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Hematological analysis of bromelain conjugated on silver nanoparticles in Balb/c mice

The wound healing process is incredibly intricate, consisting of a series of cellular activities. Although, this complex process has the potential to degenerate and result in chronic wound problems that are resistant to biological healing mechanisms. Nanoparticles can help to reduce inflammation, promote tissue regeneration, and accelerate wound healing. The proteolytic enzymes are believed to break down proteins and other molecules that can cause inflammation and impede the healing process. Wound was created in vivo using adult mice, and by taking blood samples the hematological parameters were evaluated to detected the effects of bromelain, silver nanoparticles and Br-AgNPs. The results shows an increased in white blood cells WBC, RBC, MCV and MCH while platelets were decreased.Overall, the use of proteolytic enzyme loaded on silver nanoparticles has been found to be beneficial for wound healing and may provide an effective alternative to traditional treatments.

Publication Date
Mon Oct 16 2023
Journal Name
Nano And Medical Materials
Preparation and analysis of silver Nanoparticles (Ag Nps) by plant extract techniques of green tea and study optical and structural properties

Aqueous root extract has been used to examine the green production of silver nanoparticles (AgNPs) by reducing the Ag+ ions in a silver nitrate solution. UV-Vis spectroscopy, X-ray diffraction, field emission scanning electron microscopy, and Fourier transform infrared spectroscopy (FTIR) were used to analyze the produced AgNPs. The AgNPs that were created had a maximum absorbance at 416 nm, were spherical in form, polydispersed in nature, and were 685 nm in size.The AgNPs demonstrated antibacterial efficacy against Escherichia coli and Staphylococcus. The dengue vector Aedes aegypti's second instar larvae were very susceptible to the AgNPs' powerful larvicidal action.

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Publication Date
Tue Jul 28 2020
Journal Name
Iraqi Journal Of Science
Toxicokinetics of Titanium Dioxide (Tio2) Nanoparticles After Intraperitoneal Injection in Male Mice

     In the present study, male albino mice were used to estimate the effects of titanium dioxide nanoparticles (TiO2) suspension used in two doses (150, 600 mg/kg) through intraperitoneal route. The results revealed a significant difference (p≤0.05) among the control and experimental groups in all haematological parameters, including a significant increase in White Blood Cell (W.B.C) count, Mean Cell Volume (MCV), Mean Cell Haemoglobin Concentration (MCHC), and Mean Cell Haemoglobin (MCH). Also, the results showed a significant decrease in Red Blood Cell (R.B.C.) count and Haemoglobin (Hb). Biochemical tests included AST and ALT and showed a significant elevation in all exposed groups, while ALP was

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Publication Date
Sun Sep 22 2019
Journal Name
Baghdad Science Journal
Comparative Antimicrobial Activity of Silver Nanoparticles Synthesized by Corynebacterium glutamicum and Plant Extracts

           Biosynthesis of nanoparticles has received considerable attention due to the growing need to develop environmentally benign nanoparticle synthesis processes that do not use toxic chemicals. Therefore, biosynthetic methods employing both biological agents such as bacteria and fungus or plant extracts have emerged as a simple and a viable alternative to chemical synthetic and physical method .It is well known that many microbes produce an organic material either intracellular or extracellular which is playing important role in the remediation of toxic metals through reduction of metal ions and acting as interesting Nano factories. As a result, in the present study Ag NPs were syn

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Publication Date
Fri Jun 24 2022
Journal Name
Iraqi Journal Of Science
Formation Silver Nanoparticles of Different Size Using Different Reductants with AgNO3 Solution

In this study, silver nanoparticles (AgNPs) are synthesized using different chemical routes to obtain different sizes and shapes of nanoparticles by colloid chemistry with using stabilizing and reducing agent, which make them interesting for variety of physical applications. The morphology and structure of the synthesized AgNPs were characterized by UV-VIS spectra, Scanning Electron Microscopy (SEM) and Zeta potential to demonstrate that different sizes and shapes can by synthesized by different reductants in the presence of various stabilizing agents.

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Publication Date
Sun Aug 01 2021
Journal Name
Nano Biomedicine And Engineering
In-vivo and In-vitro Anti-Acinetobacter Baumannii Activity of Citrate-Capped Silver Nanoparticles

Silver nanoparticles (AgNPs) are of potential interest because of their effective antibacterial and antiviral activities. Capping agents are used for exhibiting a better antibacterial activity than uncapped Ag NPs. There are very few reports that have shown the usage of AgNPs for in-vivo antibacterial therapy. Citrate-capped silver nanoparticles were synthesized chemically by citrate reduction method; the size of Cit-AgNPs was determined by an atomic force microscope (AFM) and was between 15-90 nm. Acinetobacter baumannii (A. baumannii) isolates were the only sensitive species to Cit-AgNPs. MICs and MBC of Cit-AgNPs were determined by using A. baumannii. The results showed an additive effect of Cit-AgNPs. Four mice groups were infected with

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Publication Date
Sun Mar 05 2017
Journal Name
Baghdad Science Journal
Detecting the antibacterial activity of green synthesized silver (Ag) nanoparticles functionalized with ampicillin (Amp)

In the current study, synthesis and characterization of silver nanoparticles (AgNPs) before and after functionalization with ampicillin antibiotic and their application as anti-pathogenic agents towards bacteria were investigated. AgNPs were synthesized by a green method from AgNO3 solution with glucose subjected to microwave radiation. Characterization of the nanoparticles was conducted using UV-Vis spectroscopy, scanning electron microscopy (SEM), zeta potential determination and Fourier transform infrared (FTIR) spectroscopy. From SEM analysis, the typical silver nanoparticle particle size was found to be 30 nm and Zeta potential measurements gave information about particle stability. Analysis of FTIR patterns and UV-VIS spectroscopy con

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Publication Date
Tue Sep 29 2020
Journal Name
Iraqi Journal Of Science
Scavenging of Free Radicals Generated in Biological Tissues Exposed to Ionizing Radiation Using Silver Nanoparticles

Humans are exposed to nuclear radiations every day, and these radiations are both natural and artificial. When the body tissues are exposed to nuclear radiation, free radicals are formed, which are responsible for cancer development. In this research, silver nanoparticles were synthesized by electrical explosion wire method. Nanoparticles were added to deionized water that contained free radicals before and after exposure to gamma rays. The obtained results indicate that the silver nanoparticles have antioxidant potential through possessing free radical scavenging activity, as they can donate electron to free radicals and become neutralize. Then, these nanoparticles were injected to mice before and after their irradiation with gamma ray.

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Publication Date
Tue May 30 2023
Journal Name
Iraqi Journal Of Science
Antibacterial Activity of Silver nanoparticles Against Pathogenic Bacterial Isolates from Diabetic Foot Patients

     Diabetic foot is a catastrophic complication of diabetes. This study included isolation and identification of three types of bacteria that cause diabetic foot ulcers,  fifty-five isolates of Staphylococcus aureus, thirty-five isolates of Acinetobacter baumannii, and thirty isolates of Serratia marcescens. These isolates were obtained from diabetic foot patients at different private clinics in and around Baghdad and Medical City Hospital. The proportion of male patients was greater than females, and it was noted that the age group (51-68 years) was more ages affected by diabetic foot. These isolates showed high resistance to most of the antibiotics used, Staphylococcus aureus was resistant to

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Publication Date
Sun Mar 04 2018
Journal Name
Iraqi Journal Of Science
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Publication Date
Wed Oct 16 2019
Journal Name
Journal Of Research In Medical And Dental Science
Evaluation of the Antibacterial Efficacy of Silver Nanoparticles as an Irrigant against Enterococcus faecalis In vitro Study

Background: Successful root canal therapy depends on thorough chemo mechanical debridement of pulpal tissue, dentin debris and infective microorganisms. Objective: This study aimed to investigate the antibacterial effect of silver nanoparticles, sodium hypochlorite and chlorhexidine in reducing the bacterial infection of the root canals. Materials and Methods: The root canals of 55 single-rooted teeth were cleaned, shaped, and sterilized. All the teeth samples were inoculated with Enterococcus faecalis and incubated at 37°C for 2 weeks. Then, the teeth were divided into four groups. Group I (n=15): 100 ppm silver nanoparticles, Group II (n=15): 2.5 sodium hypochlorite, Group III (n=15): 2% chlorhexidine, IV (n=10): Normal saline as a contr

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