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Sensitivity of Bacteria Escherichia coli Iisolated from Urinary Tract Infection toward Ciprofloxacin and Lomefloxacin.
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   Four hundred and fifty urine samples were collected from patients suffering from urinary tract infection from the General Azadi hospital in Kurkuk province ,during the period of october 2007 till march 2008 .       Results of bacteriological culture revealed that (168) out of (450) studied samples (37.3%) gave positive culture using blood agar and macConkey agar ; different species of bacterial isolates were detected using morphological and biochemical tests ,from these isolates the highest percentage of the isolates were from Escherichia coli when it was (100) isolates out of (190) isolate  (52.63%)  .  one hundred isolates were distributed between (77) from females and (23) from males     The susceptibility  of E.coli isolates towards (13) types of antimicrobial agents commomly used in treatment of urinary tract infection were tested in vitro . Variations in susceptibility against antimicrobial were found. The antiseptic drug Nitrofurantoin showed the highest activity on E.coli when the resistance reached  (14 %) , from the other hand E.coli isolates showed great resistance to other antimicrobial : sulphamethoxazole was (100 %) ,Ampicillin (99 %) ,Cephalexin   (62 %) ,Agumentin (58 %) , Nalidixic acid (54 %) , Trimethoprim (52 %) , Pipracillin (45 %) , Ceftriaxone (40 %) , Lomefloxacin (39 %) , Cefotaxime (39 %) , Ciprofloxacin (37 %) and to Co-trimxazole was (37 %) .     Minimal inhibitory concentration (MIC) for Ciprofloxacin and Lomefloxacin were studied and the results showed higher values in MIC. MIC values for eight  isolates of bacteria  E.coli were so high they were (400> µg/ml) for Ciprofloxacin were (19.5%) while five isolates of bacteria E.coli were with  MIC value of (400>µg/ml) with (11.6%) to Lomefloxacin .        The ability of bacteria E.coli isolates  to produce ß-lactamase enzyme using Capillary tubes method was studied. The results showed (61 %) of these isolates gave positive results when the red cloer of the indicator phenol was changed from red to yellow within (15) minuets .When using Disc approximation method to detect the ability to production Extended Spectrum ßlactamase enzyme (ESBLs) out of (61)isolates only (11)isolates were positive (18 %); Nine ESBLs producer showed high resistance to antimicrobial Quinolones (Naldixic acid ,Ciprofloxacin and Lomefloxacin) with percentage reached (72.7%) . 

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Publication Date
Wed Mar 10 2021
Journal Name
Baghdad Science Journal
Gel clot assay used for detection of candida spp onfection in urinary tract
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the study including isolation and identification of candida spp causing UTIs from patintes coming to al-yarmouk hospital

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Publication Date
Wed Dec 01 2021
Journal Name
Iraqi Journal Of Veterinary Sciences
Isolation and antimicrobial resistance of Staphylococcus spp., enteric bacteria and Pseudomonas spp. associated with respiratory tract infections of sheep
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Sheep are considered as an important part of livestock in the worldwide, particularly in Iraq, as they provide meat, milk, leather, wool, and manure. The present study aim is isolation and identification of staphylococci, enteric bacteria and Pseudomonas spp. Totally, 115 samples were collected from sheep (100 samples were collected from the nasal cavity of local sheep suffering from respiratory infections, and 15 samples were collected from apparently healthy local sheep). All the samples were collected from seven flocks located in Abu Ghraib and Al-Radwaniyah, Baghdad governorate, Iraq. The samples were taken during the period from October 2020 to February 2021. Staphylococcus spp., Pseudomonas spp., and enteric bacteria were detected fi

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Publication Date
Thu Jul 20 2023
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Detection of antibiotic resistance of the phylogenetic group E among E. coli bacteria isolated from diarrheal cases in children under five years
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From 50 stool samples collected from children with diarrhea of both sexes who visited various hospitals in Baghdad, 26 isolates of E.coli were found to belong to the phylogenetic group E. The findings revealed that the percentage of E.coli for thephylogenetic group E is (52%) , making it the dominant group among the other phylogenetic groups. The findings demonstrated that 100% of the E.coli isolates from phylogenetic group E are resistant to penicillin, and only 15% are resistant to imipenem. Multi-drug resistance (MDR) was found to be 15%, while XDR reached 85%. The results of thephylogenetic group for the remaining species of isolates in this study were group A (2/50 and by 4%), gr

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Publication Date
Tue Dec 01 2020
Journal Name
Meta Gene
Association between the rs2234671 polymorphism and the risk of recurrent urinary tract infections in Iraqi women
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A case-control study was designed to find out the association between rs2234671 polymorphism of cxcr1 and rUTI in a sample of Iraqi women by polymerase chain reaction- sequence-specific primer (PCR-SSP) method. The current findings revealed that the genotype GC (OR= 7.86, 95% CI = 2.82-21.87, P= 7.7 × 10-5) and the C allele (OR= 3.93, 95% CI = 1.97 - 7.83, P = 9.8×10-5) are significantly associated with rUTI. However, the genotype GG played as a protective factor (OR= 0.12, 95% CI = 10.05 - 0.34, P = 4.0 ×10-5). Depending on these findings, the genotype GC is significantly associated with rUTI.

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Publication Date
Thu Oct 01 2020
Journal Name
Biochemical & Cellular Archives
THE STUDY ON ABILITY OF ESCHERICHIA COLI ISOLATED FROM DIFFERENT CLINICAL CASES TO BIOFILM FORMATION AND DETECTION OF CSGD GENE RESPONSIBLE FOR PRODUCE CURLI (FIMBRIAE)
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A total of 165 clinical sample included Urine, Swab wounds and Burns were collected from Baghdad Governorate. Results showed that rate all isolates of E. coli was 50(30.3%) and rate of urine infection was 46(92%) and rate of swab wounds infection 4(8%). Where was diagnostic based on streaked on MacConkey agar, then single colony was transferred to Eosin Methylene Blue (EMB). Identification some of the biochemical test included: Catalase test, Oxidase test, Indole test, Methyl red, Vogues - Proskauer test and Citrate Utilization test. Then confirmed by the Vitek - 2 Compact System. The ability of E.coli isolate to biofilm formation to be studied it is considered one of the most important factors of virulence and has role in causing injury an

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Publication Date
Thu Jun 30 2016
Journal Name
Al-kindy College Medical Journal
Prevalence of bacteria isolated from patients with burn infection at a burn hospital in Baghdad and study of their antimicrobial susceptibility patterns
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Background: The skin functions as a barrier to the external environment, damage to this barrier following a burn disrupts the innate immune system and increases susceptibility to bacterial infection. Objective: This study was carried out to determine the bacterial isolates and study their antimicrobial susceptibility in burned wound infections at one burn's hospital in Baghdad.Type of study:Cross-sectional study.Methods: The bacteria were identified at species level by using Analytic Profile Index (API) system and The antimicrobial susceptibility test was performed according to Kirby-Bauer (disk diffusion) technique.Results: Over a period of one year (from October 2014 to October 2015). Out of 848 patients with different degrees of burns

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Publication Date
Wed Jan 01 2020
Journal Name
Euromediterranean Biomedical Journal
ESCHERICHIA COLI O157: H7 INVESTIGATION IN CHILDREN WITH DIARRHEA: AN IRAQI CROSS-SECTIONAL STUDY
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NA Nasir, SHM Ali, HQMA AL-Ess, WA Hussein, MKW Al-Janabi, KIA Mohammed, JM Mosa, Euromediterranean Biomedical Journal, 2020

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Publication Date
Fri Feb 08 2019
Journal Name
Iraqi Journal Of Laser
Urinary Tract Stones Fragmentation using (2100 nm) Holmium: YAG Laser: (In vitro Analysis)
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Urinary stones are one of the most common painful disorders of the urinary system. Four new technologies have transformed the treatment of urinary stones: Electrohydraulic lithotripsy, ultrasonic lithotripsy, extracorporeal shock wave lithotripsy, and laser lithotripsy.The purpose of this study is to determine whether pulsed holmium laser energy is an effective method for fragmenting urinary tract stones in vitro, and to determine whether stone composition affects the efficacy of holmium laser lithotripsy. Human urinary stones of known composition with different sizes, shapes and colors were used for this study. The weight and the size of each stone were measured. The surgical laser system which used in our study is Ho:YAG laser(2100nm)

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Publication Date
Wed Jul 01 2020
Journal Name
Indian Journal Of Forensic Medicine And Toxicology,
Effects of alcoholic extracts of cinnamomum zeylanicum and origanum majorana on expression of hly gene in escherichia coli
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Scopus
Publication Date
Tue Jan 01 2013
Journal Name
Bio-genetics Journal
Measuring the toxicity of Heat Stable Enterotoxin (a) produced by Enterotoxigenic Escherichia coli on human blood lymphocyte from normal and colorectal cancer patients in vitro
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This research was conducted to measure the safety of heat stable enterotoxin a (STa) produced by enterotoxigenic Escherichia coli, through studying its toxic effect on human blood lymphocyte, since it showed a promising effect in reducing the proliferation of colorectal cancer cells. the cytogenetic effects of (STa) by using five different concentrations (100, 200, 400, 800 and 1600μg/ml) in comparison with negative (PBS, Phosphate buffer saline) and positive (MMC, Mitomycin C) at concentration of 5μg/ml, controls on human blood lymphocytes obtained from both (10) normal healthy persons and (20) colorectal cancer patients was measured by employing the following parameters: mitotic index, blast index, chromosomal aberrations and micronucle

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