This study aimed to study the inhibition activity of purified bacteriocin produced from the local isolation Lactococcuslactis ssp. lactis against pathogenic bacteria species isolated from clinical samples in some hospitals Baghdad city. Screening of L. lactis ssp. Lactis and isolated from the intestines fish and raw milk was performed in well diffusion method. The results showed that L. lactis ssp. lactis (Lc4) was the most efficient isolate in producing the bacteriocin as well observed inhibitory activity the increased that companied with the concentration, the concentration of the twice filtrate was better in obtaining higher inhibition diameters compared to the one-fold concentration. The concentrated bacteriocin was purified using the gel filtration column and Sephacryl S-200. The results showed the high inhibitory activity of the purified bacteriocin after the purification against the positive and negative bacteria of the Gram stain under study compared to the one-fold concentration and two-fold before purification , The diameters of the inhibition zones after gel-filtering of the purified bacteriocin reached S. aureus, S. epidermidis, P. aeruginosa, E. coli, E. clocae and S. marcescens (23, 25, 26, 20, 22 and 28) Mm respectively. The carbohydrate content of purified bacteriocin from L. lactis ssp. lactis (Lc4) isolate was 6.02% with a molecular weight of 6310 Dalton. The results showed that purified bacteriocin retained its inhibitory activity at pH 2-10 and showed the highest inhibition at pH 4-6 and lost at pH 12. The purified bacteriocin was characterized by thermal stability. It retained its effectiveness when exposed to 40, 60, 80, 100°C for 30, 15, 5 minutes and 120°C for 15.5 minutes and lost 50% of its effectiveness when exposed to 120°C for 30 minutes. Results The purified bacteriocin was effectively retained when treated with enzyme pepsin and trypsin of 37°C for one hour and at pH 7.
In this research, a selection of some mineral water was selected on the basis of being the most marketed by the owners of shops in Najaf province, with six types, where daily samples of this water were taken by 50 ml for two months from (1/11/2018 -1/1/2019). The following ions concentrations were measured (Br-, Cl-, F-, NO3-, SO42-, Na+, K+, Ca2+, Mg2+), pH and the electrical conductivity were measured and the results were compared with the allowable rates according to the international organizations. It was noted that they conform to international and Iraqi standards.
The electrospun nanofibers membranes have gained considerable interest in water filtration applications. In this work, the fabrication and characterization of the electrospun polyacrylonitrile-based nonwoven nanofibers membrane are reported. Then, the membrane's performance and antifouling properties were evaluated in removing emulsified oil using a cross flow filtration system. The membranes were fabricated with different polyacrylonitrile (PAN) concentrations (8, 11, and 14 wt. %) in N, N-Dimethylformamide (DMF) solvent resulted in various average fiber sizes, porosity, contact angle, permeability, oil rejection, and antifouling properties. Analyses of surface morphology of the fabricated membranes before and after oil removal revealed
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The characteristics of sulfur nanoparticles were studied by using atomic force microscope (AFM) analysis. The atomic force microscope (AFM) measurements showed that the average size of sulfur nanoparticles synthesized using thiosulfate sodium solution through the extract of cucurbita pepo extra was 93.62 nm. Protecting galvanized steel from corrosion in salt media was achieved by using sulfur nanoparticles in different temperatures. The obtained data of thermodynamic in the presence of sulfur nanoparticles referred to high value as compares to counterpart in the absence of sulfur nanoparticles, the high inhibition efficiency (%IE) and corrosion resistance were at high temperature, the corrosion rate or weig
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The research deals with the subject of «ways to face rumors in the site of social networking Facebook», a research based on a doctoral thesis, as the research dealt with rumors in the most dangerous type of new media, which sites of social communication with the global community as spreading rumors of all kinds and afflicts everyone who has eyes on him Of the audience of these sites, and aims to identify the rumors on Facebook because it is one of the most popular sites in the world and the strategy of combating the rumors published by Facebook users in their personal pages and groups that organize them, as well as identify methods and methods that can be During which the face of the rumors in the said site and eliminate them t
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سارت اليهودية على نهج التوحيد بعدما بعث الله تعالى موسى (عليه السلام) ليبلغهم رسالات ربهم، ليكونوا على هدى وصراط قويم، غير أن القوم لم يستقيموا على ذلك الصراط" فبدلوا وأولوا وتمردوا على موسى (عليه السلام) وصوروا الرب حسب أهواء أنفسهم لأنهم جبلوا على حب المادة والإيمان بالمشهود، فصوروا الرب بصور عدة بما يتماشى مع ما الفت عليهم نفوسهم .
الحمد لله رب العالمين والصلاة والسلام على نبينا محمد صلى الله عليه وآله وصحبه وسلم اجميعن.
وبعد...
فان من أعظم مظاهر اعجازه البياني ذلك التشابه العجيب بين كثير من آياته ، وقد اكتفيت في هذا البحث عن موضوعين ، وقد بينت التشابه بوصف اللفظي حتى لا ينصرف الذهن الى المتشابه المقابل للمحكم ، وقد اظهر البحث سمة الترتيب ، ولاسيما الترتيب داخل الجملة ، ويتضح ذلك في موضوع التقديم والتأخير بين الجمل في ا
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يرجع تأريخ ظهور الفساد إلى بداية الخلق والخليقة، إذ اهتم الأديان السماوية بها كثيراً; وذلك لتوعية الناس إلى أن جَلل المصاب تكمن وراء هذه الظاهرة.
والقرآن الكريم اهتم بهذا الجانب اهتماماً بالغاً، فقد ذكر تأثيرات الفساد على المجتمعات عموماً، وعلى الأفراد خصوصاً، ولم تأتِ آية كريمة تتحدث عن آثا
... Show Moreالأسطورة ذلك العالم الغريب المجهول الذي يرويه الناس بعضهم يؤمنون به وآخر يبقى بعيد الصلة عنه ولكن لا يمكن أن يشكك به وبما يعتقد به البعض الآخر، تبقى متوارثة من جيل لجيل حتى تتذكر جزئياً أو كلياً وحسب ثقافة الكائن البشري. وفي معطيات كثيرة نجد الإنسان لا يتوانى أن يوعز تلك الأشياء إلى مجهول مسيطر ومهيمن، يستطيع أن يكون متحرراً من كثير من الالتزامات والإشكالات التي يعاني منها الإنسان، وهو في هذا إنما ينتمي أو ي
... Show MoreABSTRACT: Pathogenic bacteria responsible for the causation of many common diseases have been identified on currency notes. The present investigation was carried out on one hundred currency notes of all the denominations (50, 100, 250, 500 and 1000RY), obtained from different occupational mainly bus drivers, hawker street, vegetable vendor, restaurants and butchers and fish seller groups in Taiz city,Yemen. Identification and characterization revealed active participation of the following species of organisms in the ascending order of percentage as E. coli(50.28 %),Staphylococci aureus(14.04 %), Klebsiellaspp(4.39 %),proteus(4.39 %), salmonella(1.25 %), shigella(0.72 %), Coagulase negative staphylococcus(0.60 %), pseudomonas(0.50 %),
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