method: Batch Experiments
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Electrocoagulation is an electrochemical method for treatment of different types of wastewater  whereby sacrificial anodes corrode to release active coagulant (usually aluminium or iron cations) into solution, while simultaneous evolution of hydrogen at the cathode allows for pollutant removal by flotation or settling. The Taguchi method was applied as an experimental design and to determine the best conditions for chromium (VI) removal from wastewater. Various parameters in a batch stirred tank by iron metal electrodes: pH, initial chromium concentration, current density, distance between electrodes and KCl concentration were investigated, and the results have been analyzed using signal-to-noise (S/N) ratio. It was found that the r

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Publication Date
Sun Jun 20 2010
Journal Name
Al-qadisiya Journal For Science
Synthesis 4-phenyl -1-alkyl-1,2,4-triazoline-5-one derived from D- fructose
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The aim of the present work is the synthesis of new carbohydrate derivatives containing 1,2,4-triazole from D-fructose . To obtain these derivatives, the diacetone fructose (1 ) was chosen as the starting material, which was obtained from the reaction of anhydrous fructose with dry acetone in presence of anhydrous ferric chloride. Oxidation of ( 1) with potassium permanganate in potassium hydroxide solution gave the acid ( 2). Esterification of the acid with dimethyl sulphate gave the methyl ester (3 ). Treatment of the methyl ester (3 ) with hydrazine hydrate gave the hydrazide (4 ), which is the desired Chiron. The hydrazide (4 ) was used for the preparation of 1,2,4-triazole-5-one (6 ) derivative. These compounds was synthesized by the i

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Publication Date
Sat Mar 30 2013
Journal Name
Iraqi Journal Of Chemical And Petroleum Engineering
Reinforcement Steel Corrosion Reduction by Using Fly Ash from South Baghdad Power Plant
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Corrosion- induced damage in reinforced concrete structure such as bridges, parking garages, and buildings, and the related cost for maintaining them in a serviceable condition, is a source of major concern for the owners of these structures.
Fly ash produced from south Baghdad power plant with different concentrations (20, 25 and 30) % by weight from the cement ratio were used as a corrosion inhibitor as a weight ratio from the cement content.
The concrete batch ratio under study was (1:1.5:3) cement, sand and gravel respectively which is used in Iraq. All the raw materials used were locally manufactured.
Concrete slabs (250x250x70) mm dimensions were casted, using Poly-wood molds. Two steel bars were embedded in the central po

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Publication Date
Sun Sep 07 2014
Journal Name
Baghdad Science Journal
Lettuce Leaves as Biosorbent Material to Remove Heavy Metal Ions from Industerial Wastewater
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The current study was designed to remove Lead, Copper and Zinc from industrial wastewater using Lettuce leaves (Lactuca sativa) within three forms (fresh, dried and powdered) under some environmental factors such as pH, temperature and contact time. Current data show that Lettuce leaves are capable of removing Lead, Copper and Zinc ions at significant capacity. Furthermore, the powder of Lettuce leaves had highest capability in removing all metal ions. The highest capacity was for Lead then Copper and finally Zinc. However, some examined factors were found to have significant impacts upon bioremoval capacity of studied ions, where best biosorption capacity was found at pH 4, at temperature 50º C and contact time of 1 hour.

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Publication Date
Sun Jan 01 2017
Journal Name
8th International Conference On Agricultural, Environment, Biology And Medical Sciences
Description new species from leafhopper belongs to genus Neoalitarus Distant, 1918 in Iraq
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Publication Date
Sun Jun 07 2009
Journal Name
Baghdad Science Journal
Limits between the Cosmological Parameters from Strong Lensing Observations for Generalized Isothermal Models
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This paper including a gravitational lens time delays study for a general family of lensing potentials, the popular singular isothermal elliptical potential (SIEP), and singular isothermal elliptical density distribution (SIED) but allows general angular structure. At first section there is an introduction for the selected observations from the gravitationally lensed systems. Then section two shows that the time delays for singular isothermal elliptical potential (SIEP) and singular isothermal elliptical density distributions (SIED) have a remarkably simple and elegant form, and that the result for Hubble constant estimations actually holds for a general family of potentials by combining the analytic results with data for the time dela

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Publication Date
Thu Jan 30 2020
Journal Name
Al-kindy College Medical Journal
Conversion Rate from Laparoscopic to open Cholecystectomy in AL-kindy Teaching Hospital, Baghdad
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Background: laparoscopic cholecystectomy (LC) is getting popularity for the treating of symptomatic gall bladder disease; conversion from laparoscopic to open cholecystectomy (OC) is also common.

Objective : To find out the prevalence of causes, risk factors of conversion from LC to OC among  patient suffering from gall bladder disease, and  to explore the most common causes of conversion from laparoscopic to open cholecystectomy.

Methods: This prospective study was conducted in the department of general surgery at Alkindy teaching hospital from first of January 2016 to the end of December 2017 .Nine hundred twenty patient were included. Patient age, gender, his

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