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STUDY THE INHIBITION ACTIVITY OF PURIFIED BACTERIOCIN FROM LOCAL ISOLATION Lactococcuslactis ssp. lactisagainst SOME PATHOGENIC BACTERIAL SPECIES ISOLATED FROM CLINICAL SAMPLES: دراسة الفعالية التثبيطية للبكتريوسين المنقى من العزلة المحلية Lactococcuslactis ssp. Lactis تجاه بعض البكتريا الممرضة المعزولة من عينات سريرية
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This study aimed to study the inhibition activity of purified bacteriocin produced from the local isolation Lactococcuslactis ssp. lactis against pathogenic bacteria species isolated from clinical samples in some hospitals Baghdad city. Screening of L. lactis ssp. Lactis and isolated from the intestines fish and raw milk was performed in well diffusion method. The results showed that L. lactis ssp. lactis (Lc4) was the most efficient isolate in producing the bacteriocin as well observed inhibitory activity the increased that companied with the concentration, the concentration of the twice filtrate was better in obtaining higher inhibition diameters compared to the one-fold concentration. The concentrated bacteriocin was purified using the gel filtration column and Sephacryl S-200. The results showed the high inhibitory activity of the purified bacteriocin after the purification against the positive and negative bacteria of the Gram stain under study compared to the one-fold concentration and two-fold before purification , The diameters of the inhibition zones after gel-filtering of the purified bacteriocin reached S. aureus, S. epidermidis, P. aeruginosa, E. coli, E. clocae and S. marcescens (23, 25, 26, 20, 22 and 28) Mm respectively. The carbohydrate content of purified bacteriocin from L. lactis ssp. lactis (Lc4) isolate was 6.02% with a molecular weight of 6310 Dalton. The results showed that purified bacteriocin retained its inhibitory activity at pH 2-10 and showed the highest inhibition at pH 4-6 and lost at pH 12. The purified bacteriocin was characterized by thermal stability. It retained its effectiveness when exposed to 40, 60, 80, 100°C for 30, 15, 5 minutes and 120°C for 15.5 minutes and lost 50% of its effectiveness when exposed to 120°C for 30 minutes. Results The purified bacteriocin was effectively retained when treated with enzyme pepsin and trypsin of 37°C for one hour and at pH 7.

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Publication Date
Mon Nov 17 2025
Journal Name
Magazine Of Historical Studies And Archaeology
المستشرق جوينبول والاستشراق الهولندي مراكزه ومدارسه وأبرز من تخرج منها
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Publication Date
Sun Jun 30 2019
Journal Name
College Of Islamic Sciences
موقف علماء المسلمين من النظريات الفلسفية المعاصرة ( المادية الجدلية أنموذجاً
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ملخـــص البحــــث

 

   تناول هذا البحث أهم وابرز النظريات الفلسفية المعاصرة وهي نظرية المادية الجدلية ، والتي اذعنت بأفكارها ان المادة هي الاساس الوحيد في اصل الوجود ، وان الموجودات مهما بلغت من مرتبة فهي دون مرتبة المادة ، وعلى هذا الاساس قامت اسسهم ، وكان للعلماء المسلمين موقفا صلبا امام هذه النظرية ، فعملوا على تفنيد هذه الاسس وابطالها بكل الوسائل المتاحة لديهم ، من

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Publication Date
Sat Aug 17 2019
Journal Name
Journal Of Economics And Administrative Sciences
الجزائر بين محاولتين من أجل التنمية: شروط النجاح وأسباب الإخفاق
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سجلت الدولة الجزائرية محاولتين من أجل التنمية، بهما أرادت القضاء على كل مخلفات الاستعمار الفرنسي، من فقر وجهل وأمراض وحرمان من أبسط ضرورات الحياة، كما عملت جاهدة على أن تكون دولة لها مكانة في صفوف الدول الصاعدة على الأقل، لما حباها الله من موارد مادية ومالية وبشرية، وموقع جيو استراتيجي، قلت مثيلة في العالم، بالنسبة لمساحتها الجغرافية الكبيرة وتنوع تضاريسها الطبيعية، وترتيبها المتقدم في الطا

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Publication Date
Thu Mar 20 1930
Journal Name
College Of Islamic Sciences
ظاهرة الغزل العذري من القراءة السياقية إلى القراءة البنيوية التكوينية
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     ملخّص البحث     

في هذا البحث عمدت الباحثة الى تحقيق ظاهرة الغزل العذري وتاصيلها ، وقد بينت آراء النقاد حول نشأته, وكان رأي الدكتور طه حسين محوراً أساسياً في بناء أغلب الآراء, فقد وافقه – دون الإشارة إليه – بعض النقاد, وخالفه بعضهم الآخر. على أنّ أغلب تلك القراءات على اختلاف رؤاها المعرفية تكاد تجمع على أن الشعراء العذريين – سواء تأكد وجودهم التأريخي أم لم يتأكد – اشترك

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Publication Date
Wed Dec 29 2021
Journal Name
Journal Of The College Of Education For Women
Impact of Electronic Games on the Increase of School Dropout among Students in the Basic Stage in Jordan from their teachers’ Perspective
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The study aims to identify the impact of electronic games on increasing the dropout rate among students in the basic stage in Jordan from their teachers’ point of view. The study adopted a descriptive survey method. Its community consisted of all fe(male) teachers of the basic stage in public and private schools, (First Amman, Irbid, and special education in Zarqa and Amman). The electronic questionnaire was used as a tool for the study. The results have shown that the effect of electronic games on increasing the dropout rate among students in the basic stage in Jordan was high. Besides, there are statistically significant differences due to the gender variable for males. There are statistically significant differences due to the varia

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Publication Date
Fri Oct 30 2020
Journal Name
Journal Of Economics And Administrative Sciences
Evaluating the reality of applying the curriculum axis according to the Iraqi academic accreditation standards / a comparative study of a sample of governmental and private universities and colleges
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The research aims to get acquainted with the evaluation of the reality of the application of the curriculum axis from among the eight Iraqi academic accreditation standards in a sample of governmental and private universities and colleges in Iraq and to identify the main and secondary reasons for it as well as to provide proposed mechanisms and procedures to help reduce gaps, If the research problem is represented in the weak availability of the requirements of the curriculum axis in universities and colleges (the study sample) due to the weak documentation and successful implementation of them and interest in them is still below the level of ambition, In order to arrive at scientific facts, the researchers adopted the comparativ

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Publication Date
Thu Jul 01 2010
Journal Name
Political Sciences Journal
العراق وسياسة حسن الجوار تجاه تركيا وايران
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العراق وسياسة حسن الجوار تجاه تركيا

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Publication Date
Wed Feb 01 2006
Journal Name
Political Sciences Journal
السياسة التركية تجاه عراق ما بعد الانتخابات
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السياسة التركية تجاه عراق ما بعد الانتخابات

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Publication Date
Mon Feb 04 2019
Journal Name
Journal Of The College Of Education For Women
الاستراتیجیة السوریة تجاه العراق بعد عام ٢٠٠٣
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The subject of this thesis acquired it's importance from many sides most
important of it , it's the nature of the relationship between Iraq-Syria relation
itself . which is basic on many close and fare relationship between them upon
the history , and also the changes that happened in global and regional area
after the 11th September 2001 and the changes of the American strategy upon
Syria , and the trying of the united states to qualify the Syrian role inside its
regional in the meddle east upon the changes that imposing by the united
states which is the most important event was the occupation of Iraq .
The importance of this subject is also due to the occupation of the united
states upon the Iraq on April 2003

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Publication Date
Tue May 16 2023
Journal Name
Political Sciences Journal
The New Russian Orientation towards the Eastern European Region after 2021
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The arrival of Russian President Vladimir Putin to power in the Russian Federation is an important factor in delineating a new approach to Russian policy towards regions that have great strategic importance affecting its national security. Therefore, NATO's progress eastward towards these regions prompts Russia to delineate a new strategy to confront this progress as well as preserving its control in the near neighboring regions represented by the countries of Eastern Europe, especially after the changes that occurred in those regions following the color revolutions that swept the region, such as the first application of this Russian policy in 2014, through the annexation of Crimea, and the latest military operations in Ukraine i

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