This study aimed to study the inhibition activity of purified bacteriocin produced from the local isolation Lactococcuslactis ssp. lactis against pathogenic bacteria species isolated from clinical samples in some hospitals Baghdad city. Screening of L. lactis ssp. Lactis and isolated from the intestines fish and raw milk was performed in well diffusion method. The results showed that L. lactis ssp. lactis (Lc4) was the most efficient isolate in producing the bacteriocin as well observed inhibitory activity the increased that companied with the concentration, the concentration of the twice filtrate was better in obtaining higher inhibition diameters compared to the one-fold concentration. The concentrated bacteriocin was purified using the gel filtration column and Sephacryl S-200. The results showed the high inhibitory activity of the purified bacteriocin after the purification against the positive and negative bacteria of the Gram stain under study compared to the one-fold concentration and two-fold before purification , The diameters of the inhibition zones after gel-filtering of the purified bacteriocin reached S. aureus, S. epidermidis, P. aeruginosa, E. coli, E. clocae and S. marcescens (23, 25, 26, 20, 22 and 28) Mm respectively. The carbohydrate content of purified bacteriocin from L. lactis ssp. lactis (Lc4) isolate was 6.02% with a molecular weight of 6310 Dalton. The results showed that purified bacteriocin retained its inhibitory activity at pH 2-10 and showed the highest inhibition at pH 4-6 and lost at pH 12. The purified bacteriocin was characterized by thermal stability. It retained its effectiveness when exposed to 40, 60, 80, 100°C for 30, 15, 5 minutes and 120°C for 15.5 minutes and lost 50% of its effectiveness when exposed to 120°C for 30 minutes. Results The purified bacteriocin was effectively retained when treated with enzyme pepsin and trypsin of 37°C for one hour and at pH 7.
يتناول هذا البحث التحول الجذري في الفن الحديث من الكيان المادي (كاللوحة) إلى "الفكرة" كمحور للعمل الفني، مركزًا على الفن المفاهيمي وإشكالية "اللامُقتنى". تم في المبحث الأول تحليل العلاقة المعقدة بين الفن والسوق، وكيف سعى الفن المفاهيمي إلى تفكيك منطق التسلّع من خلال أعمال يصعب اقتناؤها، مثل عمل "الموزة" لكاتيلان، الذي أثار جدلاً حول جدوى التسعير في الفن. أما المبحث الثاني فقد ركّز على فن التركيب كوسيط فني ي
... Show Moreتم تحضرالكاربون المنشط النانوي (NAC) من مخلفات النباتات باستخدام فرن الميكروويف وذلك بخلط نسب مختلفة من قشور الرز مع مخلفات البولي إثلين واطىء الكثافة (LDPE) وكاربونات البوتاسيوم كعامل تنشيط كيميائي في عملية الكربنة، تم تنفيذ كل من عمليتي الكربنة والتنشيط عن طريق تشعيع الموجات الدقيقة بقوة 600 واط لمدة 15 دقيقة، وبنسب وزنية مختلفة لكل من قشور الرز والمخلفات البوليمر. تم قياس المساحة السطحية الخارجية لعينات
... Show Moreفي هذا البحث حضر الكاربون المنشط ذو الحجم الحبيبي في المدى المايكروي والمدى النانوي من قشور الجوز واجريت عملية الكربنة باستخدام فرن المايكرويف ومن ثم استعمل محلول (%50) من حامض الفسفوريك مع المادة المكربنة وبأزمان مختلفة (2,4,8,16,24) ساعة لتحديد الزمن الأفضل لعملية التنشيط في هذا البحث ، وبعد ذلك اضيفت نسب وزنية مختلفة من مخلفات بولي بروبلين عند زمن (8) ساعة وكانت النسب 0.05):,1 0.1:,1 0.15:0.2 ,1: (1:0.25 ,1 للمضاف البوليمري ومس
... Show Moreفسيفساء قبة الصخرة دراسة تحليلية
Bidentate Schiff base ligand 3-(3,4-Dihydroxy-phenyl)-2-[(4-dimethylamino-benzylidene)-amino]-2-methyl-propionic acid was prepared and characterized by spectroscopic techniques studies and elemental analysis. The Cd(II), Ni(II), Cu(II), Co(II), Cr(III),and Fe(III) of mixed-ligand complexes were structural explicate through Moler conductance , [FT-IR, UV-Vis & AAS], chloride contents, , and magnetic susceptibility measurements. Octahedral geometries have been suggested for all complexes. The Schiff base and its complexes were tested against various bacterial species, two of {gram(G+) and gram(G-)} were shown weak to good activity against all bacteria.
السياسة الخارجية الايرانية تجاه الولايات المتحدة (1979-2008)
Summery of the Study: The Israeli interest in Iraq was not the result of the 2003 US war, which ended with its occupation, but Iraq was still at the top of Israeli concerns, as it was due to its interest and desire to occupy the Zionist movement for a number of reasons, most importantly its religious position in the Jews and control It is a sacred religious duty, so Israel has employed all its organs, institutions, relations and espionage networks in order to penetrate it and perpetuate its existence, and succeeded in achieving its foreign policy objectives at a time when the area was open to it without opposition or competition thanks to its strategic alliance with the states Of the United States of America, which has been able to penet
... Show MoreThis article aims to explore the importance of estimating the a semiparametric regression function ,where we suggest a new estimator beside the other combined estimators and then we make a comparison among them by using simulation technique . Through the simulation results we find that the suggest estimator is the best with the first and second models ,wherealse for the third model we find Burman and Chaudhuri (B&C) is best.