Genetic variation was studied in 22 local and imported samples collected from local Iraqi market by using random amplified polymorphic DNA (RAPD-PCR). Five randomly primers set were used in this study. These primers produced 292 bands. Molecular weights of these bands ranged between 1.8 Kb (1800 bp) to 150 bp. The percentage of polymorphic bands is 100%, with one distinguished band which is produced by using C52 primer. The other primers did not produce any distinguished band. The results of Dendrogram of the studied samples depended on RAPD-PCR results by using Jaccard coefficient for genetic similarity was distributed the samples into 8 groups. This Dendrogram revealed a higher similarity between Iraqi/Yousifia green bell pepper and Jordanian yellow bell pepper with 0.84 values. This value is the highest between samples in comparison with lowest values (0.0) which are found between Iranian yellow bell pepper and the other samples. There are another high values were revealed between Italian orange and red bell pepper with 0.74 values. Also, other high similarity values revealed between Spanish red bell pepper and Italian green bell pepper with 0.73 values; Iranian red bell pepper and Jordanian green bell pepper with 0.72 values; Iraqi/Souwyera green bell pepper and Iraqi/Yousifia green bell pepper with Jordanian yellow bell pepper (the last two samples in same subgroup) with 0.69 values, and last between Iraqi/Balad green bell pepper and Chinese red bell pepper with 0.61 values. The genetic distance between studied samples by using Euclidean coefficient revealed the highest values between Chinese yellow bell pepper and Iranian green bell pepper with 5.0 values, in comparison with the lowest genetic distance between Spanish green bell pepper and Italian yellow bell pepper with 1.0 value.
Twenty four bacterial isolates were identified from (10) places for wandering sellers in south Baghdad city (Bayaa garage). They were Staphylococcus aureus (9 isolates), Bacillus subtilis (6 isolates), Salmonella spp. (4 isolates) and Psudomonas aeruginosa (5 isolates). Agar well diffusion method was used to definition sensitivity of the fresh and dried juice of Capsicum grossum L. and Allium cepal L. at different concentrations. The fresh juice had no inhibitory activity against the bacterial isolates in contrast to the fresh juice , dried juice which show marked activity against all bacterial isolates at (30) mg/ml.
Chemical Methodologies (CHEMM)
(1)
An experiment was carried out in the vegetables field of Horticulture Department / College of Agriculture / Baghdad University , for the three seasons : spring and Autumn of 2005 , and spring of 2007 , to study the type of gene action in some traits of vegetative and flowery growth in summer squash crosses (4 x 3 = cross 1 , 3 x 7 = cross 2 , 3 x 4 = cross 3 , 3 x 5 = cross 4 , 5 x 1 = cross 5 , 5 x 2 = cross 6). The study followed generation mean analysis method which included to each cross (P1 , P2 , F1 , F2 , Bc1P1 , Bc1P2) , and those populations obtained by hybridization during the first and second seasons. Experimental comparison was performed in the second (Two crosses only) and third seasons , (four crosses) by using RCBD with three
... Show MoreThe identification of salinity-tolerance genes is a critical aspect of the new molecular technology. In this work cDNA-RAPD is used for the identification of genes expressed in salt tolerant but not in salt sensitive wheat. Two cultivars wheat, salt tolerance (Dijla) and sensitive (Tamooz2) were used for the preparation of RNA and cDNA synthesis. Eight primers were used for random amplification of cDNA constructed from RNA and three primers were differentially expressed in salt tolerant cultivars. Genes related to salt tolerant were predicted using NCBI blast for the three primers. The predicted genes were involved in salt tolerance of wheat and other plants as well. This indicates the suitability of the primers and the method for
... Show MoreFood fortification has an important and necessary role in compensating for the shortage of nutritional micronutrients, especially in developing and least developed countries. So, 12 samples of flour available in the local market, whether imported or locally produced flour, were obtained during 2019. The amount of base metal of the necessary iron element in the flour models studied which are available in local markets, measured by spot testing and was compared with the values that should be added according to the specification Iraqi standard. Results revealed the qualitative evaluation of iron in locally produced flour does not conform to the Iraqi standard and is almost free of any reinforcement. While the percentage of imp
... Show MoreGenetic polymorphism in a fragment of NADH (ND4),400bp long from the Mitochondrial DNA
(mtDNA) of Mediterranean fruit fly ceratitis capitata (Wiedemann, 1824) using PCR-RFLP method
with the restriction enzyme EcoRV in samples collected from three governorates in the middle of Iraq.
The purposes of this study is to establish database, discover the introduction source as well as studying
the genetic diversity for this economic pest in Iraq. The results show that there is a genetic
polymorphism of the studied gene fragment among Kut governorate as compaired with the other
studied samples according to digestion results of the restriction enzyme EcoRV.
An experiment was carried out in the vegetables field of Horticulture Department / College of Agriculture / Baghdad University , for the three seasons : spring and Autumn of 2005 , and spring of 2007 , to study the type of gene action in some traits of yield and its components in summer squash crosses (4 x 3 = cross 1 , 3 x 7 = cross 2 , 3 x 4 = cross 3 , 3 x 5 = cross 4 , 5 x 1 = cross 5 , 5 x 2 = cross 6). The study followed generation mean analysis method which included to each cross (P1 , P2 , F1 , F2 , Bc1P1 , Bc1P2) , and those populations obtained by hybridization during the first and second seasons. Experimental comparison was performed in the second (Two crosses only) and third seasons , (four crosses) by using RCBD with three repl
... Show MoreThe aim of this study was the discrimination of Salmonella isolated from chicken and their feed and drinking water for the epidemiological control of salmonellosis. Totally, 289 samples, including 217 chicken cloaca swabs, 46 water, and 26 feed samples were collected from five different farms in Karbala governorate, Iraq. Conventional bacteriology tests, API 20E, Vitek 2, and serology were used for bacterial identification. Random amplified polymorphic DNA (RAPD)-polymerase chain reaction (PCR) was applied to analyze the genetic relationships among Salmonella isolates. The isolation rate of Salmonella spp. was 21.1% (61/289). While the water samples constituted the highest rate (30.4%), a rate of
... Show More
(2)
