Preferred Language
Articles
/
jih-3254
Isolation and Identification of Endophytic Fungi from Aloe vera Leaves and Chemical Analysis of the Alcoholic Extract of the Leaves Using HPLC, GC, and GC-Mass Devices

The study's goals were to separate and identify endophytic fungi from Aloe vera leaves by looking at their morphology and molecules, as well as to find the chemical compounds in the leaf extract by using HPLC, GC, and GC-Mass instruments. The results showed that 53 endophytic fungi were isolated from a total of 120 pieces of A. vera leaves, with a total colonization rate of 44.16%. The fungus Aspergillus terreus had a colonization rate of 14.16%; Aspergillus niger had a colonization rate of 13.33%; Penicillium chermesinum demonstrated a colonization rate of 6.66%; Paecilomyces variotii had a colonization rate of 2.5%; Talaromyces radicus; and Aspergillus flavus achieved a colonization rate of 1.66%. Finally, the fungi Aspergillus quadrilineatus, Talaromyces verruculosus, Neoscytalidium dimidiatum, Alternaria solani, and Aspergillus niveus achieved a colonization rate of 0.83%. The results of examining the alcoholic extract of the leaves using the HPLC device showed the presence of the chemical compounds aloin at a concentration of 125.39 ppm and aloe emodin at a concentration of 66.59 ppm. We looked at the leaf alcoholic extract with a GC machine and found a group of fatty acids. These included linoleic, oleic, palmitic, and stearic. The GC-MS test revealed a group of active compounds, including Heptane, 1-(ethenylthio), Ethanedicarboxamide, N-allyl-N'-(2,5-dimethylphenyl), 2H-Pyran, 2-(3-butynyloxy) tetrahydro, 1,2-Cyclobutanedicarboxylic acid, 3-methyl-dimethyl ester and 4 (1H)-Pyrimidinone, 2-(propylthio). The presence of endophytic fungi from which effective enzymes or compounds can be isolated could probably have an important role in future medical and therapeutic uses. Also, the leaves of the A. vera plant have medicinal and therapeutic uses for many diseases.

Crossref
View Publication
Publication Date
Sun Mar 01 2020
Journal Name
Indian Journal Of Forensic Medicine & Toxicology
Determination of Some Volatile Organic Compounds in the water produced at Al-Ahdab oilfield in the Governorate of Wasit, Iraq using Headspace SPE-GC-FID

The study was conducted over the period of Oct 2018 to Apr 2019 and is aimed for the detection and estimation of four hazardous Volatile Organic Compounds VOC (benzene, toluene, ethylbenzene, and xylene) so-called (BTEX) in samples collected from the produced water in the Al-Ahdab oil field in Iraq also to track their availability in the important natural water sources around the field. These compounds pose a risk to human health as well as environment. To avoid the laborious and tiresome conventional extraction methods, water samples were collected and concentrated using solid-phase extraction technique (SPE) which is a robust and cost-effective method of sample extraction with minimal exposure and handling of solvents and then to be analy

... Show More
Preview PDF
Scopus (1)
Scopus
Publication Date
Mon Jan 01 2018
Journal Name
Acta Pharmaceutica Sciencia
Phyto-fabrication, characteristics and anticandidal effects of silver nanoparticles from leaves of ziziphus mauritiana lam

In this work, silver nanoparticles (AgNPs) were biosynthesized from leaves of Ziziphus mauritiana Lam. jujube plant in Iraq and tested against fungal pathogens. Extract of leaves of Z. mauritiana mixed with 10-3 M AgNO3exposed to slight sunlight for 3 days. Characterization of AgNPs was done using UV-visible spectroscopy, SPM (scanning probe microscopy) and atomic force microscopy (AFM). The change of solution color from pale brown to dark brown and the exhibited maximum peak at 445 nm accepted as an indicator to biosynthesized AgNPs. Aqueous extract of Ziziphus mauritiana is considered as biological reduced and stabilized agent for Ag+ to Ag0. AFM showed the formation of irregular shapes of AgNPs. The biosynthesized silver nanoparticles ha

... Show More
View Publication
Scopus (29)
Crossref (7)
Scopus Crossref
Publication Date
Sun Mar 05 2017
Journal Name
Baghdad Science Journal
Study the Effects of Olive Leaves Extracts in the activity of the enzyme GOT and their Biological Activities

The olive tree, has been used it is important plant for the time being some of their parts on a large scale in the treatment of gastrointestinal disorders and stimulate circulation . Moreover, it is used as antibacterial material and also to address some of the respiratory system, diabetes, food preservation osteoporosis. This study involved the collection of olive leaves from different areas in Baghdad / Iraq. These leaves have been harvested, wash it, then dried and crushed, where the study aimed to identify the active ingredients and chemical elements in the olive leaf as well as its effect on the action of GOT enzyme .The study showed that the aqueous extracts (cold and hot) of the olive leaves powder are acidic in nature pH values

... Show More
View Publication Preview PDF
Scopus Crossref
Publication Date
Thu Apr 28 2022
Journal Name
Iraqi Journal Of Science
A comparative study between the effect s of Arctium lappa L. leaves extract and Pentoxifylline on DNA of sperms rats treated with Gentamicin

This study were designed to make comparison between the influence alcoholic extract of Arctium lappa L. leaves and Pentoxifylline on treatment the adverse effect of gentamicin on DNA of sperms. Thirty five male rats were divided into 5 groups: the 1st group (T1) was made as negative control group, the 2nd group (T2) was given distilled water and gentamicin 5mg/kg (positive control group), the 3rd group (T3) was given a dose 600 mg/kg of Arctium lappa L and gentamicin, the 4th group (T4) was given dose 100mg/kg of pentoxifylline and gentamicin, the 5th group (T5) was given a dose 300mg/kg of Arctium lappa extract with dose 50mg/kg of pentoxifylline and gentamicin. The results of DNA damage% and agglutination % in T3, T4 and T5 appeared an

... Show More
View Publication Preview PDF
Publication Date
Tue Jul 30 2019
Journal Name
Sn Applied Sciences
Removal of oil emulsion from aqueous solution by using Ricinus communis leaves as adsorbent

View Publication
Scopus (9)
Crossref (10)
Scopus Clarivate Crossref
Publication Date
Fri Jan 15 2021
Journal Name
Plant Archives
ISOLATION AND IDENTIFICATION OF GBS BACTERIA FROM MASTITIS BY CAMP TEST AND LANCEFIELD’S SEROLOGICAL GROUPING

Mastitis is an udder tissue inflammation which has infected various species of animals. It happens through several types of pathogenic bacteria, particularly Streptococcus agalactiae. GBS is a leading cause of cow mastitis. In our sample, 9.52% of Streptococcus agalactiae were isolated which were collected from bovine mastic milk and identified by biochemical tests such as catalase, oxidase, Production of indole, fermentation of sugar, an examination of antibiotic sensitivity, CAMP test and group kits of Lancefield. The results showed that all Streptococcus agalactiae isolate was diagnosed by CAMP test by the appearance of the arrowhead in blood agar and by the appearance of visible agglutination on a card in the serological grouping kit of

... Show More
View Publication Preview PDF
Crossref
Publication Date
Wed Jun 08 2022
Journal Name
International Journal Of Health Sciences
Isolation and identification of Aspergillus fumigatus from feline respiratory infection in Baghdad province

Aspergillus fumigatus considered to be the most important species to cause respiratory infection cases in both humans and animals especially in cats in the last decades. In this study, we focused on the isolation and identification of Aspergillus fumigates by collecting 40 samples in deferent veterinary clinics and stray cats in Baghdad city, during the period (October 2021 to January 2022), all samples were cultured on Sabouraud dextrose agar and malt extract agar. The isolates identified by the laboratory methods, it’s depend on macroscopic and microscopic appearance. The results showed that (40) swaps taken from the pharynx of infected cats, included: Aspergillus fumigatus 16 (40%), Aspergillus spp. 7 (17.5%), Aspergillus niger

... Show More
View Publication
Crossref
Publication Date
Sat Dec 30 2023
Journal Name
Iraqi Journal Of Pharmaceutical Sciences( P-issn 1683 - 3597 E-issn 2521 - 3512)
Isolation, Identification, and Quantification of Two Compounds from Cassia glauca Cultivated in Iraq

The Cassia glauca Lam. is the tree that belongs to the Fabaceae family and is native to India has many uses in indigenous systems of medicine, folk medicine, and traditional Brazilian medicine. Has many pharmacological activities such as anti-diabetic, antibacterial, antifungal, antioxidant, anti-hemolytic, anticancer, cardio-protective, and Hepato-protection.  The aim of study is to Isolation, identification, and quantification of some compounds from aerial parts of Cassia glauca since no phytochemical investigation had previously been done in Iraq for this plant. The aerial parts were defatted in n. hexane for 48 hours. The defatted materials were extracted in 85% ethanol using the hot method (soxhlet), then the extract was fra

... Show More
Scopus (1)
Scopus Crossref
Publication Date
Sun Jan 01 2023
Journal Name
Brazilian Dental Science
Wound healing effect of a one-week Aloe Vera mouthwash: a pilot study

View Publication
Scopus (1)
Scopus Crossref
Publication Date
Fri Mar 01 2024
Journal Name
Baghdad Science Journal
Impact of alkaloids extract of Moringa olievera Lam. leaves on the development, fertility and demography of the southern cowpea beetle insect Callosobruchus maculatus (F.) (Coleoptera: Bruchidae)

The Southern Cowpea Beetle Callosobruchus maculatus (F.) is one of the most widespread insect pests of stored legumes, causing a considerable loss during storage, decreasing the net weight of the crops, and resulting in reduced the quality of the crops. This study has been conducted to determine the lifetime, fertility and life table parameters of C. maculatus by using an alkaloids extract from Moringa oleifera leaves at different concentrations 1000, 2000, and 3000 ppm. The result was shown that the lowest survival rate was 49% at a concentration of 1000, 2000 ppm, as compared with the control which was 77%. The lowest reproductive rate (Ro) was 4.76 female/female/generation at the concentration of 1000 ppm, c

... Show More
View Publication Preview PDF
Crossref (1)
Scopus Crossref