This study was conducted in the plant protection department/ College of Agriculture/ University of Baghdad to evaluate the efficiency of physical agents ozone, ultraviolet radiation, microwave for destroying afla produced in corn seeds. An isolate af A.flavus producing Aflatoxin B1 was obtained from plant protection dept. college of Agric. University of Baghdad. Results showed destroy toxin AFLA B1 the effect of radiation microwave in the media of Japex degree 80 and 100 c^o 57.14% and 85.71%, respectively, and for 20 sec, compared to the treatment comparison 0.00% as found significant differences were apparent between transactions and the treatment of comparison, as and notes the existence of a significant difference to the treatment radiation microwave degree 100 c^o, amounting to reduction ratio of 85.71%, the results of the effect of radiation microwave showed a destroy AFLA B1 crude by two notches 80 and 100 c^o, times 5, 10, 20 sec, and there are significant differences between treatments and treatment comparison as well as the existence of clear differences of treatments degree 80 and 100 c^o and time of 20 sec and rates reduced 95.09% and 91.18%, respectively, showed the results of destroy toxin AFLA B1 contaminated corn and the presence of significant differences apparent between the comparative treatment and the rest of the transactions when it was noted the lack of significant differences between transactions ozone and ultraviolet in 240.365 nm and radiation microwave degree effect temperature 80 and 100 c^o and rates reduced 89.52% and 89.52% and 69.52% and 94.29% and 89.52%, respectively, and was the best treatment is radiation microwave degree 80 m and by the reduction of 94.29%, and the results showed that there were significant differences apparent between the comparison treatment non-polluting and the rest of the transactions and observed significant differences were apparent between transactions radiation microwave and ozone and ultraviolet radiation led by microwave treatment of degrees effect 80 and 100 c^o and by the reduction of 100% for both degrees.