This study aimed to study the inhibition activity of purified bacteriocin produced from the local isolation Lactococcuslactis ssp. lactis against pathogenic bacteria species isolated from clinical samples in some hospitals Baghdad city. Screening of L. lactis ssp. Lactis and isolated from the intestines fish and raw milk was performed in well diffusion method. The results showed that L. lactis ssp. lactis (Lc4) was the most efficient isolate in producing the bacteriocin as well observed inhibitory activity the increased that companied with the concentration, the concentration of the twice filtrate was better in obtaining higher inhibition diameters compared to the one-fold concentration. The concentrated bacteriocin was purified using the gel filtration column and Sephacryl S-200. The results showed the high inhibitory activity of the purified bacteriocin after the purification against the positive and negative bacteria of the Gram stain under study compared to the one-fold concentration and two-fold before purification , The diameters of the inhibition zones after gel-filtering of the purified bacteriocin reached S. aureus, S. epidermidis, P. aeruginosa, E. coli, E. clocae and S. marcescens (23, 25, 26, 20, 22 and 28) Mm respectively. The carbohydrate content of purified bacteriocin from L. lactis ssp. lactis (Lc4) isolate was 6.02% with a molecular weight of 6310 Dalton. The results showed that purified bacteriocin retained its inhibitory activity at pH 2-10 and showed the highest inhibition at pH 4-6 and lost at pH 12. The purified bacteriocin was characterized by thermal stability. It retained its effectiveness when exposed to 40, 60, 80, 100°C for 30, 15, 5 minutes and 120°C for 15.5 minutes and lost 50% of its effectiveness when exposed to 120°C for 30 minutes. Results The purified bacteriocin was effectively retained when treated with enzyme pepsin and trypsin of 37°C for one hour and at pH 7.
To determine the expression of key epithelial–mesenchymal transition (EMT) markers in gingival tissue samples collected from patients with periodontitis.
Epithelial–mesenchymal transition is a process responsible for shifting epithelial‐phenotype to mesenchymal‐phenotype leading to loss of epithelial‐barrier function. Thus, EMT could be involved as a pathogenic mechanism in periodontitis as both conditions share common promoters and signalling pathways.
Gingival tissue samples were collected fro
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The study aimed to determine of some Optimum conditions for bioremediation and removing of seven mineral elements included hexavalent chromium, nickel, cobalt, cadmium, lead, iron and copper as either alone or in group by living and heat treated cells of baker’s yeast Saccharomyces cerevisiae. The dried baker's yeast from Aldnaamaya China Company was used in this study. Biochemical tests was used to ensure yeast belonging to S. cerevisiae and then used to remove the mentioned mineral elementes under different conditions which included incubation period, pH, and temperature. It was found that the best of these conditions was 60 minutes for duration of incubation, 6 for pH, 25 ᵒC for temperature. During the study the behavior of living
... Show MoreThe research topic (The Impact of the Compulsory Reserve on the Banks Credit-an applied research on a sample of Iraqi banks), was chosen on basis of the Iraqi private Banks attempts to increase their credit activities in hope to gain more profits.This coincided with the CBI aim to back the private banking market.It is thus apparent why the CBI issued its regulation to those banks to increase their capitals up to IDB250. On the other hand,the CBI acted as an obstacle in the way of increasing the private banks of their capitals,that is by implementing the quantitive monetary tool so called the compulsory reserve.This is why we intended to overlook at this contradictional problome
... Show Moreتلخصت مقدمة البحث بأن التحليل الحركي الميكانيكي وسيلة مهمة بواستطتها يتم تشخيص وتقيم الظاهرة بشكل موضوعي وفق أسس علمية ثابتة ،بأستخدام التقنية الحديثة، وكرة اليد من بين الألعاب التي ظهرت في الآونة الأخيرة بمستوى أداء يجذب إعجاب المشاهد لهذه اللعبة، كماأن الدقة في التصويب تعد مفتاح لتحقيق الهدف من خلال وصول الكرة إلى المكان الصحيح ودخولها إلى المرمى قبلَ أن تكون فرصة للصد . و مشكلة البحث بان اللاعب يهدر
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قمنا بتحليل مراحل تطور الرواية الروسية في القرن التاسع عشر. وبحثنا في طرائق تصوير الواقع الروسي وما بعد الواقعية. ونحاول الإجابة عن الأسئلة الآتية: لماذا سُمّيَ القرن التاسع عشر بالقرن الذهبي؟ ولماذا في النصف الثاني من القرن التاسع عشر أصبحت الرواية الروسية واجهة للحركة الأدبية في روسيا؟ وتجدر الإشارة إلى أن الرواية الروسية خلال هذه الحقبة وصلت إلى أعماق النفس البشرية بطريقة غير مسبوقة، عن طريق عر
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شهدت السينما اثناء عمرها الممتد عبر نحو قرن من الزمن احداثا وتطورات سياسية مهمة، فضلا عن التطورات التقنية والفنية وكانت السينما حاضرة وشاهدة على احداث سياسية بارزة مر بها العالم قبل واثناء وبعد الحربين العالميتين الاولى والثانية وفترة الحرب الباردة وما بعده. وقد حظيت السينما اثناء النصف الاول من القرن العشرين باهتمام كبير من قبل الدول الكبرى والسياسيين فيها فضلا عن الاقبال التجاري للشرك
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