Background: The discriminative power of the classical WHO parameters in relation to male fertility is quite low, because they only address few aspects of sperm quality and function. This has led investigators to focus their attention on the male gamete and in particular its genome.Objective: To explore which of the sperm DNA damage parameters measured by comet assay are more reliable, and their relations with the standard semen parameters.Methods: Study was done on 40 infertile men selected from couples attending the Institute of Embryo Reasearch and Infertility Treatment at Al-Kadhimiya City/ Baghdad in the period between February 2009 and May 2009, with a history of infertility of ≥1 years; and 15 healthy volunteers of proven fertility serving as control. Samples were allowed to liquefy for at least 30 minutes at 37°C and then evaluated according to the guidelines of the World Health Organization, 1999. The single cell gel electrophoresis (comet) assay was studied in the ejaculated spermatozoa. The exclusion criteria were the presence of azoospermia and severe oligospermia. Results were compared with the standard semen characteristics (concentration, motility and morphology).Results: Compared to healthy volunteers, infertile patients had highly significantly higher values of comet parameters (P = 0.00001). In all infertile patients, comet extent did not correlate with any of the classical semen parameters; whereas, tail length (μm), % DNA in tail, tail moment and olive moment had all significant negative correlations with the standard parameters. Significant positive correlations were observed between the studied comet parameters, except for the comet extent which was not significantly correlated with tail length and % DNA in tail (P = 0.06, P = 0.7; respectively).Conclusion: Comet assay is a very useful technique in assessing sperm DNA damage. Comet tail parameters and comet extent, may clarify different aspects of DNA damage, and together give a better insight to the integrity of the male genome.Keywards: sperm, comet extent, % DNA in tail, tail moment and olive moment
خلفية البحث: المتلازمة الأيضية عند المرضى العراقيين المصابين بالمتلازمة التاجية الحادة قليلا ما تمت دراستها. الأهداف: دراسة الخصائص المجتمعية-السكانية للمرضى العراقيين المصابين بالمتلازمة الايضية مع المتلازمة التاجية الحادة. المرضى وطرق العمل: شملت الدراسة المقطعية 150 مصابا بالمتلازمة التاجية الحادة الذين يعالجون في وحده العناية القلبية في مستشفى اليرموك التعليمي في بغداد للفترة من منتصف كانون الث
... Show MoreAim: The study designed to evaluate the Geno-protective effect of green tea extract against genotoxicity induced by metronidazole and tinidazole. Methods: Thirty-six mice were used, For each experiment, The animals divided into 6 groups: Group I- Negative control administered distilled water; Group II-Healthy mice treated with metronidazole alone, Group III- Healthy mice treated with tinidazole alone; Group IV- Healthy mice administered green tea extract alone Group V- Healthy mice treated with metronidazole, followed by green tea extract administration, Group VI- Healthy mice treated with tinidazole, followed by administration of green tea extract. Results: treatment with Tinidazole significantly increase total chromosomal aberration (0.18
... Show MoreManganese sulfate and Punica granatum plant extract were used to create MnO2 nanoparticles, which were then characterized using techniques like Fourier transform infrared spectroscopy, ultraviolet-visible spectroscopy, atomic force microscopy, X-ray diffraction, transmission electron microscopy, scanning electron microscopy, and energy-dispersive X-ray spectroscopy. The crystal's size was calculated to be 30.94nm by employing the Debye Scherrer equation in X-ray diffraction. MnO2 NPs were shown to be effective in adsorbing M(II) = Co, Ni, and Cu ions, proving that all three metal ions may be removed from water in one go. Ni(II) has a higher adsorption rate throughout the board. Co, Ni, and Cu ion removal efficiencie
... Show MoreThe depletion of petroleum reserves and increasing environmental concerns have driven the development of eco-friendly asphalt binders. This research investigates the performance of natural asphalt (NA) modified with waste engine oil (WEO) as a sustainable alternative to conventional petroleum asphalt (PA). The study examines NA modified with 10%, 20%, and 30% WEO by the weight of asphalt to identify an optimal blend ratio that enhances the binder’s flexibility and workability while maintaining high-temperature stability. Comprehensive testing was conducted, including penetration, softening point, viscosity, ductility, multiple stress creep recovery (MSCR), linear amplitude sweep (LAS), energy-dispersive X-ray spectroscopy (EDX), F
... Show MoreThis study looks into the many methods that are used in the risk assessment procedure that is used in the construction industry nowadays. As a result of the slow adoption of novel assessment methods, professionals frequently resort to strategies that have previously been validated as being successful. When it comes to risk assessment, having a precise analytical tool that uses the cost of risk as a measurement and draws on the knowledge of professionals could potentially assist bridge the gap between theory and practice. This step will examine relevant literature, sort articles according to their published year, and identify domains and qualities. Consequently, the most significant findings have been presented in a manne
... Show MoreThe geochemical study of the Oligocene-Miocene succession Anah, Euphrates, and Fatha formations, western Iraq, was carried out to discriminate their depositional environments. Different major and trace patterns were observed between these formations. The major elements (Ca, Mg, Fe, Mn, K, and Na) and trace elements (Li, V, Cr, Co, Ni, Cu, Zn, Ga, Rb, Sr, Zr, Cs, Ba, Hf, W, Pb, Th, and U) are a function of the setting of the depositional environments. The reefal facies have lower concentrations of MgO, Li, Cr, Co, Ni, Ga, Rb, Zr, and Ba than marine and lagoonal facies but have higher concentrations of CaO, V, and Sr than it. Whereas dolomitic limestone facies are enriched V, and U while depletion in Li, Cr, Ni, Ga, Rb, Sr, Zr, Ba, an
... Show MoreThere are a few studies that discuss the medical causes for diabetic foot (DF) ulcerations in Iraq, one of them in Wasit province. The aim of our study was to analyze the medical, therapeutic, and patient risk factors for developing DF ulcerations among diabetic patients in Baghdad, Iraq.