Fifteen local isolates of Pseudomonas were obtained from several sources such as soil, water and some high-fat foods (Meat, olives, coconuts, etc.). The ability of isolates to produce lipase was measured by the size of clear zone on Tween 20 solid medium and by measuring the enzymatic activity and specific activity. Isolate M3 (as named in this study) was found to be the most efficient for the production of the lipase with enzymatic activity reached 56.6 U/ml and specific activity of 305.94 U/mg. This isolate was identified through genetic analysis of the 16S rRNA gene. and it was shown that the isolate M3 belongs to Pseudomonas aeruginosa with 99% similarity. The DNA of isolate M3 was extracted and lipase gene was amplified through PCR technique, then purified and cloned into E.coli DH5α cells first using pTG19-T plasmid, and expressed in E.coli Bl21 with expression vector pet-28a. The activity of lipase from transformed E.coli Bl21 was 196.6 U/ml and the specific activity 618.2 U/mg.
Pure Polyaniline salt, and protonation PANI by H2SO4 were synthesized by electro-chemical oxidative polymerization of aniline with acidity of H2SO4. The solution was prepared in reaction temperature equal 291 K and the acidity of aqueous solution was 1 molarities. The prepared polyaniline was characterized by FT-IR, the result indicate that the intensity is increase with increasing of applied voltage. The dc conductivity has been measured for bulk polyaniline pure and doped in the form of compressed pellet with evaporated Ohmic Al electrodes in temperature range (303-423) K. The Eav energy of the thermal rate process of the electrical conductivity was determined. The results indicate that the dc conductivity of doped samples are two or t
... Show MoreThis research raised the strategic selection factors and dimensions of the quality of higher education and what the nature of their relationship and has been collecting a sample search from the technological University of president scientific departments and administrative and scientific associates and chiefs of branches This research aims at studying factors affecting the strategic selection effects these factors in the quality of higher education and the combination of these factors has been identified as a group of selected dimensions of quality of higher education and study link relationships and affecting factors and strategic selection of (risk, previous strategies, resources, time, considerations and internal trends) and d
... Show MoreBackground: Aqueous extracts of black tea exhibited antimicrobial activity against wide range of bacteria.
Aim of study: In this investigation the antimicrobial ly active components of this extract were identified and characterized. The minimal inhibitory concentration (MIC) for each one was determined.
Subject & Methods: the active components of the aqueous extract were identified and characterized. The minimal inhibitory concentration (MIC) for each was determined. Tannic acid, theophylline, caffeine and theobromine were isolated by thin layer chromatograhy (TLC) and purified by silica gel column. MIC was assessed by using agar dilution method.
Results: Broad spectrum activity of three components excluding theobromine agai
Background: Several biological factors such as bacterial infections and immunological status are implicated in predisposing individuals to bladder cancer. Bacterial infection of urinary tract has been related to increase the risk of bladder cancer.
Patients and Methods: Resected tumors of a total of 73 patients were obtained under sterile surgical conditions. Biopsy processing samples and culture procedures of biopsy samples were mentioned in the text.
Results: Bacterial growth was observed in 48 biopsy tissues of those patients represent (65.8%) while, 25(34.2%) yielded no growth (negative results). It is obvious that E. coli is the most predominant organisms followed by K. pneumoniae and Ps. Aer
As the child is growing up, he goes through different phases which will be accompanied by physical and psychological changes. These changes set the basis for processes of designing and making children's clothes which in turn give its required benefits and meet the physiological, psychological and community needs. That will help provide the child with healthy physical and psychological growth.
The aim of this research is to recognize the decoration of clothes by colors and drawings and its role in clothes' richness and children's education. The research limits are objective, The limits are for female (3-5) years old. The research was done in teaching kindergarten in the college of education for women in 2016. The researchers found many
The topological indices are functions on the graph that do not depend on the labeling of their vertices. They are used by chemists for studying the properties of chemical compounds. Let be a simple connected graph. The Hyper-Zagreb index of the graph , is defined as ,where and are the degrees of vertex and , respectively. In this paper, we study the Hyper-Zagreb index and give upper and lower bounds for .
The importance of this research in studying the actor life existence on the stage through the performance art;where as all the literary theatrical dimensions didn't ignore the importance of the actor's performance.
So, it is shed a light on the nature of the scenery in the actor's performance and what its representations in the temporary directions ……..
The research set off from the imitation as a human instinct to the performance of the actor in post-modernity theater; so the researcher found that there is a question must be answered which is :Is the actor live a regression state in his theatrical performance ,or that the performance of the actor connect with collective conscience of human being which is repeated in certain
Let M be a weak Nobusawa -ring and γ be a non-zero element of Γ. In this paper, we introduce concept of k-reverse derivation, Jordan k-reverse derivation, generalized k-reverse derivation, and Jordan generalized k-reverse derivation of Γ-ring, and γ-homomorphism, anti-γ-homomorphism of M. Also, we give some commutattivity conditions on γ-prime Γ-ring and γ-semiprime Γ-ring .
The study examined the assessment of raw water and drinking water projects of Diyala Governorate for the year 2017, amounting to (24) projects, The average per capita supply of potable water (0.396 m3 / day/person), which is less than the global standard for the average per capita of drinking water, and constitute water rumors within the network of water transport in the province (3%), and the water of raw and drinking value within the limits allowed to be used by Iraq and the global indicators of {Total acidity, alkaline, acidic function, chlorides, magnesium, Electrical conductivity, total soluble salts, sodium, potassium, sulfates, turbidity other than (raw water)}. While the index of calcium only a value higher than the limits
... Show MoreThe effects of reinforcing polymers with glass and graphite particles on enhancing their flexural properties are investigated. Five composites were fabricated using the same polymer matrix material with different volume fractions of reinforcement particles. They comprise glass particles and graphite particles each having volume fractions of 20% and 30% as well as a hybrid composite having 10% glass and 10% graphite. Three-point bending tests using a Universal Testing Machine were carried out on specimens of the above mentioned composites, as well as specimens of the polymer matrix material to determine their flexural properties. The experimental test results indicate that the flexural stiffness of all the composites wer
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