The isolates were screened according to their capability for pectinase production, screening process identified the best pectinolytic isolate and it was characterized by cultural and biochemical, as Pesudomonas sp. Pectinolytic enzyme producing bacterium Pesudomonas sp. was isolated from the Iraqi soil on nutrient agar plate. Optimiztion of process parameters were carried out by altering some of environmental conditions of chemo-physical environment for the production medium. The highest pectinase production was observed at 48 hrs of incubation at 35 °C with the initial pH of 6.0. Different nutrients and environmental conditions were investigated in terms of their effect on the production of extracellular pectinase using citrus pectin a

In the leaves of Olea europaea L. Olive trees an endophytic fungus was discovered. Cladosporium sp. was identified to be the fungus based on its morphological characteristics and nuclear ribosomal DNA ITS sequence analysis and was registered in NCBI as the Cladosporium genus has been registered under the number (0P939922.1) The species was not specified, and it was considered of unknown species after comparing it to global isolates. In comparison to olive leaf extract, Cladosporium sp. including total flavonoid, total phenolic, total terpenoid, and total saponins, Which were 121.9%, 198.1%, 89.13%, and 29.87 % respectively compared to its content in olive leaf extract, which was 61.54 %, 67.88 % , 17.1

 The study was conducted to isolate and identify endophytic fungi from leaves and twigs of lebbeck (Albizia lebbeck) and study their antagonistic activity against some plant fungal pathogens. Results showed isolation of 75 isolate endophytic fungi from 240 segments of leaves and twigs representing colonization frequency of 47.43%. Isolated fungi included different species of Aspergillus which prevail over other species (6 species), and three different isolates of Penicillium, one more species of Paecilomyces, Drechslera, Fusarium, Curvularia, Nigrospora and hyaline sterile fungus. Results showed variation in colonization frequencies within all species isolated from the two plant parts.  Dual culture methods for

Endophytic fungi live inside plants or any part of them without creating any visible pathogenic signs. Endophytic fungi are found within medicinal plants and have shown strong biologic activity, such as anticancer and antioxidant activities, as well as producing extracellular enzymes. In this study, different fungal strains were isolated from the leaves of the medicinal plant Ziziphus spina, including Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Cladosporium sp., Rhizopus sp., and Mucor sp. Extracellular enzymes have been quantified using agar plate-based methods in which fungi were grown in specified growth media to detect the enzymes produced. The results showed that A. niger has the highest ability to produce amy

The current study aims to produce cellulase enzyme from Streptomyces spp. isolates and study the effect of some cultural conditions on cellulase production; biofuel production from cellulotic waste through enzymatic and acids hydrolysis. Out of 74 isolates of Streptomyces sp. were screened for cellulse production in solid and liquid media. Results showed higher capability of isolate Streptomyces sp. B 167 for cellulase production and bioconversion of cellulose, therefore selected for further studies. The results of optimization revealed that the cellulase enzyme productivity by the selected isolate reached 2.1 and 2.28 U/ml after 48 h of incubation time and pH 7 respectively. Cellulase productions in tested isolate improved (2.57 U/ml) b

The current study aims to produce cellulase enzyme from Streptomyces spp. isolates and study the effect of some cultural conditions on cellulase production; biofuel production from cellulotic waste through enzymatic and acids hydrolysis. Out of 74 isolates of Streptomyces sp. were screened for cellulse production in solid and liquid media. Results showed higher capability of isolate Streptomyces sp. B 167 for cellulase production and bioconversion of cellulose, therefore selected for further studies. The results of optimization revealed that the cellulase enzyme productivity by the selected isolate reached 2.1 and 2.28 U/ml after 48 h of incubation time and pH 7 respectively. Cellulase productions in tested isolate improved (2.57 U/ml) b

  The study was aimed to isolate and identify endophytic fungi from myrtle (Mrtus communis) leaves and study their extracellular enzymes activity. Results revealed isolation of 99 species or ioslated of endophytic fungi obtained from 500 leaves fragment, which represented colonization frequency (CF) of 20.4%. Those fungi were related to 23 species or isolates, Aspergilli were dominated over all species (11 species) with CF 14%. Moreover A. niger and A. flavus showed the higtest CF values over all. Other isolated fungi were related to the genera: Penicillium spp., Cladosporium sp., Cunninghamella sp., Derchslera sp., Alternaria sp. Paecilomyces sp. and sterile fungi (mycelia sterilia). Extracelluar enzymes activity showed varation in

Cladosporium sp. plays an important role in human health, it is one of the pathogenic fungi which cause allergy and asthma and most frequently isolated from airborne spores.  In this study, a couple of universal PCR primers were designed to identify the pathogenic fungi Cladosporium sp. according to conserved region 5.8S, 18S and 28S subunit ribosomal RNA gene in Cladosporium species. In silico RFLP-PCR were used to identify twenty-four Cladosporium strains. The results showed that the universal primer has the specificity to amplify the conserved region in 24 species as a band in virtual agarose gel. They also showed that the RFLP method is able to identify three Cladosporium spe

Biologically active natural compounds are molecules produced by plants or plant-related microbes, such as endophytes. Many of these metabolites have a wide range of antimicrobial activities and other pharmaceutical properties. This study aimed to evaluate (in vitro) the antifungal activities of the secondary metabolites obtained from Paecilomyces sp. against the pathogenic fungus Rhizoctonia solani. The endophytic fungus Paecilomyces was isolated from Moringa oleifera leaves and cultured on potato dextrose broth for the production of the fungal metabolites. The activity of Paecilomyces filtrate against the radial growth of Rhizoctonia solani was tested by mixing the filtrate with potato dextrose agar medium at concentrations of 15%,