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Sensitivity of Bacteria Escherichia coli Iisolated from Urinary Tract Infection toward Ciprofloxacin and Lomefloxacin.
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   Four hundred and fifty urine samples were collected from patients suffering from urinary tract infection from the General Azadi hospital in Kurkuk province ,during the period of october 2007 till march 2008 .       Results of bacteriological culture revealed that (168) out of (450) studied samples (37.3%) gave positive culture using blood agar and macConkey agar ; different species of bacterial isolates were detected using morphological and biochemical tests ,from these isolates the highest percentage of the isolates were from Escherichia coli when it was (100) isolates out of (190) isolate  (52.63%)  .  one hundred isolates were distributed between (77) from females and (23) from males     The susceptibility  of E.coli isolates towards (13) types of antimicrobial agents commomly used in treatment of urinary tract infection were tested in vitro . Variations in susceptibility against antimicrobial were found. The antiseptic drug Nitrofurantoin showed the highest activity on E.coli when the resistance reached  (14 %) , from the other hand E.coli isolates showed great resistance to other antimicrobial : sulphamethoxazole was (100 %) ,Ampicillin (99 %) ,Cephalexin   (62 %) ,Agumentin (58 %) , Nalidixic acid (54 %) , Trimethoprim (52 %) , Pipracillin (45 %) , Ceftriaxone (40 %) , Lomefloxacin (39 %) , Cefotaxime (39 %) , Ciprofloxacin (37 %) and to Co-trimxazole was (37 %) .     Minimal inhibitory concentration (MIC) for Ciprofloxacin and Lomefloxacin were studied and the results showed higher values in MIC. MIC values for eight  isolates of bacteria  E.coli were so high they were (400> µg/ml) for Ciprofloxacin were (19.5%) while five isolates of bacteria E.coli were with  MIC value of (400>µg/ml) with (11.6%) to Lomefloxacin .        The ability of bacteria E.coli isolates  to produce ß-lactamase enzyme using Capillary tubes method was studied. The results showed (61 %) of these isolates gave positive results when the red cloer of the indicator phenol was changed from red to yellow within (15) minuets .When using Disc approximation method to detect the ability to production Extended Spectrum ßlactamase enzyme (ESBLs) out of (61)isolates only (11)isolates were positive (18 %); Nine ESBLs producer showed high resistance to antimicrobial Quinolones (Naldixic acid ,Ciprofloxacin and Lomefloxacin) with percentage reached (72.7%) . 

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Publication Date
Thu Jan 13 2022
Journal Name
Iraqi Journal Of Science
A Comparative Study of Amoxicillin Sensitivity Against Escherichia coli Isolates Isolated from Urinary Tract Infections
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The purpose of this study was to investigate the bacterial etiology of urinary tract infections microbiologic properties of Escherichia coli isolated from urinary tract infection patients against nine amoxicillin antibiotic. E.coli isolates were collected from patients samples suffering from urinary tract infection, based on biochemical tests of Epi 20 system .Nine Amoxicillin antibiotics were selected (some vials and other are capsules) which manufactured in different countries were bought from local pharmacies in Baghdad, for the purpose of knowing the inhibitory activity of these antibiotics on E.coli one of the main microorganisms to cause urinary tract infection, the antibiotics were prepared in a concentration of 100mg/ml and their

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Publication Date
Wed Dec 01 2021
Journal Name
Gene Reports
The molecular study for evaluation the antibiotic resistance of Escherichia coli and Klebsiella pneumoniae bacteria isolated from urinary tract infection patients
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Urinary tract infection is a bacterial infection that often affects the bladder and thus the urinary system. E. coli is one of the leading uropathogenic bacteria that cause urinary tract infections. Uropathogenic E. coli is highly effective and successful in causing urinary tract infections through biofilm formation and urothelial cell invasion mechanisms. Other organisms that cause urinary tract infections include members of the Enterobacteriaceae family, streptococci and staphylococci species and perch. In addition, K.penumoniae is another important gram-negative bacterium that causes urinary tract infections. With the PCR technique, unseen bacterial species can be detected using standard clinical microbiology methods. In this study, the

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Publication Date
Thu Nov 30 2023
Journal Name
Iraqi Journal Of Science
Biodisintegration of human mucin protein by protease produced from Escherichia coli AJ55 isolated from Urinary Tract Infection of Iraqi pa-tients
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      Proteases have various applications in the food, pharmaceutical, medicine, pathogenicity of some pathogenic bacteria, and detergent sectors as well as meeting the needs of approximately 60% of the global enzyme industry, whereas they catalyze the breakdown of protein molecules into peptides and amino acids. Production and purification of protease enzyme by the isolate Escherichia coli AJ55 was scrutinized in the present study. Cultivation optimum conditions, were various complex medium, carbon source, nitrogen source, temperature, pH of the medium, and time of incubation were optimized to enhance the total protease production in shake flask culture of E.coli AJ55. The nutrient broth supplemented with 2% gluco

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Publication Date
Thu Jun 30 2016
Journal Name
Al-kindy College Medical Journal
Detection similarity and differences between Uropathogenic Escherichia coli isolated from recurrent urinary tract infections and bladder cancer patients
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Background: Urinary tract infections (UTIs) and their complications such as Bladder cancer (Bl. C.) are a health growing problem worldwide. Objective: To shed light on this subject, present study was done to investigate relationship between recurrent urinary tract infection (RUTI) due to Escherichia coli (E. coli) and Bl. C.Type of study: Cross-sectional study. Methods: This study included 130 patients with RUTI, 50 patients with Bl. C. and 50 control of both sexes (aged 7-85 years) attending Al-Zahra Teaching Hospital in Al-Kut/Wassit governorate and Al-Harery Teaching Hospital of specialized surgeries/Baghdad. The patients were divided into two groups: the first group (n=130) included those who were suffering from recurrent UTI without

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Publication Date
Sun Apr 23 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Detection of Uropathogens in Urinary Tract Infection and Their Sensitivity to Many Antimicrobial Agents
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    Urinary tract infections (UTI) are some of the most common infections experienced by humans, exceeded in frequency among ambulatory patient only by respiratory and gastrointestinal infections. It is also the most common cause of nosocomial infection in adults.   A total of three hundred urine sample were collected in age (1-69 years old)   in both gender, with (UTI) symptoms referred to AL-Yarmok Teaching Hospital at Baghdad city during the period from January 2010 till August 2010.  The commonest isolates were Escherichia coli (E.Coli), Proteus mirabilis and Klebsiella pneumoniae (These represented 49.2%, 22 %, and16 % of isolates respectively). The percentage of bacteria incidence in females

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Publication Date
Thu Nov 21 2019
Journal Name
Al-kindy College Medical Journal
Effect Of Pomegrante Peels And Bay Leaves On Multidrug Resistant Bacteria Isolated From Urinary Tract Infection Patients
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Background: Alternative natural therapy by plants extracts had opened  wide  door   for   the    use  of  natural products as an alternative therapy instead of many antibiotics and drugs , which had many harmful side effects.Also, an increased interest has been centered on the industrial wastes, especially plant raw materials which contain phenols (e.g. Pomegranate peel and Bay leaves) which is a sources of natural antioxidants ,which are on the contrary  of  synthetic  antioxidants that  had restrict use  due to  their  health  risks , carcinogenesis and toxicity .

Objectives :This study was done to fi

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Publication Date
Tue Feb 01 2022
Journal Name
Baghdad Science Journal
An Epidemiological and Multidrug Resistance Study for E. coli Isolated from Urinary Tract Infection (Three Years of Study)
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Uropathogenic E. coli (UPEC) is problematic and still the leading cause of urinary tract infections worldwide. It is developed resistance against most antibiotics. The investigation, surveillance system, and efficient strategy will facilitate selecting an appropriate treatment that could control the bacterial distribution. The present study aims to investigate the epidemiology and associated risk factors of uropathogenic E. coli and to study their antibiotic resistance patterns. 1585 midstream urine specimens were collected from symptomatic urinary tract infections (UTI) patients (225 males and 1360 females) admitted to Zakho emergency hospital, Zakho, Kurdistan Region, Iraq from January 2016 until the end of December 2

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Publication Date
Sun Dec 07 2014
Journal Name
Baghdad Science Journal
Genotypic Study of Two Virulence Factors fimH and kpsMTII in Uropathogenic Escherichia coli Isolates from Children Patients with Urinary Tract Infections
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Adhesion (type 1 fimbriae) and host defense avoidance mechanisms (capsule or lipopolysaccharide) have been shown to be prevalent in Escherichia coli isolates associated with urinary tract infections. In this work, 50 uropathogenic Escherichia coli (UPEC) isolated from children with urinary tract infections were genotypically characterized by polymerase chain reaction (PCR) assay. We used two genes; fimH and kpsMTII, both of them previously identified in uropathogenic E.coli (UPEC) isolates. The PCR assay results identified fimH (90.0)% and kpsMTII (72.0)% isolates. In the present study, was also demonstrated that these genes may be included in both or one of them within a single isolate.

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Publication Date
Wed Jan 05 2022
Journal Name
Iraqi Journal Of Science
Virulence Genes Profile of Escherichia coli Isolated from Urinary Catheterized and Non-Catheterized Patients
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The severity of UTI produced by E. coli is due to the expression of a wide
spectrum of virulence factors. In this study the role of E. coli virulence determinants
in the pathogenesis of UTI in urinary catheterized and non-catheterized patients has
been evaluated. The isolates were recovered from 129 patients admitted to the
hospital. Virulence genes of E. coli were detected by polymerase chain reaction
analysis for the prevalence of these virulence factors. The targeted genetic
determinants were those coding for Type 1 fimbriae, Pyelonephritis-Associated Pili
(PAP), Antigen 43 (Ag43), α-Hemolysin and Aerobactin siderophores among the
studied isolates. The prevalence of genes fimH, papC, ang43, hlyA and iutA were<

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Publication Date
Thu Apr 27 2023
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Antibacterial effect of ethyl acetate fraction of Medicago Sativa on Escherichia Coli in urinary tract infections
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Background:  One of the most dominant bacterial infections is urinary tract infection (UTI), both in the community and in the hospital settings Since drug resistance become one of the predominant problems of health worldwide, it is necessary to use new methods to overcome drug-resistant bacteria. In this regard, medicinal plants (Medicago sativa )  are considered one of the richest sources to produce antibiotics.

Aim of the study: evaluate the antibacterial effect of the fraction of ethyl acetate of Medicago Sativa extract against Escherichia Coli isolated from urine samples of patients with urinary tract infections.

Material and methods:

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