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STUDY THE INHIBITION ACTIVITY OF PURIFIED BACTERIOCIN FROM LOCAL ISOLATION Lactococcuslactis ssp. lactisagainst SOME PATHOGENIC BACTERIAL SPECIES ISOLATED FROM CLINICAL SAMPLES: دراسة الفعالية التثبيطية للبكتريوسين المنقى من العزلة المحلية Lactococcuslactis ssp. Lactis تجاه بعض البكتريا الممرضة المعزولة من عينات سريرية
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This study aimed to study the inhibition activity of purified bacteriocin produced from the local isolation Lactococcuslactis ssp. lactis against pathogenic bacteria species isolated from clinical samples in some hospitals Baghdad city. Screening of L. lactis ssp. Lactis and isolated from the intestines fish and raw milk was performed in well diffusion method. The results showed that L. lactis ssp. lactis (Lc4) was the most efficient isolate in producing the bacteriocin as well observed inhibitory activity the increased that companied with the concentration, the concentration of the twice filtrate was better in obtaining higher inhibition diameters compared to the one-fold concentration. The concentrated bacteriocin was purified using the gel filtration column and Sephacryl S-200. The results showed the high inhibitory activity of the purified bacteriocin after the purification against the positive and negative bacteria of the Gram stain under study compared to the one-fold concentration and two-fold before purification , The diameters of the inhibition zones after gel-filtering of the purified bacteriocin reached S. aureus, S. epidermidis, P. aeruginosa, E. coli, E. clocae and S. marcescens (23, 25, 26, 20, 22 and 28) Mm respectively. The carbohydrate content of purified bacteriocin from L. lactis ssp. lactis (Lc4) isolate was 6.02% with a molecular weight of 6310 Dalton. The results showed that purified bacteriocin retained its inhibitory activity at pH 2-10 and showed the highest inhibition at pH 4-6 and lost at pH 12. The purified bacteriocin was characterized by thermal stability. It retained its effectiveness when exposed to 40, 60, 80, 100°C for 30, 15, 5 minutes and 120°C for 15.5 minutes and lost 50% of its effectiveness when exposed to 120°C for 30 minutes. Results The purified bacteriocin was effectively retained when treated with enzyme pepsin and trypsin of 37°C for one hour and at pH 7.

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Publication Date
Thu Oct 01 2015
Journal Name
Journal Of Educational And Psychological Researches
Psychological contents As Deduced From Al Namel Surah, Dialogue between Suliman and Hodhod bird & Their Application In School Counseling
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The current research aimed at deducing the psychological contents of Suliman dialogue with the Hodhod and statement applications in school counseling. The researcher followed the Islamic approach in the search, which deals with the study of events, phenomena and practices through a broad understanding of Islamic principles and limitations associated with the general framework of Islam. In addition to the deductive approach is derived from a sub-rule is a general provision.
The research revealed many of the psychological contents, including: the importance of continuing care counselor psychological learners, and follow-up field to their problems, conditions, listen good horseshoe to defend himself, clarify the motives of his ac

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Publication Date
Tue Apr 01 2014
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Relationship between the microorganisms isolated from septicemic neonates and place of delivery
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Background: Neonatal septicemia is a significant cause of morbidity and mortality worldwide especially so in developing countries. To reduce the mortality caused by neonatal septicemia, it became vital to diagnose it as soon as possible and treat with administration of appropriate antibiotics.Objective: To study the relationship between themicroorganisms isolated from septicemic neonates with place of delivery.Patients and Methods: Blood sample was obtained from 76 neonates (50 of them are born in Baghdad teaching hospital (Inborn), 26 of the babies are born at home or in Al-Elwya teaching hospital (out born) ,the laboratory diagnosis for the out born patients done in the same hospital(Al-Elwya teaching hospital .The aged of the neo

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Publication Date
Thu Jan 06 2022
Journal Name
Iraqi National Journal Of Nursing Specialties
Assessment of Nurse-Midwife's Knowledge and Performance Regarding Immediate Newborn Care in Delivery Rooms at Maternity Hospitals in Baghdad City
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Objectives: To assess nurses-midwives knowledge and performance regarding immediate newborn care in delivery rooms, and to identify the relationship between nurses-midwives knowledge and performance and their socio-demographic and professional variables regarding immediate newborn care.

 

 

 

Methodology: A descriptive study was conducted from 1st of October, 2019 to 20TH of October, 2020 at three maternity teaching hospitals in Baghdad city/Al-Russafa Health Directorate: Al-Elwiya Maternity Teaching Hospital, Fatema Al- Zahra Maternity and Pediatric Hospital, Al- Za'faraniyah General Hospital. Non-probability (purposive sample) include

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Publication Date
Thu Oct 25 2018
Journal Name
Al-nahrain Journal Of Science
Synthesis of some New 3-Substituted Heterocyclic Compounds Containing Bridgehead Nitrogen from 2-Amino Pyridine with Study Their Biological Activity
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Publication Date
Mon Aug 01 2016
Journal Name
Journal Of Economics And Administrative Sciences
The Role of Moral Intelligence of Internal Auditors in Support of the Performance of Internal Audit Process - Study for Opinions of a Sample of Internal Auditors in Iraq
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Abstract

      The  aim of  the research to define the concept of moral intelligence and its dimensions and its relationship to some personal characteristics with the internal auditors and identify the importance of the employment of moral intelligence dimensions of (empathy, conscience, self- control, respect, kindness, tolerance, fairness) in the internal audit and the extent of support for the performance of the internal audit process in light of these dimensions. And that by answering the following question : Is there a role for moral intelligence  of  internal auditors in support the performance of internal audit process ? How are employ these dimensions i

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Publication Date
Mon Mar 30 2015
Journal Name
College Of Islamic Sciences
من لطائف الإسناد في صحيح البخاري: (الحديث المسلسل بالرواة الشاميين)
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  ملخّص البحث     

يهدف هذا البحث إلى التعريف بنوع من أنواع لطائف الإسناد، وهو الحديث المسلسل بالرواة الشاميين في صحيح البخاري. وأقوال العلماء فيه، ومدى موافقة أقوالهم مع شروط الحديث المسلسل بالرواة. وأهم ما جاء فيه :

  1. جرى اختيار الرواة الشاميين في صحيح البخاري ، وقد بلغ عدد الأحاديث اثني عشر حديثاً .
  2. عني شراح حديث صحيح البخاري بذكر لطائف الإسناد ، ولاسيما ابن

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Publication Date
Sun Sep 01 2019
Journal Name
مجلة العلوم القانونية
التنظيم القانوني لجريمة نقل الاعضاء البشرية من الموتى الى الاحياء
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إن عمليات نقل وزرع الأعضاء البشرية هي نوع من أنواع الأعمال الطبية، ولذلك فهي تخضع بصفة عامة لذات القواعد التي تحكم الأعمال الطبية، وقد نظمها المشرع عن طريق وضع الضوابط أو الشروط التي تكفل لتلك الممارسات عدم الخروج عن إطارها القانوني المحدد لها بالشكل الذي يضمن المحافظة على الجسم الإنسان واحترام كرامته.  وقد أكد المشرع العراقي في قانون عمليات زرع الأعضاء البشرية ومنع الاتجار بها رقم (11) لسنة 2016 على حظر

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Publication Date
Thu Jun 01 2023
Journal Name
المجلة العلمية للتربية الخاصة
الاحتراق النفسي لدى معلمي ومعلمات التلاميذ من ذوي الاحتياجات الخاصة
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Publication Date
Wed Jan 07 2026
Journal Name
Dirasat Tarbawiya
قياس جودة منهاج مادة طرائق التدريس من وجهة نظر الطالبات
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Of the most prominent elements of the educational process in physical education is a curriculum decision, which is containing a number of elements, including the book Alorteurllmadp study and which must be characterized by a high level of quality is reflected on the nature of the scientific level that will excel by the students after them with knowledge about it and to identify the vocabulary and here emerged the idea and the importance of Find Considering researchers that the quality of the textbook of the article subjects of great importance in the delivery of educational material form the right and perhaps the most prominent subjects and most important for the students of Physical Education is a substance that teaching methods and so to

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Publication Date
Wed Jan 07 2026
Journal Name
Magazine Of Historical Studies And Archaeology
المستشرق جوينبول والاستشراق الهولندي مراكزه ومدارسه وأبرز من تخرج منها
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