Preferred Language
Articles
/
jmracpc-180
Extending the storage life of some fruits by using Pullulan produced from locally isolate Aureobasidium pullulans: Extending the storage life of some fruits by using Pullulan produced from locally isolate Aureobasidium pullulans
...Show More Authors

Six isolates of A. pullulans were collected from many sources including Hibiscus sabdariffa (Roselle), old Roofs of houses and bathroom surface that referred as Ap ros1, Ap or2, 3, 4 and Ap bs5, 6 respectively, all these isolates were identified based on morphological characteristics and nutritional physiology profiles, all were able to utilize various carbon and nitrogen sources such as glucose, xylose, sucrose, maltose, ammonium sulfate, ammonium nitrate and ammonium chloride, also they showed positive test for starch and amylase, while α-cellulose, ethanol, and methanol were could not be assimilated and could not grow in 0.05% cycloheximide, the other tests referred to different results among isolates, the Ap ros1 isolate from Hibiscus sabdariffa (Roselle) was selected for production of pullulan.

           Four bacterial strains of two gram (+) and two gram (-) were chosen for the tests. The effect of pullulan coating on bacterial growth was observed in all tested strains, it was 63, 65, 70 and 75% for Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Salmonella typhimuram respectively.

The weight loss of uncoated peach was 3.2, 4.8, 6.1, 7, 7.2, 8.3 and 9.1%, while in coated peach with 10% pullulan were 3.1, 4.5, 5.8, 6.2, 6.8, 7.9 and 8.9%, and in coated peach with 20% pullulan were 2.9, 3.8, 4.1, 4.6, 5.8, 6.4 and 7.6%, when these samples are stored at 3, 6, 9, 12, 15, 18 and 21 days respectively that stored at 25°C, and when uncoated and coated peach with 10 and 20% pullulan stored at 4°C . The weight loss in uncoated peach was 0.01, 0.06, 0.16, 0.23, 0.35, 0.46, 0.6, 0.68, 0.73 and 0.75%, while in coated peach with 10% pullulan were 0.01, 0.05, 0.12, 0.16, 0.2, 0.3, 0.5, 0.58, 0.64 and 0.71%, and in coated peach with 20% pullulan were 0.015, 0.02, 0.04, 0.08, 0.1, 0.12, 0.18, 0.3, 0.46 and 0.5%, when these samples are stored at 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 days respectively.

The weight loss of uncoated pear was 1.4, 2, 2.6, 4.4, 5.5, 6.4 and 7.2%, while in coated peach with 10% pullulan were 1.2, 1.6, 2, 2.4, 4.3, 5.2 and 6.6%, and in coated peach with 20% pullulan were 0.8, 1, 1.4, 1.6, 3, 4.6 and 5.4%, when these samples are stored at 3, 6, 9, 12, 15, 18 and 21 days respectively that stored at 25°C, and when uncoated and coated pear with 10 and 20% pullulan that stored at 4°C. The weight loss is 0.02, 0.07, 0.14, 0.16, 0.3, 0.33, 0.44, 0.45, 0.52 and 0.54%, while in coated peach with 10% pullulan were 0.02, 0.06, 0.11, 0.16, 0.21, 0.3, 0.36, 0.38, 0.44 and 0.46%, and in coated peach with 20% pullulan were 0.01, 0.05, 0.08, 0.09, 0.1, 0.16, 0.19, 0.24, 0.26 and 0.28%, when these samples are stored at 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 days respectively.

View Publication Preview PDF
Quick Preview PDF
Publication Date
Tue Apr 30 2024
Journal Name
Iraqi Journal Of Science
The Influence of Biologically Synthesized Copper Nanoparticles on the Biofilm Produced by Staphylococcus haemolyticus 1solated from Seminal Fluid
...Show More Authors

     Staphylococcus haemolyticus is one of the most frequently isolated coagulase-negative staphylococci. The ability to form biofilm is considered as one of the most important virulence factors of coagulase negative staphylococci. There is only limited knowledge of the nature of S. haemolyticus biofilms. This study was aimed at evaluating the ability of S. haemolyticus strains to produce biofilm in the presence of copper oxide nanoparticles (CuONPs). The biological synthesis of nanoparticles is an environmentally friendly approach for large-scale production of nanoparticles. Copper oxide nanoparticles were produced in the current study from the S. haemolyticus viable cell filtrate. UV-visible (UV-Vis) spectroscopy, X-ray diffra

... Show More
Scopus (1)
Scopus Crossref
Publication Date
Sun Aug 13 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Purification of algnic acid by Local isolate of Azotobacter Vinelandi
...Show More Authors

The bacteria Azotobacter Vinelandii  was taken from a central research in Baghdad, The purification of alginic acid which produced from the bacteria by several steps starting with precipitation with isopropanol (3:1) v/v , Washing by ppt with 100ml of isopropanol       : distilled water (3:1) v/v , then the ppt was dissolved in warm distilled water and dialysis against distilled water from 24 h/s . To Complete the purification , gel filtration chromatography was conducted on sephacryl s-100 column followed by ion – exchange chromatography . Using DEAE cellulose column . The molecular Weight of purified al ginic acid was higher than that of blue dextran 2000,It was more than (2) millions Dalton .<

... Show More
View Publication Preview PDF
Publication Date
Wed Jun 01 2022
Journal Name
Baghdad Science Journal
Evaluation of some Virulence Factors and Drug Resistance of Bacteria Isolated from the Urine of Patients with TCC-Bladder Cancer
...Show More Authors

Urinary tract infections (UTIs) mean microbial pathogens in the urethra or bladder (lower urinary tract). Important risk factors for recurrent UTI include obstruction of the urinary tract, use of a bladder catheter or a suppressed immune system. This study aims to isolate and identify bacteria from patients with TCC-bladder cancer or patients with a negative cystoscope and estimate antibiotic susceptibility patterns and evaluate some of the virulence factors. From a total of 62 patients with TCC-BC or negative cystoscope, only 35 favorable bacterial growths were obtained, including Escherichia coli (UPEC), a significant bacterial isolate, and Stenotrophomonas maltophilia. The percentage of multi drug-resistance bacteria

... Show More
View Publication Preview PDF
Scopus (3)
Crossref (2)
Scopus Clarivate Crossref
Publication Date
Sat Jun 01 2019
Journal Name
Journal Of Economics And Administrative Sciences
Using Some Robust Methods For Handling the Problem of Multicollinearity
...Show More Authors

The multiple linear regression model is an important regression model that has attracted many researchers in different fields including applied mathematics, business, medicine, and social sciences , Linear regression models involving a large number of independent variables are poorly performing due to large variation and lead to inaccurate conclusions , One of the most important problems in the regression analysis is the multicollinearity Problem, which is considered one of the most important problems that has become known to many researchers  , As well as their effects on the multiple linear regression model, In addition to multicollinearity, the problem of outliers in data is one of the difficulties in constructing the reg

... Show More
View Publication Preview PDF
Crossref
Publication Date
Wed Jun 29 2016
Journal Name
Iraqi Journal Of Market Research And Consumer Protection
Effect of adding the Flavonoids from Zizypus spina-christi leaves in redox indicators of the fat extracted from Cows, Sheeps bones and storage on defferent temperature and periods.: Effect of adding the Flavonoids from Zizypus spina-christi leaves in redox indicators of the fat extracted from Cows, Sheeps bones and storage on defferent temperature and periods.
...Show More Authors


Flavonoids were extracted from Zizyphus spina-christi leaves by Ethyl acetate after acid digested and used as antioxidant. The dried extract was added separately to each sample of fat extracted from hallow cow and sheep bones as follows: T1 cow fat, T2 control for cow fat, T3 sheep fat and T4 control for sheep fat (the control T2 and T4 reffered to samples without added antioxidant).
Samples were stored at -18, 5, 25 and 55 °C for 28 days. The storage trials were conducted at -18, 5 and 25 °C for 28 days for T1, T2, T3 and T4. The chemical indices examined initially and at the end of storage period. PVs was 1.46, 1.46, 1.8 and 1.8 meq/ Kg oil respectively, FFA values were 0.245, 0.245, 0.244 and 0.244% respectively and TBA va

... Show More
View Publication Preview PDF
Publication Date
Sun Jan 01 2017
Journal Name
Journal Of Engineering
Removal of Copper from Simulated Wastewater by Applying Electromagnetic Adsorption for Locally Prepared Activated Carbon of Banana Peels
...Show More Authors

The adsorption of copper ions onto produced activated carbon from banana peels (with particle size 250 µm) in a single component system with applying magnetic field has been studied using fixed bed adsorber. The fixed bed breakthrough curves for the copper ions were investigated. The adsorption capacity for Cu (II) was investigated. It was found that 1) the exposure distance (E.D) and strength of magnetic field (B), affected the degree of adsorption; and 2) experiments showed that removal of Cu ions and accumulative adsorption capacity of adsorbent increase as the exposure distance and strength of magnetic field increase.
 

View Publication Preview PDF
Publication Date
Tue Dec 15 2015
Journal Name
Iraqi Journal Of Market Research And Consumer Protection
Determination of some Optimum Conditions for Bioremediation of Some Heavy Metals by Saccharomyces cerevisiae: Determination of some Optimum Conditions for Bioremediation of Some Heavy Metals by Saccharomyces cerevisiae
...Show More Authors

The study aimed to determine of some Optimum conditions for bioremediation and removing of seven mineral elements included hexavalent chromium, nickel, cobalt, cadmium, lead, iron and copper as either alone or in group by living and heat treated cells of baker’s yeast Saccharomyces cerevisiae. The dried baker's yeast from Aldnaamaya China Company was used in this study. Biochemical tests was used to ensure yeast belonging to S. cerevisiae and then used to remove the mentioned mineral elementes under different conditions which included incubation period, pH, and temperature. It was found that the best of these conditions was 60 minutes for duration of incubation, 6 for pH, 25 ᵒC for temperature. During the study the behavior of living

... Show More
View Publication Preview PDF
Publication Date
Thu Mar 07 2013
Journal Name
International Journal Of Pharma Sciences
Separation and Purification of Hemolysin from Local Isolate of Serratia marcescens
...Show More Authors

Twenty isolates of Serratia marcescens were isolated from inflammation of the urinary tract (UTI)., These isolates were found to produce hemolysin as indicated by blood agar plates in which the hemolysis of red blood cell indicate a positive result. Isolates were selected according to their hemolysis activity by measuring absorbance of hemoglobin at 405 nm that released from red blood cell. Hemolysin was completely purified using 50-75% saturation of ammonium sulphate followed by ion exchange chromatography with DEAE-cellulose then gel filtration chromatography by sepharose 4B. Accordingly molecular weight for the purified toxin was estimated as 45 KD.

Publication Date
Sun Sep 01 2013
Journal Name
Baghdad Science Journal
Purification and Characterization of Endoglucanase from local isolate of Aspergillus flavus
...Show More Authors

Endoglucanase produced from Aspergillus flavus was purified by several steps including precipitation with 25 % ammonium sulphate followed by Ion –exchange chromatography, the obtained specific activity was 377.35 U/ mg protein, with a yield of 51.32 % .This step was followed by gel filtration chromatography (Sepharose -6B), when a value of specific activity was 400 U/ mg protein, with a yield of 48 %. Certain properties of this purified enzyme were investigated, the optimum pH of activity was 7 and the pH of its stability was 4.5, while the temperature stability was 40 °C for 60 min. The enzyme retained 100% of its original activity after incubation at 40 °C for 60 min; the optimum temperature for enzyme activity was 40 °C.

View Publication Preview PDF
Crossref
Publication Date
Sat Mar 27 2021
Journal Name
مجلة دراسات ويحوث التربية الرياضية
Hypoxic exercises by using (training mask) and its effect on the (PMA) and some physiological indicators and achievement for 1500m runners
...Show More Authors

Hypoxic training, which in turn is one of the methods adopted in sports training methods, especially in activities that depend on the aerobic system in its performance, which includes training with a lack of oxygen by reducing its molecular pressure, since this method targets functional organs and works temporary responses during training and permanent responses After training as an adaptation to these devices as a result of training in this way, the study aimed to identify the effect of hypoxic exercises using the training mask and the extent of the change in some biochemical indicators, in addition to that to identify the effect of these exercises on the indicator of energy expenditure and )VMA) and the achievement of the effectiveness of

... Show More
View Publication Preview PDF