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Lactic Acid Bacteria Biosurfactant Role That Isolated from Human Breast Milk in Inhibit Eyes Pathogenic Bacteria
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     Biosurfactants have a wide-range of applications due to their unique properties like specificity, not toxicity (from LAB) and relative ease of preparation. These properties hold promise of biosurfactants to increase breast milk benefit were isolated and described into Lactobacillus plantarum, Lactobacillus fermentum ,Lactococcuslactis, and Leuconostocmesenteroides.The degree of microbial destruction of disease, which promotes  the effective remediation of disease spreading.This paper presents a review of available research, methods and publications regarding Biosurfactant extraction from Lactic Acid bacteria isolated from human breast milk. 3 samples of human breast milk was provided, LAB were isolated and described, Biosurfactants recovery and surface activity were tested and extracted endo and extra cellular. In other side 26 samples from eye patients were ordered, diagnosed and their sensitivity to biosurfactant were studied. The results showed that 5 isolates of LAB from human breast milk were biosurfactant producer but L. plantarum was the more efficiency in surface activity. In other side, out of 26 eyes sample 18 were positive to pathogenic bacteria included E.coli (7), Klebseilla (5), Staphylococcus aureus(3)and S.epidermides (3).Extracellular Biosurfactanthad good effect against tested bacteria, but endocellular (extraction by normal method) had not any effect against any bacteria, whereas by solvents method were the more active against all tested bacteria. The results are promising enough to continue the quest for enhancement of inhibition growth of pathogenic bacteria with biosurfactant application (extracted extracellular by solvents) to look forward for biosurfactant as a solution of antibiotic resistance problem. In this study we concluded thatL. plantarum was the more effectiveness in biosurfactant surface activity and the extracellular biosurfactant by solvent method for extraction were better than endocellular and normal method of extraction.

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Publication Date
Sun Dec 01 2013
Journal Name
Baghdad Science Journal
Bacterial Causes Tonsillitis in Children, Study the Resistance to Antimicrobials and the Effect of Clove Extracts on Selected Isolated Bacteria.
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In this study Isolated Pathogenic bacteria which causes Tonsillitis in Children with ages between 3-17 years. They are admitted to Central Children Hospital (Al-Karch) and Ebn-Albalady Hospital (Al-Rusafa). 200 cases were collected which include 120 Male and 80 Female. The result of the recent study shows that the isolation percentage was 40% from Male and 35% from Female. In this study Fifty six isolated were Identified, 20 were ?-hemolytic Streptococcus which was Streptococcus pyogenes, formed (36%) from all isolated.6 Pathogenic bacteria were ?- hemolytic Streptococcus which was Streptococcus pneumoniae formed (11%). The number of Moraxella catarrhalis bacteria was 12 formed (21%), the number of Haemophilus influenzae was 1

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Publication Date
Wed Jun 28 2023
Journal Name
The Iraqi Journal Of Veterinary Medicine
Antibacterial Activity of Lactiplantibacillus plantarum‎ from Dairy Products ‎Against Some ‎Foodborne Bacteria
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Lactiplantibacillus plantarum‎, one of lactic acid bacteria (LAB), is found in various foods, ‎‎including dairy products, meat, and vegetables, and most of these bacteria offer beneficial ‎‎effects to humans and animals as potential probiotics with broad-spectrum antimicrobial ‎‎activities. The aim of this study was evaluating the antibacterial efficacy of L. ‎plantarum‎ ‎against ‎some foodborne bacteria isolated from dairy products. This research involved 34 dairy ‎products, ‎including local and imported milk, cheese, and yogurt sold locally in Baghdad ‎province, Iraq, during May ‎‎2022. For the isolation of L. ‎plantarum‎, a special medium called ‎MRS (de Man Rogosa and ‎Sharpe) was applied. Colonie

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Publication Date
Sun Sep 04 2016
Journal Name
Baghdad Science Journal
Bioadsorption of Heavy Metals From Industrial Wastewater Using Some Species of Bacteria
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Three isolated bacteria were examined to remove heavy metals from the industrial wastewater of the Diala State Company of Electrical Industries, Diyala-Iraq. The isolated bacteria were identified as Pseudomonas aeruginosa, Escherichia coli and Sulfate Reducing Bacteria (SRB). The three isolates were used as an adsorption factor for different concentrations of Lead and Copper (100, 150, and 200 ppm.), in order to examine the adsorption efficiency of these isolates. In addition, the effect of three factors on heavy metals adsorption were examined; temperature (25, 30, and 37 ?C), pH (3 and 4.5) and contact time (2 and 24 hrs). The results showed that the highest level of lead adsorption was obtained at 37 ?C by E. coli, P, aerugenosa and

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Publication Date
Mon Jul 01 2019
Journal Name
Journal Of Physics: Conference Series
Production and antibacterial activity of biosurfactant from Saccharomyces cerevisiae
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Abstract<p>A total of four types of instant dry yeast <italic>Saccharomyces cerevisiae</italic> which are Gloripan, Swan, Super Man (China) and Saf (Turkey), were obtained from the local market in Baghdad, Iraq. All the types were identified according to their morphological characteristics and screened for their ability to produce biosurfactant by oil spread method. Biosurfactant was extracted and partially purified by precipitation with ammonium sulfate at saturation ratio75%. Antibacterial activity of crude and partially purified biosurfactant was tested against twenty isolates of <italic>Pseudomonas aeruginosa</italic> and the results showed that crude biosurfactant has no</p> ... Show More
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Publication Date
Wed May 10 2023
Journal Name
Biomass Conversion And Biorefinery
Lactic acid-based deep eutectic solvents and activated carbon for soap removal from crude biodiesel
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Publication Date
Fri Mar 10 2023
Journal Name
Proceedings Of The Pakistan Academy Of Sciences: B. Life And Environmental Sciences
Characterization of Titanium dioxide (TiO2 ) Nanoparticles Biosynthesized using Leuconostoc spp. Isolated from Cow’s Raw Milk
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Nanotechnology is a continually expanding field for its uses and applications in multiple areas i.e. medicine, science, and engineering. Biosynthesis is straightforward, less-toxicity, and cost-effective technology. TiO2 NPs biosynthesis has attained consideration in recent decades. In this study, probiotic bacteria were isolated from cow’s raw milk samples, and then were identified by using the Vitek2 system; as Leuconostoc spp. included Leuconostoc mesenteroides subsp. mesenteroides (Leu.1), Leuconostoc mesenteroides subsp. cremoris (Leu.4), and Leuconostoc pseudomesenteroides (Leu.14). All Leuconostoc spp. isolates showed an ability for TiO2 NPs bio-production, after being incubated at anaerobic conditions (30 o C/ 24 h) in DeM

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Publication Date
Thu Jul 05 2018
Journal Name
International Journal Of Medical Research & Health Sciences
Methicillin-Resistant Staphylococcus epidermidis Isolated from Breast Tumors of Iraqi Patients
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Background: Globally, breast cancer is the second leading cause of death among women in Iraq. Several genetic and environmental factors are associated..

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Publication Date
Thu Apr 25 2019
Journal Name
Iraqi Journal Of Market Research And Consumer Protection
STUDY OF THE SYNERGISTIC EFFECT OF BACTERIOCIN PRODUCED FROM Leuconostoc mesenteroides ssp. cremoris With Antibiotics Against Multi Resistance Diarrheal Bacteria: STUDY OF THE SYNERGISTIC EFFECT OF BACTERIOCIN PRODUCED FROM Leuconostoc mesenteroides ssp. cremoris With Antibiotics Against Multi Resistance Diarrheal Bacteria
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The study included the collection of samples of raw cow milk to isolate Leuconostoc bacteria, samples were sub cultured on De-Man Rogosa Sharpe-Vancomycin medium, the pure colonies were selected and subjected to the cultural and microscopically tests, according to that 25 cocci bacterial isolates were obtained, then isolates were subjected to biochemical tests. Result of tests showed that 12 isolates belong to the genus Leuconostoc out of 25 cocci bacterial isolates, Vitek2 system was used as a supplementary step. Results of final identification showed that 3 sub species were obtained included Leuconostoc mesenteroides ssp. cremoris 9 out of 12 isolates, while it was 2 isolates of Leuconostoc mesenteroides ssp. mesenteroides and one isol

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Publication Date
Tue Jun 30 2020
Journal Name
Iraqi Journal Of Market Research And Consumer Protection
EFFICIENCY EVALUATION OF ALKALOIDS FROM POMEGRANATE (Punica granatum) PEELS TOWARDS SOME PATHOGENIC FUNGI OF HUMAN: EFFICIENCY EVALUATION OF ALKALOIDS FROM POMEGRANATE (Punica granatum) PEELS TOWARDS SOME PATHOGENIC FUNGI OF HUMAN
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This study focused on the role and importance of alkaloid compounds in Punica granatum peels which is one of many wide distribution medicinal fruits. Two kinds of pathogenic fungi were isolated from patients in Baghdad to be tested, also a type of extracts was prepared, alkaloids were isolated and partially purified and detected by two ways, a classic depended technique also used for determine these alkaloids, results showed an observed differences among extracts or treatments towards the fungi samples. So this study was one of the scientific applications to find natural alternative compounds that inhibit the growth of several pathogenic organisms that cause dangers and harms for human health.

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Publication Date
Tue Sep 01 2020
Journal Name
Gene Reports
Efflux pumps of Gram-negative bacteria in brief
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he development of multidrug resistance in Gram-negative bacteria is a major problem faced antimicrobial therapy and management of infectious diseases. Too many resistance mechanisms were developed since the antimicrobial agents were produced and actually used. The mechanisms involved in antimicrobial agents' resistance are, modifying enzymes, alteration of the target site of antimicrobials and prevention of antimicrobials accumulation inside the bacterial cells. The latest is accomplished by two mechanisms: alteration of outer membrane permeability and efflux pumps. Efflux pumps are either chromosomal or plasmid-encoded although chromosomal encoded efflux pumps are common in Gram-negative bacteria. Resistance nodulation division (RND) efflu

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