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jih-1214
Immunization of White Mice against Hydatid Cyst Using Antigen B and Excretion - Secretion Antigen
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      The aim of this study was  toward the possibility of producing antigen that has  the ability to stimulate the immune response against the infection with the  hydatid cyst. To do so antigens were extracted from sheep hydatid cyst fluid of  Echinococcus granulosus .These were: 1- The hydatid cyst fluid called antigen B. 2- Excretion-secretion called ES antigen. 3-B/ES antigen is a mixture (1:1) of the above two antigens. Three concentrations   (15, 30 and 60 µg/ml) from antigen B/ES were prepared to immunize the white mice (males)  with 20 µg/gr body weight and one booster dose  (10 µg/gr) to stimulate immunity.      The efficiency of these antigen concentrations against secondary infections was investigated by the calculation of the reduction in percentage of cysts numbers        The results revealed that the reduction percentages of cyst in immunized group were 91.5% , 92.3% and  100%  for concentration antigen 15, 30 and 60µg/g for    Thr Immunized groups respectively.. There was a significant difference (p≤0.05) between and the positive control group infection. After 45 days of infection, the sensitivity test showed that the highest cellular immune response occurred after three hours of the injection of the B/ES antigen in the left footpad of the animals. This response was measured through the increase in the thickness of the footpad. The reaction was clear in the positive control and in the infected immunized animals. When the test was repeated after 90 days of the infection, the results were similar to the above, but the reaction was more acute than in the first time especially in the positive control groups of mice.

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Publication Date
Sun Jul 03 2016
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Serum Prostate Specific Antigen level in Women with Polycystic Ovary Syndrome
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Background: Polycystic ovary syndrome (PCOS) is a common disorder in women in the reproductive age associated with disturbance of reproductive, endocrine and metabolic functions. The pathophysiology of PCOS appears to be multifactorial and polygenic.
Objective: To measure the level of prostate –specific antigen (PSA) and to evaluate the value of prostatic specific antigen as a marker of hyperandrogenism in women with PCOS.
Patients and Methods: One hundred women were entrolled in this study, 50 women were PCOS group and the remainder was healthy women served as control group. Hormonal profile (serum testosterone, luteinizing hormone (LH), follicle stimulating hormone (FSH) levels were measured. Ferriman-Gallway Score (FGS) was as

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Publication Date
Thu Jan 30 2014
Journal Name
Al-kindy College Medical Journal
Hydatid Cyst Disease among Patients hospitalized at Baghdad teaching hospital: clinico-epidemiological study
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Background. Echinococcosis/ hydatitdosis is a zoonotic parasitic disease caused by the infestation of the larval form of the tapeworm of the genus Echinococcus .The Liver, lungs, and kidneys are the common areas of infestation.Objectives: To describe hydatid disease in hospitalized patients from a clinico-epidemiological perspectives.Methods:: A retrospective study was conducted over a period of 6 months extending from 15th of November 2011 to the 15th of May 2012 by reviewing records of 125 patients who were hospitalized at Baghdad Teaching Hospital during 2011and received medical and surgical treatment for hydatid cyst disease. The information covered the socio-demographic and clinical characteristics of the patientsResults:.The presen

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Publication Date
Sat Apr 15 2023
Journal Name
Iraqi Journal Of Science
Detection of Adenovirus antigen and its correlation with clinical signs among children with gastroenteritis
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A total of 90 stool sample was collected from patients with gastroenteritis to
detect adenovirus antigen among diarrhea cases. They were tested by general stool
examination (GSE), rapid immunochromatographic test and Enzyme Linked
Immunosorbent Assay (ELISA). GSE showed that adenovirus gastroenteritis
infection resulted in non-bloody diarrhea, the existence of RBCs in 7% and Pus in
37% of the samples, Entamoeba histolytica trophozoite and cyst were seen in 3%
and 2% of the samples respectively. The rapid test showed that 21% of samples
were positive for rotavirus, 8% for adenovirus and 3% for astrovirus. Meanwhile,
the ELISA test showed that adenovirus was positive in 9% of the samples. These
findings establish

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Publication Date
Sun Jan 03 2010
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Surgical Management of a Hydatid Cyst of the Hepatic Dome Ruptured into the Biliary Tree
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Background: Hydatid disease remains an important & challenging medical problem.
Case report: A62 year old male patient was admitted complaining of upper abdominal discomfort. Six days earlier he suffered from acute pain in the right upper quadrant, mild systemic allergic manifestation, a fever of 38.5oC and chills. He was managed by a new surgical approach of myoplasty of the right hemidiaphragm combined with preoperative decompression of the bile duct by an endoscopic sphincterotomy. A case search identified study describing rupture of a hydatid cyst of the hepatic dome into biliary tract. The incidence of intrabiliary rupture of hepatic hydatid cysts is 25% in patients treated with external surgical

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Publication Date
Sun Apr 03 2016
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Detection of Galactomannan Antigen in the Serum of Iraqi Patients with Suspected Invasive Aspergillosis
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Invasive aspergillosis is a severe infection that occurs in patients with prolonged neutropenia, following chemotherapy,transplantation,or immunosuppressive protocols .Galactomannan ( GM) is a molecule ,found in the cell wall of Aspergillus species and is released in the blood during growth .The detection of GM in the blood is used to diagnose the invasive Aspergillosis in humans using ELISA assay.
Objectives: To detect Galactomannan antigen in in the serum of immunocompramized patients suspected to have invasive aspergillosis.
Patients and methods: This study was conducted on 50 patients from the hematology&oncology department,of Baghdad teaching hospital and pediatric oncology wards ,from March 2013 to October 2013.The patien

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Publication Date
Sun Jan 05 2020
Journal Name
Biochemical And Cellular Archives
EFFECT OF HUMAN LEUKOCYTE ANTIGEN HLA DRB1 ON DEVELOPMENT OF BAGHDAD SORE IN PATIENTS
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Leishmania tropica is a species of flagellate parasites that infects humans and the cause of the disease cutaneous leishmaniasis, which is the most common form of leishmaniasis. It is one of the major parasites, which have high prevalence than other parasites in Iraq. The aim was to investigate the role of HLA alleles in susceptibility to cutaneous leishmaniasis infection in Baghdad in a sample of Iraqi patients. Cross sectional study (thirty Iraqi Arab Muslims patients with Leishmania tropica infection and thirty Iraqi Arab Muslims healthy persons) were participated in this study. Patients were consulted Department of Dermatology in Medical city Teaching hospital and AL Yarmook Teaching hospital for the period between March 2014 till May 2

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Publication Date
Thu Dec 19 2024
Journal Name
Journal Of Baghdad College Of Dentistry
BAX In situ hybridization and proliferating cell nuclear antigen immunohistochemical expressions in salivary gland tumours
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Background: Epithelial salivary gland tumours are relatively uncommon and constitute a wide spectrum of variable morphologic and biologic entities. The cell proliferation / death balance is most important in the development of salivary gland tumours. The aim of this study was to examine the expression of PCNA protein immunohistochemically and Bax mRNA gene using in situ hybridization techniques and to correlate between the clinicopathological features of salivary gland tumours with the expressions of PCNA protein and Bax mRNA. Materials and Methods: Forty nine formalin fixed paraffin embedded tissue blocks of epithelial salivary gland tumours were used in this study. Haematoxylin and Eosin stain was used for reassessment of the histopath

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Publication Date
Wed Jan 01 2020
Journal Name
Medico -legal Update
Histological changes in liver tissue resulting from Hydatid cyst infection: Comparison between sheep and cattle in iraq
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Scopus (3)
Scopus
Publication Date
Mon Dec 01 2014
Journal Name
Iraqi Journal Of Science,
Detection of human leukocyte antigen and celiac disease auto antibodies in serum of patients with multiple sclerosis
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To determine the important pathogenic role of celiac disease in triggering several autoimmune disease, thirty patients with Multiple Sclerosis of ages (22-55) years have been investigated and compared with 25 healthy individuals. All the studied groups were carried out to measure anti-tissue transglutaminase antibodies IgA IgG by ELISA test, anti-reticulin antibodies IgA and IgG, and anti-endomysial antibodies IgA and IgG by IFAT. There was a significant elevation in the concentration of anti-tissue transglutaminase antibodies IgA and IgG compared to control groups (P≤0.05), there was 4(13.33%) positive results for anti-reticulin antibodies IgA and IgG , 3(10%) positive results for anti-endomysial antibodies IgA and IgG . There were 4 pos

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Publication Date
Sun Oct 22 2023
Journal Name
Iraqi Journal Of Science
Detection of Human Leukocyte Antigen and Celiac Disease Auto Antibodies in serum of Patients with Multiple Sclerosis
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To determine the important pathogenic role of celiac disease in triggering several
autoimmune disease, thirty patients with Multiple Sclerosis of ages (22-55) years
have been investigated and compared with 25 healthy individuals. All the studied
groups were carried out to measure anti-tissue transglutaminase antibodies IgA IgG
by ELISA test, anti-reticulin antibodies IgA and IgG, and anti-endomysial
antibodies IgA and IgG by IFAT. There was a significant elevation in the
concentration of anti-tissue transglutaminase antibodies IgA and IgG compared to
control groups (P≤0.05), there was 4(13.33%) positive results for anti-reticulin
antibodies IgA and IgG , 3(10%) positive results for anti-endomysial antibodies

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