The present study aimed to try to find natural substances stimulate the production of bacteriocin, as well as "for detection of bacteriocin producing isolates. Two hundred and eighty ( 280) bacterial isolates, gram negative only, were collected from 760 different pathogenic samples, consist: (Urinary tract infection, septicemia, Vaginal inflammation and diarrhea). The isolated bacteria are: Escherichia coli, Klebsiella pneumonia Pseudomonas aeruginosa,, Salmonella typhi, Enterobacter cloacae, Acinetobacter baumannii, Serratia liquefaciens, Citrobacter freundii, Proteus mirabilis and Serrattia odorifera. Cup assay method was used to detect bacteriocin production. Locally media prepared ( Nutriernt agar + Brassica rapa roots extract ) to detect bacterial bacteriocin production, compared with ( N. agar ) only. The results showed, the percentage of bacteria production of bacteriocin were (28.57%)/(80) isolates only on N. agar, while the ratio reached to (82.5%)/ (231) isolates by local media.Also this media gave (45 mm) in dimeter of inhibition zone in E. coli. Brassica rapa roots extract was used to stimulate bacteriocin production compared with mitomycin-c (Mt-c ) in five isolates of the E. coli. It was found the extract emulate Mt-, in dimeter of inhibition zone , protein concentration and activity. But it was better than Mt-c in some isolates.
1 - is not affected by illiteracy cells painful eggs after the first and seventh of the various concentrations used but found the effect of 21 and 35 days after treatment2 - repeat chromosomal aberrations illiteracy eggs cells no different distortions occurring sperm cells During Altnavra phase3 - increased chromosomal aberrations increase the dose especially for 21 and 35 days4 - The connective tissue is more sensitive phase of the pesticide from Altnavra phase
The aim of this study was the production of aspartame by using immobilized thermolysin in bentonite clay. The yield of immobilized thermolysin in bentonite was 92% of the original enzyme amount. pH profile of free and immobilized enzyme was 7.0 and 7.5 respectively which was stable at 6.5-9.0 for 30min. The optimum temperature of both enzymes was 50°C, while they were stable at 65°C for 30min. however, they lost 52.73 and 61.72% from its main activity at 80°C respectively. Immobilized thermolysin has retained all activity within 27 days, but it kept 68.27% of initial activity when stored for 60 days at 4°C whereas, it retained a full activity after 20 continue usage. In addition, it retained 86.53% of its original activity af
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The current study was conducted on 504(Ros-308) broiler chicks during the period 28/9/2017-9/11/2018to determine the effect of heat shock in early age and additives such as ginseng in three levels on birds weight and feedconsumption. Results showed that the exposure to high temperature (38-400C) lead to significant decrease (p≤≤≤≤≤0.05 (inaverage body weight at7 day of age and significant decrease in body weight in birds expousured to high temperature inthe periods 2, 4 and 6 hours compared with control (Table 1). Significant decrease in live body weight when exposure to2hr compared with 6hr namely (138.54) and (144.21), respectively while no significant difference between 2 and 4h.Results showed no significant effect in body we
... Show MoreBackground: Bacteriocin is a peptidic toxin has many advantages to bacteria in their ecological niche and has strong antibacterial activity. Objective: The aim of this study was to evaluation of bacteriocin using Streptococcus sanguinis isolated from human dental caries.
Subjects and Methods: Thirty five streptococcus isolates were diagnosed and tested for their production of bacteriocin, and then the optimal conditions for production of bacteriocin were determined. After that, the purification of bacteriocin was made partially by ammonium sulfate at 95% saturation levels, followed by and gel filtration chromatography
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Seeds of the two rice genotypes namely Amber 33 (A33) and Amber Baghdad (AB) were divided into two groups; the first was presoaked in different concentrations of ethyl methane sulphonate (EMS) as chemical mutagen for different duration times (3, 6 and 12) hrs, and the other was exposed to different exposure times of ultra violate (UV-B) radiation (280-320 nm) as physical mutagen for different times (20, 40 and 60) min at room temperature. Treated and non-treated seeds were transferred into the callus induction medium containing 2.5 mg/L 2,4- dichlorophenoxy acetic acid (2,4-D) and 0.5 mg/L benzyl adenine (BA) under aseptic conditions. Calli were divided into two groups the first was treated with several EMS concentrations (0.0, 0.50. 1.0, 1
... Show MoreIn this study, detection of uricase production from Pseudomonas aeruginosa
isolates was done by applying colorimetric method, Uricase was purified from the
most potent isolate by precipitation using ammonium sulphate (80% saturation) then
purification was achieved using DEAE –Cellulose ion exchange and Sepharose 6B
gel filtration chromatography column, 16.4% of total enzyme was recovered with
specific activity 2337.5U/mg and 22.21folds of purification. Characterization of
uricase involved detection of optimal conditions for uricase activity, the maximal
activity was obtained at temperature 45ºC,while uricase appeared to be stable at
40ºC. Uricase showed optimal activity at pH 9 while pH stability was in the
Five Saccharomyces cerevisiae isolated from the ability of chitinase production from the isolates were studied. Quantitative screening appeared that Saccharomyces cerevisiae S4 was the highest chitinase producer specific activity 1.9 unit/mg protein. The yeast was culture in liquid and solid state fermentation media (SSF). Different plant obstanases were used for (SSF) with the chitine, while liquid media contained chitine with the diffrented nitrogen source. The favorable condition for chitinase producers were incubated at 30 ºC at pH 6 and 1% colloidal chitine.
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