This study aimed to study the inhibition activity of purified bacteriocin produced from the local isolation Lactococcuslactis ssp. lactis against pathogenic bacteria species isolated from clinical samples in some hospitals Baghdad city. Screening of L. lactis ssp. Lactis and isolated from the intestines fish and raw milk was performed in well diffusion method. The results showed that L. lactis ssp. lactis (Lc4) was the most efficient isolate in producing the bacteriocin as well observed inhibitory activity the increased that companied with the concentration, the concentration of the twice filtrate was better in obtaining higher inhibition diameters compared to the one-fold concentration. The concentrated bacteriocin was purified using the gel filtration column and Sephacryl S-200. The results showed the high inhibitory activity of the purified bacteriocin after the purification against the positive and negative bacteria of the Gram stain under study compared to the one-fold concentration and two-fold before purification , The diameters of the inhibition zones after gel-filtering of the purified bacteriocin reached S. aureus, S. epidermidis, P. aeruginosa, E. coli, E. clocae and S. marcescens (23, 25, 26, 20, 22 and 28) Mm respectively. The carbohydrate content of purified bacteriocin from L. lactis ssp. lactis (Lc4) isolate was 6.02% with a molecular weight of 6310 Dalton. The results showed that purified bacteriocin retained its inhibitory activity at pH 2-10 and showed the highest inhibition at pH 4-6 and lost at pH 12. The purified bacteriocin was characterized by thermal stability. It retained its effectiveness when exposed to 40, 60, 80, 100°C for 30, 15, 5 minutes and 120°C for 15.5 minutes and lost 50% of its effectiveness when exposed to 120°C for 30 minutes. Results The purified bacteriocin was effectively retained when treated with enzyme pepsin and trypsin of 37°C for one hour and at pH 7.
הקדמה
הריאליזם הספרותי במשמעו הכללי מצייג אשר כל מה מציינת בה הספרות מציורים מדוייקים לסביבה ולכן אדם. ויש להתחשב בפרטים המשותפים לחיים היומיומית.
האסכולה הריאליזטית מצטיינת בזיאנרים אין זוכים בם איזו אסכולה ספרותית אחרת. האסכולה הזאת הופיעה בספרות העברית החדשה כתוצאה להשפעתה של הספרות העברית בס
... Show MoreThis paper deals with defining Burr-XII, and how to obtain its p.d.f., and CDF, since this distribution is one of failure distribution which is compound distribution from two failure models which are Gamma model and weibull model. Some equipment may have many important parts and the probability distributions representing which may be of different types, so found that Burr by its different compound formulas is the best model to be studied, and estimated its parameter to compute the mean time to failure rate. Here Burr-XII rather than other models is consider because it is used to model a wide variety of phenomena including crop prices, household income, option market price distributions, risk and travel time. It has two shape-parame
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تتركز مشكلة البحث في ضعف كثير من المربين معلمين كانوا أو مدرسين في التعامل مع أخطاء المتعلمين في ضوء السنة النبوية مما يؤدي إلى تعلم غير منتظم، ومن أهم أسباب هذه المشكلة قلة الإطلاع على الأساليب العملية التي ينبغي أن يتعامل بها المربي مع أخطاء المتعلمين، ولا سيما الأساليب المستمدة من القرآن الكريم والسنة النبوية، وهذا ما لمسه الباحث واقعاً من خلال ممارسته لعملتي التعليم والتدريس لمدة خمس عشرة سنة، ومن خ
... Show Moreان حـق التنميـة والتطـور مـن احلقـوق التـي طالبـت هبـا املجتمعـات الدوليـة كافـة السـيام بعـد احلــرب العامليــة الثانيــة، ولكــن العقوبــات االقتصاديــة التــي فرضــت عــى العــراق مل تراعــي حقــه يف التنميــة فبعــد ان كان العــراق يتجــه نحــو التقــدم والتطــور يف منطقــة الــرق االوســط عــاد إىل التدهـور والضعـف بسـبب فـرض العقوبـات االقتصاديـة عليـه مـن قبـل املجتمـع الـدويل ، ونظـرا المهي
... Show MoreGod Almighty sent down the Noble Qur’an to guide people in all areas of their lives, including the field of functional business.
Assyrian merchants established in the second millennium BC Assyrian commercial settlements in Anatolia, and these settlements had a great cultural value
Chekhov is well known and perceived in Arab countries. His stories and plays are very popular. They translated it into Arabic by different translators from different languages of the world Many of his stories require new translation solutions to achieve partial, if not complete, equivalence. Chekhov's works are a very difficult subject to analyze and interpret, which is explained by the fact that Chekhov's collections are constantly republished in foreign languages. It is impossible to preserve in translation all the elements of the original text containing historical and national details but, of course, the reader should have the impression that they represent the historical and national situation. When translating, it makes sense to prese
... Show Moreالحمد لله خالق الأكوان، وبارئ الإنسان، عظيم الشأن والإحسان، والصلاة والسلام على خاتم الأنبياء والمرسلين الصادق الأمين محمد وعلى آله الطيبين الطاهرين وعلى من سار على هديه بإحسان إلى يوم الدين.
أما بعد ...
فالقرآن الكريم هو ذروة الفصاحة والإعجاز والسلامة اللغوية يمثل بقراءاتهِ القرآنية المتعددة: المتواترة والشاذة والآحاد أصلا أصيلا للنحو العربي يحتج به في إقامة
... Show MoreIslamic civilization derived its religious law from Quran and sunnah which took care of human as a benefit person in life and how it treat with it , and because the civilization takes care of human , it would take also in his health , manners ,body and this is the aim of Islamic civilization ,all religious emphasize on Islamic laws and on helping poor people or weakness one.
كانت المجالس وما ا زلت تعقد فػي البيػوت او الػدواويف الرسػمية ينػاقش فيهػا المجتمعػوف امػوره الخاصػػة والعامػػة وقػػد اخػػذت هػػذ المجػػالس عمػػ عاتقهػػا نشػػر عمػو العممػػا والقصائد الشعرية والاخبار السياسية والفكاهية وكاف يحضرها مختمؼ ش ا رئح المجتمع ساعدت هػذ المجػالس عمػ حفػظ التػ ا رث العربػي الاسػلامي مػف مثُػؿ وقػي خمقيػة لكػف بسبب الفػتف المذهبيػة التػي سػادت المجتمػع فػي القػرف الخػامس الهجػري
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