A simple, sensitive, accurate and economic spectrophotometric method has been developed for the determination of sulfacetamide (SFA) in pure form, synthetic sample and urine. The method is based on diazotization of primary amine group of sulfacetamide with sodium nitrite and hydrochloric acid followed by coupling with chromotropic acid in alkaline medium to obtain a stable orange colored chromogen which exhibit a maximum absorption (λmax) at 511.5 nm. Different variables affecting the completion of reaction have been carefully optimized following the classical univariate sequence and modified simplex method (MSM). Under optimized conditions, Beer’s law obeyed in the concentration range of (0.5- 20.0 µg.ml-1) with molar absorptivity of 3.2186*104 L.mol-1.cm-1. The limit of detection was 0.054 µg.mL-1 and Sandell’s sensitivity value 7.8989 µg.cm-2. The proposed method was successfully applied to the determination of (SFA) in synthetic sample and urine.
S rum total. siic acid (TSI\) ·and lipid bound sialic acid {LSA) levels were detennined by spec.tr0photoinetric me(hods in 23 patients with oral cavity cancer(OCC). Of them 5 patients· have carcinoma ·of the anterior 2/3 of the tongue with an avetage age of { 60±8:0 year:;;}' and 2 males patients with carcinoma of the inner surface of the cheek with an average age {68±8.0 years·} and 8 patients had carcinomtl of rhflo"Qr of the mouth ,y-tt.h an av.erage {'62±9.0 ye.ars} a11d 8 patients with carcii1otna of the hard palate with an average age {58±8.0 years}. They were compated witn 32 healthy controls; &
... Show MoreA spectrophotometric method is proposed for the determination of some drugs containing amino group such as mesalazine, metoclopramide and dopamine in pharmaceutical formulations. It was simple, precise, accurate, rapid, and based on the oxidation of each drug with chromate as an oxidizing agent in the presence of 1N hydrochloric acid. Then indigo carmine is reacted with residual chromate in the presence of a catalysis factor (sodium oxalate). Increasing in absorbance's value of the color system is proportional to the amount of the three drugs which is measured at the selected wavelength of 610 nm.
The proposed method is obeying Beer's law in the ranges of (1-40, 2-44 and 2-52) ppm for the concentration of
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Background: The most crucial mechanism of genetic variation in N. meningitidis is the slipped strand mispairing, this mechanism generates Phase variation using simple sequence repeat (SSR) and is commonly used by the N. meningitidis to escape the immune system despite its function in eradicating the pathogenic and commensal bacteria. Some of simple sequence repeats (SSRs) that located within the genome works as phase variation while other SSRs have no role in generating phase variation mechanisms. Therefore, Aim: the main goal of the current in silico study was to detect the probability of SSR to enroll with phase variation for the entire N. meningitidis genome. Methods: Different criteria were used to judge SSR as
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The aim of this study is to assess the influence of some risks factors on the fistula development after palatoplasty to improve the outcome of the patients
A total of 48 patients (the males were 22, The females were 26) were included in this study. All the patients were examined weekly for the first month postoperatively to assess any breakdown in the wound by inspection and by asking the parents for any nasal regurgitation during fluids feeding.
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The research includes attempts to prepare anthranil ic acid (C7H7N02) complexes with some metals: [Pd (II), Fe(ll), and Fe (Ill)) which have been characterized by using:
Thermal stability (melti ng point, d composition poit), molar conductivity, IR, UV-visible spectra, elemental analysis (C-H-N) and magnetic properties. The general formula has been given for the prepared complexes:-
- M(C2H6N02)2 Where M= [Pd(ll), Fe(TI), Hg(ll)]
:.. M(C1H6N02)l Where M= [Fe(llf)]
A sensitive and environmentally benign spectrometric method was developed for quantifying Meprobamate (MEP). The analyzed MEP was derivatized into a colored complex and determined spectrometrically. The colorimetric analytical parameters were optimized and validated. Low limit of detection (LOD) was achieved down to 1.88×10-6 mol/l while the limit of quantification (LOQ) was extended over the range of 1.97×10-6 - 1.35×10-3 mol/l. The high precision has been denoted by the 1.54% value of the coefficient of variation. The recovery was 96.07%, while the RSD (n=3) was 1.05 - 1.19%. The apparent molar absorptivity (Æ) obtained within 1154.7 - 1691.9 L.mol-1.cm
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Simple and sensitive kinetic methods are developed for the determination of Paracetamol in pure form and in pharmaceutical preparations. The methods are based on direct reaction (oxidative-coupling reaction) of Paracetamol with o-cresol in the presence of sodium periodate in alkaline medium, to form an intense blue-water-soluble dye that is stable at room temperature, and was followed spectrophotometriclly at λmax= 612 nm. The reaction was studied kinetically by Initial rate and fixed time (at 25 minutes) methods, and the optimization of conditions were fixed. The calibration graphs for drug determination were linear in the concentration ranges (1-7 μg.ml-1) for the initial rate and (1-10 μg.ml-1) for the fixed time methods at 25 min.
... Show MoreRapid, reproducible and accurate method has been developed for the assay for of mebendazol (MBZ) residual assay. The method is based on alkaline hydrolysis of MBZ with sodium hydroxide then oxidation with N-bromosuccinimide (NBS) followed by coupling with 4-Bromoaniline (4-BA) to yield a highly colored product absorbed at maximum 434 nm. Regression analysis of linearity range was found (0.6-2.8) µg.ml-1. The optimum conditions that affect the oxidation were studied. The developed method was found to be precise with mean value of relative standard deviation (1.153- 1.303) and accurate with relative error (-0.5940-1.7821) .The calculated molar absorptivity and sandal sensitivity values of (29825 L.mol-1.cm-1), 0.0099 µg.cm-2 respe
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The complexes of para-chloranil as electron acceptor and the anions of amide, azide and cyanide as electron donors in aqueous ethanol as a solvent, were studied spectrophotometrically . The reactions lead to the formation of charge transfer complexes. The CT complexes were stable in excess acceptor concentration, while they were underwent another transformations in excess donors concentrations. Stoichiometries were determined, the molecular ratio was determined by continuous variation method (Job method) and is was 1:1 (donor: acceptor). The maximum wavelength (λ max.), the energy (hυCT), ionization potential (Ip) and activation energy (w ) of excited state f
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